Isolation and identification of anaerobic bacteria from coconut wastewater factory for ethanol, butanol and 2, 3 butanediol production

Lertsriwong, Supattra; Comwien, Jantarush; Chulalaksananukul, Warawut; Glinwong, Chompunuch

doi:10.1016/j.ibiod.2016.11.020

Cited by 9 publications

(2 citation statements)

References 23 publications

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“…The coconut oil in the wastewater pond can be changed into solid fat that then floats to the top of the wastewater and can be eliminated from the wastewater pond and used as a substrate for biogas fermentation [4]. However, the residual coconut milk processing wastewater (CCW) can be a severe environmental and health problem, forming an odor and foul air in the neighborhood, clogging, and harboring pathogens [3,5,6].…”

Section: Introductionmentioning

confidence: 99%

Whole-Cell Biotransformation of 1,12-Dodecanedioic Acid from Coconut Milk Factory Wastewater by Recombinant CYP52A17SS Expressing Saccharomyces cerevisiae

et al. 2020

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Biotransformation of fatty acids from renewable wastewater as feedstock to value-added chemicals is a fascinating commercial opportunity. α,ω-dicarboxylic acids (DCAs) are building blocks in many industries, such as polymers, cosmetic intermediates, and pharmaceuticals, and can be obtained by chemical synthesis under extreme conditions. However, biological synthesis can replace the traditional chemical synthesis using cytochrome P450 enzymes to oxidize fatty acids to DCAs. Saccharomyces cerevisiae BY(2R)/pYeDP60-CYP52A17SS (BCM), a transgenic strain expressing the galactose-inducible CYP52A17SS cytochrome P450 enzyme, was able to grow in a coconut milk factory wastewater (CCW) medium and produced 12-hydroxydodecanoic acid (HDDA) and 1,12-dodecanedioic acid (DDA). The supplementation of CCW with 10 g/L yeast extract and 20 g/L peptone (YPCCW) markedly increased the yeast growth rate and the yields of 12-HDDA and 1,12-DDA, with the highest levels of approximately 60 and 38 µg/L, respectively, obtained at 30 °C and pH 5. The incubation temperature and medium pH strongly influenced the yeast growth and 1,12-DDA yield, with the highest 1,12-DDA formation at 30 °C and pH 5–5.5. Hence, the S. cerevisiae BCM strain can potentially be used for producing value-added products from CCW.

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Section: Introductionmentioning

confidence: 99%

Whole-Cell Biotransformation of 1,12-Dodecanedioic Acid from Coconut Milk Factory Wastewater by Recombinant CYP52A17SS Expressing Saccharomyces cerevisiae

et al. 2020

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“…The predominant bacteria screened by specific pollutants has had good degradation effects on the pollutants. The predominant bacteria for coconut juice processing wastewater has been screened (Lertsriwong et al 2017). It was found that these predominant bacteria can degrade the pollutants to ethanol, butanol, acetone, 2,3-butanediol, and other low mass molecules of organic matter.…”

Section: Introductionmentioning

confidence: 99%

Screening Predominant Bacteria and Construction of Efficient Microflora for Treatment of Papermaking White Water

Lan¹,

Zhang²,

Yang³

et al. 2018

BioResources

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Three strains of bacteria were isolated and purified from activated sludge for white water treatment in the laboratory. These strains were identified as Bacillus subtilis, Bacillus cereus, and Virgibacillus pantothenticus through a morphological analysis, the MIDI Sherlock automatic microbial identification system, and 16S rRNA methods. The results of the construction of efficient microflora for white water showed that a mass percentage ratio of B. subtilis, B. cereus, and V. pantothenticus of 50%:35%:15% achieved an optimal treatment effect. Analysis by gas chromatograph-mass spectrometer (GC-MS) established that the content of characteristic pollutants in white water decreased notably after treatment with the efficient microflora, and detected the intermediate products of short chain fatty acids, alcohols, and other compounds. Moreover, through measuring the removal rate of chemical oxygen demand (COD), electrical conductivity, and cationic demand (CD), the optimal retention time for white water treatment with the efficient microflora was 4 h to 6 h, and when the removal rate of COD reached approximately 90%, the electrical conductivity and the cationic demand were reduced to lower values.

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Augmentation of Bio-butanol Production Through Isolation, Screening and Optimization of Growth and Fermentation Parameters Using Response Surface Methodology

2022

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Isolation and identification of anaerobic bacteria from coconut wastewater factory for ethanol, butanol and 2, 3 butanediol production

Cited by 9 publications

References 23 publications

Whole-Cell Biotransformation of 1,12-Dodecanedioic Acid from Coconut Milk Factory Wastewater by Recombinant CYP52A17SS Expressing Saccharomyces cerevisiae

Whole-Cell Biotransformation of 1,12-Dodecanedioic Acid from Coconut Milk Factory Wastewater by Recombinant CYP52A17SS Expressing Saccharomyces cerevisiae

Screening Predominant Bacteria and Construction of Efficient Microflora for Treatment of Papermaking White Water

Augmentation of Bio-butanol Production Through Isolation, Screening and Optimization of Growth and Fermentation Parameters Using Response Surface Methodology

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