Isolation and identification of Bacillus subtilis strain YB-05 and its antifungal substances showing antagonism against Gaeumannomyces graminis var. tritici

Purification and Characterization of a Thermostableβ-Mannanase fromBacillus subtilisBE-91: Potential Application in Inflammatory Diseases

“…The homology between BE-91 16S rDNA (gi 260159552) and B. subtilis 16S rDNA (gi 530330588 and gi 341831474) was 99%. It was confirmed that the similarity of B. subtilis type strains about 16S rRNA gene sequence is higher than 98% [27, 28]. We also obtained ≥98% similarity to 16S rRNA gene sequences of B. subtilis isolates.…”

Isolation and characterization of plant growth promoting bacteria (PGPB) from anaerobic digestate and their effect on common wheat (Triticum aestivum) seedling growth

“…In this study, Bacillus isolates (MAD-05, Bacillus subtilis and TAD-05, Bacillus licheniformis) showed high antifungal activity of 62.75% and 43.53% PGI, which may contribute to the competition for space and nutrients and secretion of antifungal compounds (Yang et al, 2015). Similarly, Bacillus species have been widely reported to have antifungal activity against a wide variety of phytopathogens (Ji et al, 2014;Kumar et al, 2012;Liu et al, 2016).…”

Identification of lipopeptides produced by Bacillus subtilis Czk1 isolated from the aerial roots of rubber trees

“…Yang et al (2015) found that B. subtilis YB05 contains at least four antifungal genes (fenB, ituA, hag, and tas); the other antifungal genes tested (spaS, sfp, and mycB) may also be present but were not detectable using the primers and PCR conditions used in this study. The antimicrobial peptide (AMP) biosynthetic genes srfAA (surfactin), bacA (bacilysin), fenD (fengycin), bmyB (bacillomycin), spaS (subtilisin), and ituC (iturin) have been examined in 184 Bacillus spp isolates obtained from plant environments, including aerial, rhizosphere, and soil environments in Spain (Mora et al, 2011).…”

“…When B. subtilis YB-05 was grown in liquid culture medium under specific conditions, six main types of antifungal substances were detected through analysis by mass spectrometry (Yang et al, 2015). The difference between the number of antifungal genes and the number of the expressed products in the culture supernatant can be attributed to the specific liquid culture growth conditions that may not have permitted the production of the final products of the hyphae (Yang et al, 2015). Therefore, PCR can provide the basis for follow-up to MALDI-TOF-MS analysis.…”

“…Bacillus spp are often regarded as optimum candidates for the control of plant diseases because they can secrete antifungal compounds (Pathak and Keharia, 2014;Yang et al, 2015). The majority of Bacillus strains can produce antimicrobial compounds.…”