Isolation and Identification of Actinomycetes from Termite’s Gut against Human Pathogen

Khucharoen, K.; Sripairoj, N.; Sinma, Kanokkorn

doi:10.3923/ajava.2012.68.73

Cited by 30 publications

(17 citation statements)

References 10 publications

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“…Genomic DNA oobtained from the actinomycetes isolates was used as a template for 16S rRNA gene amplification. The amplification was generated using 9F (5'-GAG TTT GAT CCT GGC TCA G-3') and 1541R (5'-AAG GAG GTG ATC CAG CC-3') primers (Khucharoenphaisan et al 2012) [8] by 35 cycle of 1 min at 94 °C, 1 min at 55 °C and 1 min at 72 °C, proceeded by 1 min at 94 °C, 1 min at 55 °C and 4 min at 72 °C. PCR products were purified by PCR purification kit (QIAGEN, Germany) according to the manufacturer's protocol.…”

Section: Molecular Identificationmentioning

confidence: 99%

Identification and screening of rare actinomycetes isolated from Neesia altissima Bl.

Pratiwi¹,

Hidayat²,

Hanafi³

et al. 2017

AIP Conference Proceedings

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A fuzzy logic model to forecast stock market momentum in Indonesia's property and real estate sector AIP Conference Proceedings 1862, 030125 (2017) Abstract. Actinomycetes is the main source of antibiotics and endophytic actinomycetes from medicinal plants has considerable potential as like the host. The aim of this research is to identify rare actinomycetes isolated from Neesia altissima and to screen their antagonistic activity against diarrhea-causing bacteria in order to find new potential secondary metabolites. Samples of N. altissima were collected from mount Halimun-Salak National Park. Endophytic actinomycetes were isolated from roots of N. altissima by surface sterilization method. Screening of antagonistic activity was conducted against five diarrhea-causing bacteria such as Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 25241, Shigella flexneri ATCC 12022, and Staphylococcus aureus ATCC 25923 by using diffusion disc methods. The endophytic actinomycete showed in vitro antibacterial activity against four diarrhea-causing bacteria, except the B. cereus ATCC 10876. The phylogenetic tree generated from 16S rRNA sequence showed that sequence of endophytic actinomycetes isolates nested in the clade belonging to the genus Nonomuraea. Sequence of UICC B-94 formed a monophyletic clade with N. jabiensis strain A4036 and N. rubra strain AC 615. Therefore, it is named as Nonomuraea sp. strain UICC B-94.

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Section: Molecular Identificationmentioning

confidence: 99%

Identification and screening of rare actinomycetes isolated from Neesia altissima Bl.

Pratiwi¹,

Hidayat²,

Hanafi³

et al. 2017

AIP Conference Proceedings

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“…Termites have been used as a biological pointer to assess soil quality and fertility. This is because they play a key part in soil transportation, methanogenesis, nitrogen fixation, acetogenesis, and nutrient circulation, thus improving the soil water content, pH, porosity, and organic carbon content [5][6][7]. Some termite societies like Macrotermes and Coptotermes are found in a mound ( Figure 1) which they built by excavating soil particles from depths using resources from the surrounding soil [8].…”

Section: Introductionmentioning

confidence: 99%

Environmental Sustainability: A Review of Termite Mound Soil Material and Its Bacteria

Enagbonma

Babalola

2019

Sustainability

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The high quantity of nutrients accumulated in termite mound soils have placed termite mound as a 'gold mine' for bacteria concentrations. However, over the years, not much attention has been given to the bacteria present in termite mound soil. This is because many studies have focused on approaches to manage termites which they see as menace to agricultural crops and buildings. Therefore, we aimed to evaluate the potential application of termite mound soil material and its bacteria for biotechnological purposes. This review has been grouped into four key parts: The termite mound as hotspot for bacterial concentration, the degradation of lignocellulose for biofuel production, termite mound soil as a soil amendment, and the role of termite mound soil and its bacteria in bioremediation and bio-filtration. Therefore, the effective usage of the termite mound soil material and its bacteria in an ecofriendly manner could ensure environmental sustainability.

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“…Termites are a group of social insects that are classified as the order of Isoptera, which is closely related to cockroaches [7]. They live in large colonies comprises a king, queen, soldiers and workers, each of which has different behaviors and functions [8,9,10]. Soil termites play an important role in circulating nutrients from decaying dead plant materials in the soil ecosystem [11].…”

Section: Introductionmentioning

confidence: 99%

“…These symbiotic between Actinomycetes and termites provides assisting functions for termites, such as nutrient cycling and exchange, and also protect termites from invading pathogens [13]. Some of these termite-associated Actinomycetes may also exhibit lignin-cellulolytic activity [17] and antagonistic activity against diverse pathogens [10,18]. Furthermore, some novel and prospective novel species of Actinomycetes associated with termites have been reported degraded hemicellulose compound, showed antiviral activity, and produced antibiotic compound such as microtermolides and natalamycin [19,20,21,22].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest

Krishanti¹,

Zulfina

Wikantyoso

et al. 2018

J.Trop.Life.Science

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Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolates were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs. active compounds for further biotechnology application in the medical, pharmaceutical, and agricultural fields.

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Isolation and Identification of Actinomycetes from Termite’s Gut against Human Pathogen

Cited by 30 publications

References 10 publications

Identification and screening of rare actinomycetes isolated from Neesia altissima Bl.

Identification and screening of rare actinomycetes isolated from Neesia altissima Bl.

Environmental Sustainability: A Review of Termite Mound Soil Material and Its Bacteria

Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest

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