Isolation and Identification of a PCBs-Degrading Strain

Abstract

<jats:p>A salt-tolerant PCBs degrading strain B2.6 which can use PCBs as the sole carbon source and energy was isolated from the deep sea sediments of southern Yellow Sea. It was identified as Vibrio splendidus, according to morphological, physiological and biochemical characteristics and 16SrDNA sequence analysis. The experimental results suggested that the optimum conditions for the degradation of PCBs are temperature 30°C, pH 7 to 8, the concentration of NaCl was 150g•L-1. Under the optimum conditions, the PCBs degradation could reach 98.27% in 72h which concentration was below 0.6mg•L-1. The genome was amplified with bacterium 16SrDNA primer. The amplified product was purified and sequenced forphylogenetic analyses with Genbank. The bacterium B2.6 was identified as Vibrio splendidus. RAPD analyses of B2.6 produced stable DNA fingerprint. These studies have provided parameter for the further dynamic resolution of bacterium clusters for the rehabilitation of PCBs contaminated underground water environment with B2.6 PRB method.</jats:p>