Isolation and Identification of Colletorichum musae from Imported Bananas

Lim, Jin Young; Lim, Tae Heon; Jin, Byeong

doi:10.5423/ppj.2002.18.3.161

Cited by 28 publications

(27 citation statements)

References 3 publications

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“…Moreover, appressorium characteristics of the isolates were almost like those of C. musae characteristics, including colony shape on PDA, and conidial color confirmed that the isolates are of C. musae. Our findings in the current study are in agreement with the results of Lim et al (2002). The pathogenic isolates showed cultural characteristics on bananas, i.e., colony color and growth texture, that were morphologically similar to that previously described for the banana pathogen C. musae (Sutton, 1980;Price, 1995;Photita et al, 2005).…”

Section: Discussionsupporting

confidence: 92%

“…Long transportation and storage period at markets may make bananas vulnerable to disease attack (Thompson and Burden, 1995). Several symptoms have been reported on banana fruits, many of which may occur during transportation and storage, because of unhealthy ripening and/or because symptomless fruits never receive any treatment before transportation and marketing (Lim et al, 2002). The genus Colletotrichum and its teleomorph Glomerella are considered to be major plant pathogens worldwide.…”

Section: Introductionmentioning

confidence: 99%

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First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia

Abd-Elsalam¹,

Roshdy²,

Amin³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Colletotrichum musae is the causal agent of anthracnose in banana fruits; infection by this fungal pathogen results in severe post-harvest losses. Eleven C. musae isolates were obtained from infected imported banana fruit samples with anthracnose lesions collected from different markets in Riyadh, Saudi Arabia. The pathogenic, morphological, cultural, and molecular characteristics of these C. musae isolates were evaluated. The cultures had characteristic fast-growing sparse aerial mycelia that were white, with copious cinnamon conidial masses, conidia usually elliptical, and setae absent. An inoculation test was used to determine whether isolates could cause anthracnose symptoms on banana fruits. Necrotic lesions developed and orange-colored spore structures were later observed on these lesions. Microsatellite-primed PCR (MP-PCR) was used to identify genetic variation among the C. musae isolates. The dendrogram obtained from cluster analysis of the MP-PCR fingerprints revealed a great deal of homogeneity among the isolates, shown by the formation of two clusters. Intraspecific similarity among the C. musae isolates ranged from 83 to 100%. This is the first report demonstrating morphological and genetic variation within a population of C. musae in Saudi Arabia.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia

Abd-Elsalam¹,

Roshdy²,

Amin³

et al. 2010

Genet. Mol. Res.

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“…Although C. musae was not prevalent in the present study, several studies have shown that C. musae is the most common species associated with anthracnose of banana. In Korea and Saudi Arabia, C. musae was associated with anthracnose of imported banana (Lim et al, 2002;Abd-Elsalam et al, 2010). Thangamani et al (2011) reported that according to morphological and physiological characterization, C. musae was pathogenic to banana in India.…”

Section: Discussionmentioning

confidence: 99%

“…C. musae was not a common species associated with several types of local banana cultivars, although C. musae has been reported to be the most common causal anthracnose of many banana cultivars worldwide (Lim et al, 2002;Abd-Elsalam et al, 2010;Thangamani et al, 2011). C. gloeosporioides is the most prevalent species associated with anthracnose of banana, suggesting the potential of cross-infection to other tropical fruits.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia

Sakinah¹,

Suzianti²,

Zakaria³

2014

Genet. Mol. Res.

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ABSTRACT. Anthracnose caused by Colletotrichum species is a common postharvest disease of banana fruit. We investigated and identified Colletotrichum species associated with anthracnose in several local banana cultivars based on morphological characteristics and sequencing of ITS regions and of the β-tubulin gene. Thirty-eight Colletotrichum isolates were encountered in anthracnose lesions of five local banana cultivars, 'berangan', 'mas', 'awak', 'rastali', and 'nangka'. Based on morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioides and 6 isolates as C. musae. C. gloeosporioides isolates were divided into two morphotypes, with differences in colony color, shape of the conidia and growth rate. Based on ITS regions and β-tubulin sequences, 35 of the isolates were identified as C. gloeosporioides and only 3 isolates as C. musae; the percentage of similarity from BLAST ranged from 95-100% for ITS regions and 97-100% for β-tubulin. C. gloeosporioides isolates were more prevalent compared to C. musae. This is the first record of C. gloeosporioides associated with banana anthracnose in Malaysia. In a phylogenetic analysis of the combined dataset of ITS regions and β-tubulin using a maximum likelihood method, C. gloeosporioides and C. musae isolates were clearly separated into two groups. We concluded that C. gloeosporioides and C. musae isolates are associated with anthracnose in the local banana cultivars and that C. gloeosporioides is more prevalent than C. musae.

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“…The optimum pH for mycelial growth was investigated by using PDA media. Their pH was adjusted from 5.0 to 10.0 by increments of one (Lim et al 2002). Cultural characteristics of the fungus were evaluated from the growth performance on culture plates.…”

Section: Cultural Characteristicsmentioning

confidence: 99%

Fulfillment of Koch’s postulates for in vitro pathogenicity of Musicillium Theobromae (Turconi) Zare & w. Gams as the cause of banana cigar end rot disease

Masudi¹,

Bonjar²

2012

Journal of Plant Protection Research

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Pathogenicity of Musicillium theobromae (Turconi) Zare & W. Gams (syn. Stachylidium theobromae Turconi, Verticillium theobromae E.W. Mason & S. Hughes) causing Banana cigar end rot disease has fulfilled Koch's Postulates for the first time. To assess and prove the pathogenicity, M. theobromae mycelial discs and conidia were inoculated in triplicate to surface-disinfested green banana fruits. The bananas were infected both through artificial wounds as well as through intact unwounded fruits. After inoculation, the bananas were covered with moist cotton and incubated for 30 days at 23°C. Plain moist cotton was used on the controls. Those inoculated treatments kept under high relative humidity exhibited symptoms of banana cigar end rot disease. The controls and those kept under low relative humidity did not develop symptoms and remained healthy. The results indicated that both optimum temperature and high relative humidity are necessary for infection onset and disease development. It was also determined that infection can occur both through wounds and intact unwounded fruit epidermis. The cultural characteristics in M. theobromae were also investigated. It was found that the best mycelial growth occurred on potato dextrose agar (PDA) and the optimum pH for mycelial growth was 8.

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Isolation and Identification of Colletorichum musae from Imported Bananas

Cited by 28 publications

References 3 publications

First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia

First morphogenetic identification of the fungal pathogen Colletotrichum musae (Phyllachoraceae) from imported bananas in Saudi Arabia

Phenotypic and molecular characterization of Colletotrichum species associated with anthracnose of banana (Musa spp) in Malaysia

Fulfillment of Koch’s postulates for in vitro pathogenicity of Musicillium Theobromae (Turconi) Zare & w. Gams as the cause of banana cigar end rot disease

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