Isolation and Identification of Anticoagulant Components from the Venom of Honey Bee (Apis Mellifera Caucasica)

Abstract

The paper presents experimental data on the separation, identification and isolation of anti-coagulant components of the venom of the honey bee Apis mellifera Caucasica, harvested from apiaries from the ecologically clean zone of Azerbaijan. The protein components of zootoxin with molecular masses 41kD and 20kD, 15kD, corresponding to hyaluronidase and phospholipase was isolated from honey bee venom by the method of gel chromatography on a column with Sephadex G-750 eluting with 0.4M sodium phosphate buffer, followed by spectrophotometric measurement of the unit optical density of the fractions at λ=280nm on a Hitachi-557 spectrophotometer.