Isolation and identification of arboviruses from the Sultanate of Oman

Al-Busaidy, Suleiman; Mellor, P. S.

doi:10.1017/s095026880004855x

Cited by 22 publications

(17 citation statements)

References 22 publications

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“…Culicoides midges were suspected to be responsible for this fast spread of SBV because they are known vectors for several related viruses (e.g. AKAV) (St George et al ., ; Al‐Busaidy & Mellor, ; Bryant et al ., ; Yanase et al ., ). Several studies using field‐collected midges have detected SBV in C. obsoletus complex, C. chiopterus and C. dewulfi midges (De Regge et al ., ; Rasmussen et al ., ; Goffredo et al ., ; Elbers et al ., , ; Larska et al ., , ) and a vector competence study has shown the replication and dissemination of SBV in C. sonorensis (Veronesi et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Culicoides monitoring in Belgium in 2011: analysis of spatiotemporal abundance, species diversity and Schmallenberg virus detection

Regge

Deken

Fassotte

et al. 2015

Medical Vet Entomology

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Abstract. In 2011, Culicoides (Diptera: Ceratopogonidae) were collected at 16 locations covering four regions of Belgium with Onderstepoort Veterinary Institute (OVI) traps and at two locations with Rothamsted suction traps (RSTs). Quantification of the collections and morphological identification showed important variations in abundance and species diversity between individual collection sites, even for sites located in the same region. However, consistently higher numbers of Culicoides midges were collected at some sites compared with others. When species abundance and diversity were analysed at regional level, between-site variation disappeared. Overall, species belonging to the subgenus Avaritia together with Culicoides pulicaris (subgenus Culicoides) were the most abundant, accounting for 80% and 96% of all midges collected with RSTs and OVI traps, respectively. Culicoides were present during most of the year, with Culicoides obsoletus complex midges found from 9 February until 27 December. Real-time reverse-transcription polymerase chain reaction screening for Schmallenberg virus in the heads of collected midges resulted in the first detection of the virus in August 2011 and identified C. obsoletus complex, Culicoides chiopterus and Culicoides dewulfi midges as putative vector species. At Libramont in the south of Belgium, no positive pools were identified.

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Section: Introductionmentioning

confidence: 99%

Culicoides monitoring in Belgium in 2011: analysis of spatiotemporal abundance, species diversity and Schmallenberg virus detection

Regge

Deken

Fassotte

et al. 2015

Medical Vet Entomology

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“…The presence of SBV has in the meantime also been confirmed in the Netherlands, Belgium, United Kingdom, France, Luxembourg, Italy and Spain. The rapid and wide expansion of SBV, together with the knowledge that related viruses like Akabane and Aino virus are spread by midges and mosquitoes (St George et al., ; Al‐Busaidy and Mellor, ; Bryant et al., ; Yanase et al., ), led to the hypothesis that also SBV might be spread by these vectors. We analysed pools of midges caught in Belgium to identify putative local vectors for SBV.…”

Section: Introductionmentioning

confidence: 99%

Detection of Schmallenberg virus in differentCulicoidesspp. by real-time RT-PCR

Regge

Deblauwe

Deken

et al. 2012

Transboundary and Emerging Diseases

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Summary:To identify possible vectors of Schmallenberg virus (SBV), we tested pools containing heads of biting midges (Culicoides) that were caught during the summer and early autumn of 2011 at several places in Belgium by real time RT-PCR. Pools of heads originating from following species: C. obsoletus complex, C. dewulfi and C. chiopterus were found positive, strongly indicating that these species are relevant vectors for SBV.

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“…Vector competence studies for these arboviruses are most complete for AKAV, particularly in Australia where the role of Culicoides brevitarsis in transmitting and spreading the virus has been characterized in detail [7], [8]. Incidence of AKAV in field collected Culicoides has also been studied in Israel [9], [10], the Oman [11] and the Republic of South Africa [12], where the major Afro-tropical arbovirus vector Culicoides imicola is believed to play a primary role in transmission.…”

Section: Introductionmentioning

confidence: 99%

Implicating Culicoides Biting Midges as Vectors of Schmallenberg Virus Using Semi-Quantitative RT-PCR

et al. 2013

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BackgroundThe recent unprecedented emergence of arboviruses transmitted by Culicoides biting midges in northern Europe has necessitated the development of techniques to differentiate competent vector species. At present these techniques are entirely reliant upon interpretation of semi-quantitative RT-PCR (sqPCR) data in the form of Cq values used to infer the presence of viral RNA in samples.Methodology/Principal FindingsThis study investigates the advantages and limitations of sqPCR in this role by comparing infection and dissemination rates of Schmallenberg virus (SBV) in two colony lines of Culicoides. Through the use of these behaviorally malleable lines we provide tools for demarcating arbovirus infection and dissemination rates in Culicoides which to date have prevented clear implication of primary vector species in northern Europe. The study demonstrates biological transmission of SBV in an arthropod vector, supporting the conclusions from field-caught Culicoides and provides a general framework for future assessment of vector competence of Culicoides for arboviruses using sqPCR.Conclusions/SignificanceWhen adopting novel diagnostic technologies, correctly implicating vectors of arboviral pathogens requires a coherent laboratory framework to fully understand the implications of results produced in the field. This study illustrates these difficulties and provides a full examination of sqPCR in this role for the Culicoides-arbovirus system.

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Isolation and identification of arboviruses from the Sultanate of Oman

Cited by 22 publications

References 22 publications

Culicoides monitoring in Belgium in 2011: analysis of spatiotemporal abundance, species diversity and Schmallenberg virus detection

Culicoides monitoring in Belgium in 2011: analysis of spatiotemporal abundance, species diversity and Schmallenberg virus detection

Detection of Schmallenberg virus in differentCulicoidesspp. by real-time RT-PCR

Implicating Culicoides Biting Midges as Vectors of Schmallenberg Virus Using Semi-Quantitative RT-PCR

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