Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) Having Potential Secondary Metabolites as Anti-Breast Cancer Activity

Yunianto, Prasetyawan; Rosmalawati, Syofi; Rachmawati, Indra; Suwarso, Wahyudi Priyono; Sumaryono, Wahono

doi:10.5454/mi.6.1.4

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…The size of the molecular weight of the two isolates was 700 bp. This result was different from the DNA band size of two endophytic fungi isolates isolated from dahlia plants, which were only 583 and 537 bp in size (Rakhmana et al, 2017), as well as the DNA size of endophytic fungi isolates from srikaya plants was 600 bp (Yunianto et al 2012). Fig.…”

Section: Molecular Identificationmentioning

confidence: 65%

Molecular Identification of Endophytic Fungi from Local Rice and Growth Test on Several Types of Culture Media

et al. 2019

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Local rice is rice that has been cultivated for generations by the community and commonly cultivated without using chemical inputs. Endophytic fungi are fungi that live in the plant tissue and does not cause disease symptoms in the host plants. This study aimed to molecular identifying isolates of MDTA and MDTB endophytic fungi which have been isolated from the local Pulu Mandoti rice plant tissue and growth test on the four types of culture media those were synthetic PDA, natural PDA, MPA, and MEA. The fungi DNA isolation using DNesay Kit. DNA sequencing analysis using the mega BLAST program showed that the MDTB fungus has similarities to Podoscypha bolleana strain 32034 no accession JQ675334 and Podoscypha bolleana strain 32032 no accession JQ675332, whereas the MDTA fungus has similarities to Coprinopsis cinerea A2S3-5 isolate and Coprinopsis cinerea strain CNRMA / F 07-32. The best culture media and sporulation of endophytic fungi is MPA media. This research is the first study to molecular identifying with endophytic fungi from local rice and viability test on the four types of culture media. The results of this study contribute to the diversity of local rice endophytic fungi in Sulawesi.

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Section: Molecular Identificationmentioning

confidence: 65%

Molecular Identification of Endophytic Fungi from Local Rice and Growth Test on Several Types of Culture Media

et al. 2019

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“…The in vitro cytotoxicity testing of compounds 1 and 2 against murine leukemia P-388 cells and human breast cancer cell lines (MCF-7 and T47D) were conducted using MTT assay according to the method previously described. [10][11][12] These experiments were analysed with linear regression.…”

Section: In Vitro Cytotoxicity Testingmentioning

confidence: 99%

Cytotoxic Activity of Two Compounds Extracted from Artocarpus elasticus Reinw. Ex Blume Leaves on Selected Cancer Cell Lines

2021

TJNPR

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“…Isolasi jamur endofitik dilakukan dengan metode Yunianto et al (2012). Jamur endofitik diisolasi dari tanaman jagung sehat bagian akar, batang, dan daun.…”

Section: Metode Penelitianunclassified

Identifikasi dan Kemampuan Jamur Endofitik Asal Jagung (Zea mays L.) Terhadap Patogen Busuk Pelepah (Rhyzoctonia solani)

Rotasouw

Taribuka

Amanupunyo

2020

JBDP

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Maize is a cereal crop which is an important source of food because it is the second source of carbohydrates after rice. Maize cultivation is faced with various plant diseases, that can reduce the quantity and quality of crop yield. Plant disease is one of the limiting factors for crop production and seed quality. One of the most harmful maize diseases is blight or midrib rot caused by the soil-borne fungus Rhizoctonia solani. The aim of the study was to obtain endophytic microorganisms in maize and to test the antagonistic ability of these fungi as biological control agents against R. solani. The study took place in January-September 2018 in the field and the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, University of Pattimura. Endophytic fungi isolated from maize plants from the roots, stems and leaves. Samples were cleaned in running water and air-dried. Subsequently, the samples were sterilized using 70% alcohol and immersed in sodium hypochlorite solution for 2 minutes and rinsed with sterile water 3 times. The sterilized roots, stems and leaves of maize were peeled, cut to a size of ±5 mm, split and dried on sterile filter paper. Sterile samples were grown on PDA culture media at four points and incubated at room temperature. Visual identification was carried out with the aid of a microscope on fungi growing on the culture media. The results showed the presence of the endophytic fungi Trichoderma sp., Aspergillus sp., Penicillium sp., Diplodia sp. and Mucor sp. These fungal species showed different inhibitory powers, which included mechanisms for direct parasitizing of pathogens, producing antibiotics and competition for space and nutrients. Keywords: antagonism, corn, endophytic fungi, identification, pathogenic fungus, Rhizoctonia solani ABSTRAK Jagung (Zea mays L.) merupakan tanaman serelia yang termasuk bahan pangan penting karena merupakan sumber karbohidrat kedua setelah beras. Salah satu kendala dalam budidaya tanama jagung adalah penurunan produksi akibat serangan patogen penyebab penyakit. Untuk menekan perkembangan penyakit dilakukan pengendalian dengan menggunakan mikroorganisme endofitik asal akar, batang dan daun jagung sebagai agensia pengendalian hayati. Penelitian ini bertujuan mengisolasi dan mengidentifikasi mikroorganisme endofitik asal jagung secara morfologi, mendapatkan mikroorganisme endofitik yang memiliki kemampuan antagonis terhadap jamur Rhizoctonia solani penyebab penyakit busuk pelepah pada jagung. Penelitian berlangsung dalam 2 tahap, yaitu di lapangan dan di Laboratorium Hama dan Penyakit Tumbuhan Fakultas Pertanian, Universitas Pattimura. Sampel tanaman diperoleh dari beberapa kebun petani di wilayah Kota Ambon. Hasil penelitian yang diperoleh adalah bahwa jamur endofitik Trichoderma sp, Aspergillus sp, Penicillium sp, Diplodia sp dan Mucor sp. memiliki daya penghambatan yang berbeda-beda, yaitu 78.57% (Trichoderma sp), 65.86% (Penicillium sp), 43.29% (Aspergillus sp), 36.71 (Diplodia sp) dan 34.44% (Mucor sp). Kata kunci: antagonisme, identifikasi, jagung, jamur endofitik, jamurpatogenik, Rhizoctonia solani

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Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) Having Potential Secondary Metabolites as Anti-Breast Cancer Activity

Cited by 10 publications

References 12 publications

Molecular Identification of Endophytic Fungi from Local Rice and Growth Test on Several Types of Culture Media

Molecular Identification of Endophytic Fungi from Local Rice and Growth Test on Several Types of Culture Media

Cytotoxic Activity of Two Compounds Extracted from Artocarpus elasticus Reinw. Ex Blume Leaves on Selected Cancer Cell Lines

Identifikasi dan Kemampuan Jamur Endofitik Asal Jagung (Zea mays L.) Terhadap Patogen Busuk Pelepah (Rhyzoctonia solani)

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