Isolation and identification of antiplasmodial N-alkylamides from Spilanthes acmella flowers using centrifugal partition chromatography and ESI-IT-TOF-MS

In vitro antiplasmodial activity of indole alkaloids from the stem bark of Geissospermum vellosii

“…In ongoing investigation of plants used for the treatment of malaria (Grace et al, 2010;Graziose et al, 2011;Mbeunkui et al, 2011), we report the isolation and identification of three indole alkaloids and two bis-indole alkaloids with antiplasmodial activity from the stem bark of Geissospermum vellosii. The isolated compounds were identified by LC/MS and NMR analysis.…”

Isolation and structural elucidation of indole alkaloids from Geissospermum vellosii by mass spectrometry

“…The solvent system for alkaloid separation by HPCCC was optimized as described previously [23]. The settling time of the selected solvent system HEMWat SS4 [ethyl acetate-butanol-water (2:3:5, v/v/v)] did not exceed 20 s, which is consistent with a successful HPCCC separation [25].…”

“…Freeze-dried stem bark was first cut into small pieces using a knife and then ground into a fine powder using a Waring stainless steel blender (Waring Commercial, Torrington, CT). The powdered material (200 g) was extracted at room temperature three times each with 3 L methanol for 3 days as described previously [23]. The filtered extract was concentrated using a rotary evaporator (BUCHI Corporation, New Castle, DE).…”

“…The methanol crude extract (2 g) was separated by counter current chromatography with 2:3:5 extended HEMWat (ethyl acetate:butanol:water) solvent system selected as described previously [23]. Elution-extrusion method was performed with the upper phase as stationary phase.…”

“…Elution-extrusion method was performed with the upper phase as stationary phase. After solvent system equilibration in the columns as described earlier [23], the methanol crude extract in 30 mL of 1:1 (v/v) mixture of each phase of the solvent system was injected through the sample loop. The mobile phase was pumped into the inlet of the preparative columns (900 mL) at a flow rate of 10 mL/min for 90 min and the stationary phase at the same flow rate for another 90 min.…”