Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria

Abstract

Abstract:This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.

Biodesulfurization of Fossil Fuels: Analysis and Prospective

“…, Rhodococcus Spp., Sphingomonas subarctica , Bacillus Spp., Desulfovibrio desulfuricans , Pyrococcus Spp., Desulfomicrobium scambium , Desulfovibrio longreachii , or Pantoea agglomerans for the desulfurization of whole crude oil under aerobic or anaerobic conditions ( Adegunlola et al ., 2012 ; Gunam et al ., 2013 ; Yang et al ., 2016 ). The advantage of BDS for the desulfurization of whole crude oil is that it can reduce the cost of the desulfurization treatment in refineries ( El-Gendy and Nassar, 2018 ; Al-Jailawi et al , 2015 ).…”