Isolation and Identification of Antioxidant Substances from the Stems of Butterbur (Petasites japonicus)

김민영,; 이정현,; 황윤이,; 송경식,; 전미라,

doi:10.3746/jkfn.2008.37.8.979

Cited by 8 publications

(4 citation statements)

References 10 publications

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“…1) was isolated from the ethanolic extract of Petasites japonicus stems in the previous study. 22 Quercetin (QC) was obtained from Sigma-Aldrich Co. (St. Louis, MO, USA) and used as a positive control. Optical density was measured using a microplate reader (BioTek Instruments, Winooski, VT, USA).…”

Section: Generalmentioning

confidence: 99%

“…Kaempferol (3,4 0 ,5,7-tetrahydroxyavone, KF), a avonol, has been isolated from a number of natural sources, including strawberries, 18 Ginkgo biloba leaves (EGb 761), 19 Pu-erh tea, 20 paprika, 21 and butterbur (Petasites japonicus). 22 This compound exerts a number of biological actions, including anti-inammatory, anticarcinogenic, anti-diabetic, and neuroprotective activities. For example, 100 mM KF attenuates the interleukin-1b (IL-1b)-induced proliferation of rheumatoid arthritis synovial broblasts as well as the production of cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), and matrix metalloproteinases (MMPs).…”

Section: Introductionmentioning

confidence: 99%

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Kaempferol attenuates the glutamate-induced oxidative stress in mouse-derived hippocampal neuronal HT22 cells

et al. 2014

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It is thought that the neuronal cell loss caused by oxidative stress is the primary mechanism underlying the pathogenesis of several neurodegenerative disorders. Glutamate is an endogenous neurotransmitter, but at high concentrations it can act as a neurotoxicant by increasing the intracellular levels of reactive oxygen species (ROS). Therefore, the development of factors that can attenuate glutamate-induced oxidative stress in neuronal cells is a good strategy by which new drugs could be discovered that may treat or prevent neurodegenerative diseases. Here, the neuroprotective effects of kaempferol (KF) isolated from the stems of butterbur (Petasites japonicus) were examined in glutamate-treated hippocampal neuronal cells (HT22). The administration of KF (25 μM) resulted in a significant increase in cell viability (105.18 ± 7.48%) compared with the control (100.00 ± 3.05%), while glutamate (5 mM) reduced cell viability by 39.94 ± 1.61%. The glutamate-induced calcium (Ca(2+)) influx (1.93 ± 0.08-fold) was significantly reduced by 0.89 ± 0.02-fold following the administration of 25 μM KF. Additionally, when HT22 cells were stressed with excessive glutamate, there was a 3.70 ± 0.01-fold increase in intracellular ROS generation, even though this was effectively attenuated by KF (25 μM, 0.72 ± 0.01-fold). The protective effects of KF in HT22 cells were later confirmed using a lactate dehydrogenase (LDH) assay and a FITC-annexin V/propidium iodide double staining procedure. These findings also revealed that the neuroprotective effects of KF are a result of the regulation of the expression levels of proteins, such as Bcl-2, Bid, apoptosis-inducing factor (AIF), and mitogen-activated protein kinase (MAPK). This is the first report to investigate the neuroprotective influence of KF in glutamate-treated HT22 cells. These data demonstrate that KF may be a useful candidate for pharmacological therapies that can prevent and treat neurodegenerative diseases such as Alzheimer's disease (AD).

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Section: Generalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kaempferol attenuates the glutamate-induced oxidative stress in mouse-derived hippocampal neuronal HT22 cells

et al. 2014

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“…( 24 ) In P. japonicus , petaslignolide A is purified a new furofuran lignan with antioxidant activity. ( 4 ) Kim et al ( 26 ) purified and isolated kaempferol as the active compounds of the stems of P. japonicus . The antioxidant activity of the active compound was examined by DPPH radical scavenging assay, thiobarbituric acid-reactive substance (TBARS) assay in the linoleic acid model system, and lipoxygenase inhibition assay.…”

Section: Antioxidant Compounds and In Vitro Antioxmentioning

confidence: 99%

“…The antioxidant activity of the active compound was examined by DPPH radical scavenging assay, thiobarbituric acid-reactive substance (TBARS) assay in the linoleic acid model system, and lipoxygenase inhibition assay. ( 26 ) Moreover, several compounds such as caffeoylquinic acids and its isomer, quercetin, kaempferol glycosides, and fukinolic acid in the leaves and roots were identified. Among them, 3,5-di- O -caffeoylquinic acid exhibited the greatest radical-scavenging capacity, as assessed using an HPLC system with post-column online antioxidant detection based on 2-2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS + ) radical-scavenging activity.…”

Section: Antioxidant Compounds and In Vitro Antioxmentioning

confidence: 99%

Antioxidant compounds of <i>Petasites japonicus</i> and their preventive effects in chronic diseases: a review

Hiemori-Kondo

2020

J. Clin. Biochem. Nutr.

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Petasites japonicus ( P. japonicus ) is a plant of the Asteraceae family. Its roots and stems have been used for the treatment or the prophylaxis of migraine and tension headache as a traditional Chinese medicine in Japan and Korea. Sesquiterpenoids, lignans, and flavonoids are components of P. japonicus . Regarding the biological activity of P. japonicus , its anti-allergic effect has been researched extensively using IgE antigen-stimulated degranulation of RBL-2H3 cells or passive cutaneous anaphylaxis reaction in experimental animal models. The study of the antioxidant activity of P. japonicus was initiated approximately 15 years ago using in vitro assays. In addition, its in vivo effect has also been examined in animal models with induced oxidative injury. Moreover, recently, many types of antioxidant compounds have been rapidly and simultaneously identified using the liquid chromatography–mass spectrometry technique. The number of reports on the other functions of this plant, such as its neuroprotective and anti-inflammatory effects, has been increasing. In this review, I summarized the studies of functional foods derived from P. japonicus , which may provide a basis for the development of potential functional foods. Finally, I discuss the future research avenues in this field.

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A review on the ethnobotany, phytochemistry, pharmacology and toxicology of butterbur species (Petasites L.)

Kulinowski

Luca

Minceva

et al. 2022

Journal of Ethnopharmacology

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Isolation and Identification of Antioxidant Substances from the Stems of Butterbur (Petasites japonicus)

Cited by 8 publications

References 10 publications

Kaempferol attenuates the glutamate-induced oxidative stress in mouse-derived hippocampal neuronal HT22 cells

Kaempferol attenuates the glutamate-induced oxidative stress in mouse-derived hippocampal neuronal HT22 cells

Antioxidant compounds of <i>Petasites japonicus</i> and their preventive effects in chronic diseases: a review

A review on the ethnobotany, phytochemistry, pharmacology and toxicology of butterbur species (Petasites L.)

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