Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk

Gangwal, Anjali; Kashyap, SK; Katiyar, Sushil; Meena, D. S.; Boyal, Pramod; Gupta, Kapil

doi:10.22207/jpam.11.1.41

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…Staphylococcus produces alpha haemolysin causes a narrow zone of complete haemolysis and beta haemolysin produces a wider zone of partial or incomplete heamolysis or both (double haemolysis) or no haemolysis in some other bacteria (Cowan, 1993) [2] . The results of the present study are in complete agreement with that of Khichar and Kataria (2007) [6] ; Upadhyay and Kataria, (2010) [14] , Gangwal et al (2016) [3] who did record hemolytic activity of S. aureus obtained from milk samples of cattle and goats. The sugar fermentation studies were carried out by using API biochemical test kit for S. aureus.…”

Section: Resultssupporting

confidence: 92%

Detection of Staphylococcus aureus from bovine mastitis milk

Choudhary,

Kashyap

2024

Int. J. Adv. Biochem. Res.

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Mastitis, an inflammation of the mammary gland. Staphylococcus aureus is the most common cause of economic loss in dairy cattle due to decrease quality and quantity of milk. This study was aimed to find-out the prevalence of S.aureus in case of bovine mastitis (clinical) through conventional method. Absolute diagnosis of pathogens plays great role in cure of disease. A total of 27 S. aureus isolates were isolated from 50 individual milk samples from dairy cows with clinical mastitis. The results showed that they were positive in gram staining (forming a bacteria cluster of purple cocci), catalase test, coagulase test, blood agar haemolysis, and DNase test. The mannitol, trehalose, sucrose, maltose, and lactose tests were also positive. The prevalence of bacterial isolates in conventional detection were 55%.

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Section: Resultssupporting

confidence: 92%

Detection of Staphylococcus aureus from bovine mastitis milk

Choudhary,

Kashyap

2024

Int. J. Adv. Biochem. Res.

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“…As the present study is focused on development of a rapid and early detection method, a multiplex PCR was devised to allow the simultaneous detection of multiple pathogens of mastitis in a single reaction, which requires smaller amounts of reagents and even lesser time than simplex PCR [5,16]. This multiplex PCR method appeared as a useful tool to resolve bacterial aetiology of mastitis milk samples simultaneously and at the earliest [12,17,18].…”

Section: Discussionmentioning

confidence: 99%

Identification of Bovine Mastitis Associated Pathogens by Multiplex PCR

Sk¹

2017

JDVS

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“…Moreover, the “no-growth”samples are problematic for mastitis laboratories, veterinarians, and dairy producers ( Shome et al., 2011 ). This may be due to less number of bacteria in milk, not growing in standard media or substances in milk that inhibit their growth ( Gangwal et al., 2017 ). Reliable identification methods that are fast and accurate still remain a necessity.…”

Section: Discussionmentioning

confidence: 99%

High throughput Luminex beads based multiplex assay for identification of six major bacterial pathogens of mastitis in dairy animals

Shrinet

Chhabra

Sharma

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionBovine mastitis is caused by over 150 different microorganisms. Specific identification and quantification of multiple bacteria in a single milk sample becomes essential for rapid intervention.MethodsIn the present study a Luminex beads based multiplex assay emphasizing on the precise identification of six major bacterial pathogens of mastitis was developed. Assay was developed in two triplex sets, triplex 1 comprised of Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis while triplex 2 consisted of Staphylococcus aureus, E. coli and Klebsiella pneumoniae.ResultsThe analytical sensitivity was 10 6 copies per reaction mixture for all the six bacteria. A 100% analytical specificity was observed for simultaneous detection of these bacteria. Clinical milk samples from 100 bovine quarters were tested for validation.DiscussionThe analytical sensitivity was similar to the findings reported earlier in real time PCR multiplex assay targeting the DNA of the 11 most common bacterial species or groups in mastitis. The analytical specificity of the optimized assay was 100% similar to reported earlier for simultaneous detection of Mycoplasma spp. and for seven entric viruses of humans.The developed assay indicates a concept proof of a rapid, cost effective high throughput diagnostic tool for identification of major bacteria causing mastitis.

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Isolation and Identification of Common Mastitis Causing Pathogens from Clinical Bovine Mastitic Milk

Cited by 4 publications

References 9 publications

Detection of Staphylococcus aureus from bovine mastitis milk

Detection of Staphylococcus aureus from bovine mastitis milk

Identification of Bovine Mastitis Associated Pathogens by Multiplex PCR

High throughput Luminex beads based multiplex assay for identification of six major bacterial pathogens of mastitis in dairy animals

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