Isolation and Identification of Epizootic Diarrhea Virus in Pigs Under Outbreak at a Large Farm

Strizhakova, O.M.

doi:10.15389/agrobiology.2013.4.65eng

Cited by 3 publications

(7 citation statements)

References 2 publications

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“…Глобальное распространение PEDV продолжается. Усилия МЭБ и национальных служб ветеринарной медицины по локализации и контролю над ЭДС малоэффективны (Song et al, 2005;Martelli et al, 2008;Puranaveja et al, 2009;Strizhakova, 2013;Gerber et al, 2014;Wang et al, 2014;Vlasova et al, 2014;Dastjerdi et al, 2015).…”

Section: Introductionunclassified

“…Заболеваемость и летальность у новорожденных поросят до 5-дневного возраста могут достигать 100%, однако с увеличением возраста до 14-15 дней, летальность снижается до 3-5%. Гибель свиней в результате PEDV-инфекции возможна в любом возрасте, однако у животных на откорме летальный исход имеет споради-ческий характер и не представляет экономической угрозы для хозяйства (Martelli et al, 2008;Strizhakova, 2013;Carvajal et al, 2014;Dastjerdi et al, 2015;Bjustrom-Kraft et al, 2016).…”

Section: Introductionunclassified

“…ЭДС признана МЭБ и ФАО одним из пяти основных вирозов наряду с АЧС, КЧС, РРСС и цирковирозом, представляющих наибольшую экономическую опасность для свиноводста. PEDVоболочечный одноцепочный РНК-вирус (оцРНК), подверженный частым мутациям (как и все оцРНК вирусы), что приводит к рецидивированию диарейного синдрома и стабилизации эпизоотического очага инфекции, с эндемической формой протекания инфекционного процесса, а неординарно высокая контагиозность возбудителя и его исключительная патогенность для новорожденных поросят приводит к глобализации инфекционной патологии (Martelli et al, 2008;Strizhakova, 2013;Gerber et al, 2014;Goede et al, 2015;Diel et al, 2016).…”

Section: Introductionunclassified

“…Реальное прогрессивное распространение данной инфекции показывает крайнюю необходимость применения паллиативных методов борьбы с PEDV, которые могут включать в себя компоненты стратегии «обратного скармливания» и ветеринарно-санитарные меры по эрадикации вируса во внешней среде. Существующие вакцинные препараты в условиях широкого эспериментального применения являются малоэффективными, а иногда оказывают негативное влияние, не обеспечивая надежный протективный иммунитет как у новорожденных поросят, так и у промышленного стада Stevenson et al, 2013;Strizhakova, 2013;Goede and Morrison, 2016;Langel et al, 2016;Tun et al, 2016;Srijangwad et al, 2017).…”

Section: Introductionunclassified

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Changes in the elemental composition of winter wheat plants caused by the action of Megafol and retardants

Miroshnichenko¹,

Makoveychuk²,

Mykhalska³

et al. 2017

Regul. Mech. Biosyst.

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In the conditions of the field experiments conducted during the 2015–2016 vegetation seasons retardants Medax Top, 1.0 l/ha (prohexadione-Са and mepiquat-chloride), appeared more effective than Terpal, 1.5 l/ha (mepiquat-chloride and ethephon), on field plots with high-yield varieties. Foliar application of Megafol promoted the productivity of winter wheat varieties Smuglyanka and Podolyanka and reduced the negative influence of retardants on the wheat plants in the vegetation seasons which were characterized by moisture deficit. The influence of modern retardants – prohexadione-Ca + mepiquat-chloride (Medax Top) and mepiquat-chloride + etefone (Terpal, both BASF, Germany) on the accumulation of some macro- and micronutrients in winter wheat plants was determined. The assays were performed on an ICP-MS Agilent 7700x mass spectrometer (Agilent Technologies, USA) with ICP-MS Mass Hunter WorkStation. Samples of winter wheat plants were taken in the phase of flowering and grain ripening. The samples were dried, homogenized, 0.400 gof weight was dissolved in ICP-grade nitric acid in the Milestone Start D (Milestone Inc., USA). All solutions were prepared on 1st class water (18 MΩ cm–1) obtained on the Scholar-UV Nex Up 1000 (Human Corporation, Korea) water purification system. The ICP-MS Complete Standard IV-ICPMS-71A was used as the external standard, and the internal standard was Sc, both of Inorganic Ventures, USA. According to the ICP-MS results of plant samples of winter wheat of Smuglylanka and Podolanka, it has been shown that, in conditions of wheat growing on light soils of Polissya, modern compositional retardants affect the ionome of plants during vegetation, as well as change the content of inorganic elements in the grain. It was found that winter wheat of middle-stem Podolyanka type, of the intensive type, reacted more responsively to retardant treatment than the short-stem-type highly intensive Smuglyanka type. At the same time, there was an increase in the content of nutrients in winter wheat of the Podolyanka variety. It should be noted that wheat grain of the Smuglyanka variety contained a significantly higher pool of macro- and trace elements than the Podolyanka variety. It was established that the treatment of winter wheat Podolyanka varieties by mepiquat-chloride and ethephon (Terpal, 1.5 l/ha) positively influenced the accumulation of potassium, magnesium and calcium and manganese, copper and zinc in the grain. We found that foliar application of compositions of amino acids simultaneously with retardants can reduce the negative action of PGR’s on formation of the harvest in conditions of drought and influence the maintenance of composition of inorganic elements in the plants and in the grain. The composition of retardants with the content of cyclohexadione derivative (Medax Top) also significantly influenced the changes in the content of inorganic elements in plants and grains. We note the significant increase in the content of magnesium in the leaves and in grain due to the action of Medax Top. These differences in reaction of winter wheat varieties to the action of retardants of the class of cyclohexadione are important for clarifying the systems of nutrition of crops in intensive growing technologies, especially in the conditions of drought and high temperatures. Reduction in the pools of a number of inorganic elements in plants, for example iron, by the action of retardants may be due to the constraints on the entry of these elements from the poor soils of the Polissya zone and should be compensated via foliar nutrition. Also, the peculiarities of the activity of plant growth regulators have been shown to indicate a close relationship between signal systems of plants and changes in ionome.

