Isolation and identification of chromium reducing bacteria from tannery effluent

Sanjay, Maria Susan; Dorairaj, Sudarsanam; Raj, Gnanaprakasam Antony; Baskar, Kathirvelu

doi:10.1016/j.jksus.2018.05.001

Cited by 74 publications

(43 citation statements)

References 22 publications

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“…Anthropogenic activities are the major factors that contribute to increased amount of chromium (Cr) concentrations in the soil and water (Sanjay et al , Tseng et al ). Among various forms of heavy metal contamination, Cr contamination is of significant scientific interest as it affects plant productivity as well as human health (Rehman et al , Ge et al , Singh and Prasad ).…”

Section: Introductionmentioning

confidence: 99%

“…Anthropogenic activities are the major factors that contribute to increased amount of chromium (Cr) concentrations in the soil and water (Sanjay et al 2018, Tseng et al 2019). Among various forms of heavy metal contamination, Cr contamination is of significant Abbreviations -BSO, L-buthionine sulfoximine; CAT, catalase; CDNB, 1-chloro-2, 4-dinitrobenzene; DES, L-cysteine desulfhydrase; GSH, glutathione; GST, glutathione-S-transferase; HA, hydroxylamine; HT, hypotaurine; NBT, nitroblue tetrazolium; qP, photosynthetic quantum yield; ROS, reactive oxygen species; SOD, superoxide dismutase.…”

Section: Introductionmentioning

confidence: 99%

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Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Kushwaha

Singh

2019

Physiologia Plantarum

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In plants, investigation on heavy metal toxicity and its mitigation by nutrient elements have gained much attention. However, mechanism(s) associated with nutrients‐mediated mitigation of metal toxicity remain elusive. In this study, we have investigated the role and interrelation of glutathione (GSH) and hydrogen sulfide (H2S) in the regulation of hexavalent chromium [Cr(VI)] toxicity in tomato (Solanum lycopersicum), pea (Pisum sativum) and brinjal (Solanum melongena) seedlings, supplemented with additional sulfur (S). The results show that Cr(VI) significantly reduced growth, total chlorophyll and photosynthetic quantum yield of tomato, pea and brinjal seedlings which was accompanied by enhanced intracellular accumulation of Cr(VI) in roots. Moreover, Cr(VI) enhanced the generation of reactive oxygen species in the studied vegetables, while antioxidant defense system exhibited differential responses. However, additional supply of S alleviated Cr(VI) toxicity. Interestingly, addition of l‐buthionine sulfoximine (BSO, a glutathione biosynthesis inhibitor) further increased Cr(VI) toxicity even in the presence of additional S but GSH addition reverses the effect of BSO. Under similar condition, endogenous H2S, l‐cysteine desulfhydrase (DES) activity and cysteine content did not significantly differ when compared to controls. Hydroxylamine (HA, an inhibitor of DES) also increased Cr(VI) toxicity even in the presence of additional S but sodium hydrosulfide (NaHS, an H2S donor) reverses the effect of HA. Moreover, Cr(VI) toxicity amelioration by NaHS was reversed by the addition of hypotaurine (HT, an H2S scavenger). Taken together, the results show that GSH which might be derived from supplied S is involved in the mitigation of Cr(VI) toxicity in which H2S signaling preceded GSH biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Kushwaha

Singh

2019

Physiologia Plantarum

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“…Similar results were reported by Patra et al (2010) that the ChR gene in Arthrobacter aurescens, Bacillus atrophaeus, and Rhodococcus erythropolis was 268 bp. Research by Sanjay et al (2018) showed that Klebsiella pneumoniae and Mangrovibacter yixingensis also had a ChR gene of 268 bp. The ChR gene encodes an enzyme that catalyzes the reduction reaction of Cr (VI) to Cr (III) (Wani and Adeosun 2017).…”

