Isolation and Identification of Antioxidant Peptides from Jinhua Ham

Abstract

The antioxidant activities of the peptides extracted from Jinhua ham were evaluated on the basis of hydroxyl radical scavenging activity, DPPH radical scavenging activity, and Fe(2+) chelating ability. The peptide extracts exhibited great hydroxyl radical scavenging activity and DPPH radical scavenging activity as well as Fe(2+) chelating ability at the concentration of 1 mg/mL, which suggested the presence of peptides with antioxidant activity. The peptides were separated using size exclusion chromatography and reversed-phase HPLC. The fraction with highest DPPH radical scavenging activity was further purified and identified using liquid chromatography tandem matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (HPLC-MALDI-TOF/TOF-MS). The sequence of the antioxidant peptide was identified as Gly-Lys-Phe-Asn-Val. The assessment of fractions indicated that the hydrophobic fractions contributed more to free radical scavenging activities than the hydrophilic peptides. It was concluded that natural peptides extracted and isolated from the Jinhua ham by several chromatographic techniques have antioxidant activities.

Stability of Angiotensin I-converting Enzyme Inhibitory Activity of Peptides Extracted from Dry-cured Jinhua Ham

“…Zhu, Zhang [12] reported that the peptide content of Jinhua hams was 1.16% and that these crude peptides also exhibited high antioxidant activity. In the study of Escudero, Mora [26], ACE-inhibitory activity of peptides from Spanish dry-cured ham was also investigated and an inhibition of 60.7% at 10 mg/mL was reported.…”

“…Crude peptides extracted from Jinhua ham were proved to possess DPPH scavenging ability of 77.39% and great antioxidant activity in PC12 cell model [10,11]. Peptide with sequence of GKPNV purified from Jinhua ham has been isolated and assayed to have a great antioxidant activity [12]. However, no studies have investigated the antihypertensive abilities of Jinhua ham peptides (JHP) to date.…”

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

“…However, healthy cells may be damaged by the many side effects of anticancer drugs, and resistance to anticancer drugs has been observed [3]. Therefore, substantial attention is being paid to identifying anticancer drugs with high efficiency and low toxicity from natural sources [4]. …”

The antioxidant activity and transcellular pathway of Asp‐Leu‐Glu‐Glu in a Caco‑2 cell monolayer

“…Dry‐cured ham could experience extensive proteolysis, producing functional peptides during the long processing period (Zhang et al ., ; Zhu et al ., ). Xing et al .…”