Isolation and Identification of Antioxidants from Methanol Extract of Sword Bean (Canavalia gladiata)

Kim, Jong-Pil; Lee, Hyang-Hee; Moon, Jae‐Hak; Ha, Dongryong; Kim, Eun Sun; Kim, Jin Hwan; Seo, Kyewon

doi:10.9721/kjfst.2013.45.6.777

Cited by 9 publications

(4 citation statements)

References 23 publications

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“…Malondialdehyde content was determined using the thiobarbituric acid method [27]. The flavonoid content was determined using the colorimetric method [28].…”

Section: Calculations Of Soluble Protein Proline Malondialdehyde and ...mentioning

confidence: 99%

Changes in growth, physiology, and photosynthetic capacity of spinach (Spinacia oleracea L.) under different nitrate levels

et al. 2023

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Nitrate content is an essential indicator of the quality of vegetables but can cause stress at high levels. This study aimed to elucidate the regulatory mechanisms of nitrate stress tolerance in spinach (Spinacia oleracea L.). We studied the effects of exogenous application of 15 (control), 50, 100, 150, 200, and 250 mM NO3- on spinach growth, physiology, and photosynthesis. The results showed that all the nitrate treatments inhibited the growth of the aerial parts of spinach compared to the control. In contrast, low nitrate levels (50 and 100 mM) promoted spinach root formation, but this effect was inhibited at high levels (150, 200, and 250 mM). Treatment with 150 mM NO3- significantly decreased the root growth vigor. Low nitrate levels increased the chlorophyll content in spinach leaves, whereas high levels had the opposite effect. High nitrate levels also weakened the net photosynthetic rate (Pn), the actual photochemical efficiency of PSII Y(II), and increased non-photochemical quenching (NPQ), reducing photosynthetic performance. Nitrate stress increased the activity of nitrate reductase (NR) and promoted the accumulation of nitrate in spinach leaves, exceeding the health-tolerance limit for nitrate in vegetables, highlighting the necessity of mitigating nitrate stress to ensure food safety. Starting with the 150 mM NO3- treatment, the proline and malondialdehyde content in spinach leaves and roots increased significantly as the nitrate levels increased. Treatment with 150 mM NO3- significantly increased soluble protein and flavonoid contents, while the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were significantly reduced in leaves. However, spinach could resist nitrate stress by regulating the synthesis of osmoregulatory substances such as proline, thus showing some nitrate tolerance. These results provide insights into the physiological regulatory mechanisms of nitrate stress tolerance and its mitigation in spinach, an essential vegetable crop.

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“…Malondialdehyde content was determined using the thiobarbituric acid method [27]. The flavonoid content was determined using the colorimetric method [28].…”

Section: Calculations Of Soluble Protein Proline Malondialdehyde and ...mentioning

confidence: 99%

Changes in growth, physiology, and photosynthetic capacity of spinach (Spinacia oleracea L.) under different nitrate levels

et al. 2023

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“…Therefore, it is judged to show anti-allergic efficacy by reducing the expression of allergy-related cytokines. C. gladiate also contains medicinal ingredients such as urease, hemaglutinine, canavanine, canavalia gibberellin I and II [47], and these are considered to inhibit allergic reactions in combination.…”

Section: Conflicts Of Interestmentioning

confidence: 99%

Anti-Allergic Effect of Fermented Extracts of Medicinal Plants Andrographis paniculate, Salvia plebeia R. Br., Canavalia gladiate, Eleuthorococcus senticosus, Ulmus davidiana var. japonica, and Clerodendrum trichotomum Thunb. ex Murray

Choi¹,

Kim²

2022

Microbiol. Biotechnol. Lett.

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Since the main symptoms of COVID-19 involve the respiratory system, the infection rate of this disease is predicted to be higher in patients with other respiratory conditions such as allergic rhinitis. In such a situation, it will be meaningful to conduct research on an allergy treatment that has fewer side effects and can effectively reduce allergy symptoms. Here, we prepared experimental samples under various fermentation conditions with mixed extracts of six medicinal plants. To examine the anti-allergic efficacy of these samples, an egg albumin-induced allergic rhinitis animal model experiment, a serum histamine and IgE experiment, and a COX and LO inhibitory activity experiment were conducted. As a result of animal experiments, OVA+SP-4 showed superior efficacy compared to OVA+SP-1 in nasal rubbing and sneezing experiments and had anti-allergic efficacy similar to that of OVA-cetirizine. The serum histamine concentration of OVA+SP-4 was also 1.3 times higher than that of the OVA+cetirizine group, showing a high histamine reduction ability, and IgE showed the same trend. An analysis of COX inhibitory efficacy also confirmed that COX-1 and COX-2 inhibitory efficacy is high, and the longer the fermentation time, the higher the antiallergic efficacy. The composition proposed by this study is expected to have a significant effect on sustainable allergy prevention and treatment in the future by applying it to human patients.