Section: The Potency Of Endophytic Bacteria In Phosphate Solubilizingmentioning

confidence: 99%

The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil

et al. 2020

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Abstract. Rohmah NS, Suharjono, Jatmiko YD, Siswanto D, Mustafa I. 2020. The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil. Biodiversitas 21: 1920-1927. Endophytic bacteria tolerant of chromium have the potential to reduce Cr (VI) to Cr (III) and produce compounds that can improve plant survival in Cr (VI) contaminated soils. This study was performed to isolate and identify Cr (VI)-tolerant bacteria from the endosphere of Ficus septica Burm.F growing on the tannery waste contaminated soil, Screening of bacteria was carried out based on the level of Cr (VI) tolerance. High tolerant isolates were tested for their potential of Indole Acetic Acid (IAA) and exopolysaccharide (EPS) production, phosphate solubilizing, and the presence of chromate reductase (ChR) genes. The most potential bacterial isolate was identified based on 16S rDNA sequences. The results showed that three isolates (E3, E4, and E8) were tolerant of Cr (VI) up to 900 mg/L. E8 isolates produced the highest IAA production (20.05 μg/mL), phosphate solubilization (127.78 μg/mL), and EPS production (9,70 μg/mL), and all isolates had the ChR gene. E8 isolate was found to be 100% identical to Enterobacter cloacae ATCC 13047.

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“…The reduction of hexavalent Cr to trivalent Cr is a viable process, mediated by ChrR enzymes under aerobic conditions via its cytosolic form and in anaerobic respiration through its membrane-bound component [12] . Sanjay et al [13] have isolated two bacterial species from tannery effluent, identified as Klebsiella pneumoniae and Mangrovibacter yixingensis which showed the presence of a chromate reducing gene by PCR, which could find potential application in Cr bioremediation. The toxicity of Cr 6+ and accumulation of reduced Cr 3+ culminating in the formation of toxic chromium-DNA adducts in the nucleus are shown in Figure1.…”

Section: Introductionmentioning

confidence: 99%

Microbial Chromate Reductases: Novel and Potent Mediators in Chromium Bioremediation-A Review

Mala¹,

Takeuchi²,

Mani³

2020

Applied Microbiology: Theory ＆ Technology

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Heavy metal pollution from the growing industrialization are a significant cause of environmental concern.Chromium (Cr) is commonly used in the production of stainless steel, textile dyeing, electroplating, as nuclear coolants and largely in chrome tanning of hides and skins. About 90% of leather is produced by chrome tanning and the leather industry contributes to an overload of Cr toxicity in tannery effluents. Accumulation of Cr6+ is carcinogenic, genotoxic and teratogenic to organisms. Biological methods are ‘green’ approaches for chromium bioremediation and microorganisms are the desired candidates for pollution abatement. Microbial chromate reduction is mediated by chromate reductases (ChrRs) which may be expressed constitutively or inducibly. ChrRs have been produced by a number of bacteria, fungi and yeasts and may be extracellular or localized in the membrane or cytosol. ChrRs are dependent on electron donors such as reduced Nicotinamide adenine dinucleotide (NADH) or reduced Nicotinamide adenine dinucleotide phosphate (NADPH) or reduced Glutathione (GSH) as cofactors. In chromate reduction by ChrRs, Cr6+ undergo one electron transfer to producean unstable Cr5+ radical that is converted to stable and less toxic Cr3+. Putative ChrR genomic sequences have been studied with 99% sequence similarity in Gram negative bacteria. ChrRs are valuable resources in different environments for chromate reduction. This review is to discuss the expression and characteristics of ChrRs and their mechanisms in reduction of Cr6+ toxicity in order to provide a comprehensive understanding of this novel class of enzymes for promisingapplications in Cr bioremediation.

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Isolation and identification of chromium reducing bacteria from tannery effluent

Cited by 74 publications

References 22 publications

Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

Glutathione and hydrogen sulfide are required for sulfur‐mediated mitigation of Cr(VI) toxicity in tomato, pea and brinjal seedlings

The potency of endophytic bacteria isolated from Ficus septica as phytoremediation promoting agent of Cr (VI) contaminated soil

Microbial Chromate Reductases: Novel and Potent Mediators in Chromium Bioremediation-A Review

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