Isolation and identification of antioxidant compounds in Vaccinium bracteatum Thunb. by UHPLC-Q-TOF LC/MS and their kidney damage protection

Zhang, Jian; Chu, Chun Jun; Li, Xian Lun; Shi, Yanhong; Yan, Biao; Ren, Hui; Xu, Nai Yu; Liang, Zhitao; Zhao, Zhongzhen

doi:10.1016/j.jff.2014.09.005

Cited by 41 publications

(14 citation statements)

References 19 publications

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“…Our previous studies also reported that chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, orientin, and isoorientin were found in V. bracteatum leaf extracts [15,16]. In addition, Zhang et al [21] reported seven compounds in the leaves of V. bracteatum, namely, isoorientin, orientin, vitexin, isovitexin, isoquercitrin, quercetin-3-O-α-L-rhamnoside, and chrysoeriol 7-O-β-D-glucopyranoside. However, the molecular mechanisms underlying the ability of a single compound isolated from V. bracteatum extract to modulate neurotoxicity is yet to be reported.…”

Section: Discussionmentioning

confidence: 79%

Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons

Kim

Choi

et al. 2021

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Corticosterone (CORT)-induced oxidative stress and neurotoxicity can cause neuronal dysfunction and mental disorders. In the present study, we investigated the effects and mechanism of the HP-20 resin fraction of the water extract of Vaccinium bracteatum leaves (NET-D1602) and its bioactive compound p-coumaric acid on neuronal cell damage in SH-SY5Y cells and primary culture of rat cortical cells. NET-D1602 and p-coumaric acid significantly improved cell viability in CORT-induced neurotoxicity in SH-SY5Y cells and primary cultures of rat cortical cells, and increased the activities of antioxidant enzymes (superoxide dismutase and catalase) against CORT-induced neurotoxicity in SH-SY5Y cells. NET-D1602 and p-coumaric acid increased the phosphorylation levels of ERK1/2 and cAMP response element-binding protein (CREB) in cortical neurons. In addition, CREB phosphorylation by NET-D1602 and p-coumaric acid was dramatically reversed by PKA, c-Raf/ERK, PI3K, and mTOR inhibitors. Lastly, we demonstrated the neuroprotective effects of NET-D1602 (3 and 10 μg/mL) and p-coumaric acid (3 and 10 μM) via increased CREB phosphorylation in CORT-induced neurotoxicity mediated via the ERK1/2, Akt, and mTOR pathways. These results suggest that p-coumaric acid is a potential neuroprotective component of NET-D1602, with the ability to protect against CORT-induced neurotoxicity by regulating ERK1/2, Akt, and mTOR-mediated CREB phosphorylation.

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Section: Discussionmentioning

confidence: 79%

Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons

Kim

Choi

et al. 2021

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“…Ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-Q-TOF MS/MS) is a powerful approach that enables simultaneous determination of multiple components [86,87,88,89,90,91,92]. The chemical structures of FSI constituents could readily be defined by using fragmentation rules, characteristic fragmentation and quasi-molecular ions summarized in the literature, and comparison of retention times and parent and product ions with those of standards.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Microwave-Assisted Extraction Conditions for Five Major Bioactive Compounds from Flos Sophorae Immaturus (Cultivars of Sophora japonica L.) Using Response Surface Methodology

Liu

2016

Molecules

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Microwave-assisted extraction was applied to extract rutin; quercetin; genistein; kaempferol; and isorhamnetin from Flos Sophorae Immaturus. Six independent variables; namely; solvent type; particle size; extraction frequency; liquid-to-solid ratio; microwave power; and extraction time were examined. Response surface methodology using a central composite design was employed to optimize experimental conditions (liquid-to-solid ratio; microwave power; and extraction time) based on the results of single factor tests to extract the five major components in Flos Sophorae Immaturus. Experimental data were fitted to a second-order polynomial equation using multiple regression analysis. Data were also analyzed using appropriate statistical methods. Optimal extraction conditions were as follows: extraction solvent; 100% methanol; particle size; 100 mesh; extraction frequency; 1; liquid-to-solid ratio; 50:1; microwave power; 287 W; and extraction time; 80 s. A rapid and sensitive ultra-high performance liquid chromatography method coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (EIS-Q-TOF MS/MS) was developed and validated for the simultaneous determination of rutin; quercetin; genistein; kaempferol; and isorhamnetin in Flos Sophorae Immaturus. Chromatographic separation was accomplished on a Kinetex C 18 column (100 mmˆ2.1 mm; 2.6 µm) at 40˝C within 5 min. The mobile phase consisted of 0.1% aqueous formic acid and acetonitrile (71:29; v/v). Isocratic elution was carried out at a flow rate of 0.35 mL/min. The constituents of Flos Sophorae Immaturus were simultaneously identified by EIS-Q-TOF MS/MS in multiple reaction monitoring mode. During quantitative analysis; all of the calibration curves showed good linear relationships (R 2 > 0.999) within the tested ranges; and mean recoveries ranged from 96.0216% to 101.0601%. The precision determined through intra-and inter-day studies showed an RSD% of <2.833%. These results demonstrate that the developed method is accurate and effective and could be readily utilized for the comprehensive quality control of Flos Sophorae Immaturus.

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“…To address all the problems related to activated carbon, polymers with nonpolar and polar surfaces, particularly Amberlite XAD-4 based on styrene–divinylbenzene, cyano-functionalized porous polystyrene [ 98 ], biochars functionalized with polyethylimine, orthosilicate, and polyaniline [ 99 ], polyamide resins [ 100 ], aminophenyl boronic acid with uniform-macroporous poly(chloromethylstyrene- co -divinylbenzene), poly(chloromethylstyrene- co -divinylbenzene) [ 101 ], and polystyrene-divinyl sorbents with different degrees of crosslinking such as LiChrolut [ 102 ] or AMBERCHROM [ 103 ] were evaluated for solid-phase extraction of flavonoids from plant extracts.…”

Section: Solid-phase and Membrane Extraction As Purification And Sepamentioning

confidence: 99%

Isolation, Separation, and Preconcentration of Biologically Active Compounds from Plant Matrices by Extraction Techniques

2017

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Isolation and identification of antioxidant compounds in Vaccinium bracteatum Thunb. by UHPLC-Q-TOF LC/MS and their kidney damage protection

Cited by 41 publications

References 19 publications

Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons

Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons

Optimization of Microwave-Assisted Extraction Conditions for Five Major Bioactive Compounds from Flos Sophorae Immaturus (Cultivars of Sophora japonica L.) Using Response Surface Methodology

Isolation, Separation, and Preconcentration of Biologically Active Compounds from Plant Matrices by Extraction Techniques

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