Disinfectants are widely used to get rid of microorganisms whether in hospitals, health centers or for normal domestic use. Some suggested that when disinfectants are incorrectly diluted the disinfectant might promote the growth of antibiotic-resistant bacteria, therefore, in this study pathogenic bacterium (Pseudomonas aeruginosa), isolated from patient with urinary tract infection, treated with two locally popular disinfectants (Claradone and Sarttol). Results showed that the bacterial growth was affected by both disinfectants. The lowest concentration of Claradone that inhibit the growth of this bacterium is considered as the minimum inhibitory concentration (MIC), this was 30%, while the lowest effecting concentration of Sarttol was 3%. A number of survival colonies after treated with high concentration of Calarodone and Sarttol were investigated for their susceptibility to antibiotics, using standard disc diffusion method. Results indicated that these colonies of P. aeruginosa resisted antibiotics they were sensitive to before treatment. So it can be concluded that using Claradone and sarttol can make the pathogenic bacterium (P. aeruginosa) resist some antibiotics.

Ten thermophillic bacterial isolates were screened for their ability to produce biosurfactant depending on the emulsification activity, emulsification index and surface tension. The results showed that Geobacillus thermoleovorans (JQ 912239) was the most efficient one for biosurfactant production. The chemical composition of partial purified biosurfactant revealed that it consists of 37.7% lipids, 26.2% carbohydrate and 10.7% protein. The partial and/or purified biosurfactant was subjected to Fourier Transform Infrared Spectroscopy (FTIR), High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC) and Nuclear Magnetic Resonance (NMR) to complete the chemical characterization. The results of the FTIR confirmed that the biosurfactant contains the lipid, carbohydrate and protein. The HPLC analysis revealed that the lipid fraction consists of palmitic acid, stearic acid and oleic acid, the carbohydrate part consists of xylose, mannose and maltose and the protein part consists of three amino acids (aspartic acid, glutamic acid and glutamine). The lipid fraction was further analyzed using GC and NMR. GC analysis indicated that it contains palmitic acid methyl ester (c16:0), stearic acid (c18:0) and oleic acid (c18:1n9c) however, purified biosurfactant contains a high percentage of palmitic acid methyl ester. The NMR analysis for purified biosurfactant detected that the main component of lipid fraction is triglycerides.

Abstract:This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.

No abstract

No abstract