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Nypa palm, a kind of palm usually found in mangrove forests in the Pacific Oceans. Bark of Nypa palm contained concentrated dyes that can be used as source of natural dye. The aim of this study was to optimize Natural dye extracted from bark of Nypa Palm by using Central Composite Design (CCD) method. The result showed that the condition of 22-28% Nypa palm bark and heat at 100°C for 20-95 min gave the maximum color on the cotton fabrics after dying. The treatment combinations between bark concentration and extracted time showed that a regression model of optimization of extraction as lightness of dyeing cotton fabrics (L-value) were in agreement to experimental results with R 2 = 0.639. The minimum L-value was between 62-64.