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“…Phenolic compounds are the most common of the various bioactive components in plant foods, and they have high antioxidant activities. In previous studies, the phenolic compounds of sword bean were gallic acid, methyl gallate, monogalloyl hexoside, digalloyl hexoside, digallic acid, trigalloyl hexoside, gallotannin, and protocatechuic acid [10,35,36]. These gallic acid derivatives possess various biological functions such as antioxidant activity, antimicrobial effect, anticancer effect, and hepatoprotective effect [37][38][39][40].…”

Section: Bioactive Compoundsmentioning

confidence: 99%

Roasting and Cryogenic Grinding Enhance the Antioxidant Property of Sword Beans (Canavalia gladiata)

Jung

Rhee

2020

J. Microbiol. Biotechnol.

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Coffee is one of the most widely consumed non-alcoholic beverages in the world. Caffeine is the most important ingredient of the drink, and a 150 ml cup of coffee contains about 60-120 mg of caffeine. However, caffeine has various effects on the central nervous system and is thought to be the most widely used psychoactive substance. It also increases respiration rate, causes bronchodilation, stimulates lipolysis, and increases diuretic action [1]. As a result of the health concerns mentioned above, many people are replacing caffeinated coffee with decaffeinated coffee as a part of their effort to avoid caffeine; however, decaffeinated coffee also contains a small amount of caffeine [2]. This means that substitution with decaffeinated coffee to eliminate caffeine consumption may not be effective in patients on a caffeine-restricted or abstinence diet. Because of these problems, research continues to find coffee substitutes using a variety of crops. Lee et al. [3] studied small, roasted black beans as a coffee substitute for reducing metabolic bone disease in climacteric women. Moreover, in Guatemala in the 1980s, roasted and ground sword bean seeds were consumed as a coffee-like drink [4]. Likewise, roasted sword bean, called King bean, is consumed as a substitute for coffee in Korea. Since the only such beans commercially available are processed by roasting and grinding, consumers do not have many options in terms of flavor and function. Sword bean (Canavalia gladiata), a perennial vine plant of the bean family, is the largest edible bean plant of the family. It originates in the tropical regions of Southeast Asia and is suitable for cultivation in the southern region of Korea [5]. It has been used as a food and medicinal plant in Asia for thousands of years, and mature seeds are often roasted, ground, and consumed as a drink [6]. Sword bean is rich in phytochemicals like saponin, tannin, flavonoids, terpenoids, and steroids as well as in nutrients such as carbohydrates, proteins, vitamins, and minerals [7, 8]. It also contains urease, hemaglutinine, canavanine, and canavalia gibberellin I and II. The seeds, pods, stems and roots are used in folk remedies that are effective against dysentery, nausea, hemorrhoids, sinusitis, backaches, and obesity. Furthermore, recent studies have shown that sword bean possesses physiological functions such as antioxidant, anti-inflammatory, hematopoietic expansion-improving, hepatoprotective, and anti-angiogenic activities [9-13]. While development and distribution of functional beverages using legumes have been attempted, they have not received much response from consumers due to the peculiar beany flavor [3]. Therefore, to use them as a substitute for coffee beverages, it is necessary to develop a process that can improve the beany flavor of legumes. The objective of this study was to optimize the conditions for enhancing the antioxidant properties of sword bean (Canavalia gladiata) as a coffee substitute in two processing methods, roasting and grinding. The optimum conditions ...

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Isolation and Identification of Antioxidants from Methanol Extract of Sword Bean (Canavalia gladiata)

Cited by 9 publications

References 23 publications

Changes in growth, physiology, and photosynthetic capacity of spinach (Spinacia oleracea L.) under different nitrate levels

Changes in growth, physiology, and photosynthetic capacity of spinach (Spinacia oleracea L.) under different nitrate levels

Anti-Allergic Effect of Fermented Extracts of Medicinal Plants Andrographis paniculate, Salvia plebeia R. Br., Canavalia gladiate, Eleuthorococcus senticosus, Ulmus davidiana var. japonica, and Clerodendrum trichotomum Thunb. ex Murray

Roasting and Cryogenic Grinding Enhance the Antioxidant Property of Sword Beans (Canavalia gladiata)

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