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Section: Introductionunclassified

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Changes in the elemental composition of winter wheat plants caused by the action of Megafol and retardants

Miroshnichenko¹,

Makoveychuk²,

Mykhalska³

et al. 2017

Regul. Mech. Biosyst.

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“…Changes in the S glycoprotein gene play an important role, since this protein is important for tissue tropism and virulence [5]. A preliminary animal study with PEDV/Belgorod/dom/2008 demonstrated that this recombinant is highly virulent in unvaccinated suckling piglets [21,22].…”

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Molecular characteristics of a novel recombinant of porcine epidemic diarrhea virus

2019

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Porcine epidemic diarrhea (PED) is a contagious viral disease in pigs, caused by the coronavirus porcine epidemic diarrhea virus (PEDV). PEDV infection results in significant mortality in piglets in unvaccinated herds. Like many others RNA viruses, PEDV has high evolutionary rate and is prone to genetic mutations. In this study, we analyzed the complete genome sequence of the recently sequenced isolate PEDV/Belgorod/dom/2008. A recombination event in S gene of PEDV/Belgorod/ dom/2008 was detected. Pairwise identity analysis of the whole genome sequences revealed that PEDV/Belgorod/dom/2008 is an intermediate between PEDV and transmissible gastroenteritis virus (TGEV) strains. These results can be used for further analysis of the evolutionary variability, prevalence, and epidemiology of the porcine epidemic diarrhea virus.

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The characteristics, emergent properties and manner of spread in Ukraine of the Porcine Epidemic Diarrhea Virus

Масюк

Nedzvetsky

Sosnitskiy

et al. 2018

Regul. Mech. Biosyst.

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Study of the emergent properties and paths of spreading of PEDV was carried out in a model experiment on newborn non-immune piglets obtained from a PED virus-free pig-breeding enterprise. The piglets were kept in separate specialized containers, with a volume of 1.0 m3, with access only through the opening at the top of the containers. The experimental group of the animals was infected with PEDV isolate extracted on one of the pig farms from the central region of Ukraine. Infection was carried out orally in a dose of 1–10 genome equivalents of virions. The control piglets, which were situated in the same room as the infected animals, were not deliberately infected. The study of biological material from piglets was carried out using the methods of bacteriology, histology and RT-PCR. To confirm the capability of PEDV to spread through house flies, specimens of Musca domestica vicina Mcq. were caught in the building where the experiment was carried out. The washings from the surface of their bodies were collected with sterile saline. Individually, 28 specimens of flies were selected. They were divided into two parts and the amount of virus in the homogenate of the fly bodies in these groups was determined with an interval between measurements of 72 hours. Study of PEDV in the washings and in flies body homogenates were carried out using RT-PCR. It is established that the field strain PEDV, belonging to the North American grouping II of the second group of the PED virus strains, is an emergent highly pathogenic agent for non-immune newborn piglets. In the model of piglets’ infection it is established that the tested PEDV strain has a high virulence for newborn piglets, DCL is 1–10 virions and the incubation period is 18–26 hours. PED is acute with lethality to 100% within 68–72 hours after infection. According to the results of RT-PCR in washing from the surface of the bodies of flies, it is established that one of the ways piglets are infected and the environment contaminated with the PED virus is the spread of the pathogen by the flies M. domestica vicina Mcq. This leads to the induction of the emergent form of PED in piglets. The presence of PEDV in the homogenate from bodies of M. domestica caught in the focus of infection and the absence of virus reproduction in their body confirms the role of the house fly in the mechanical spread of PEDV in the external environment.

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Isolation and Identification of Epizootic Diarrhea Virus in Pigs Under Outbreak at a Large Farm

Cited by 3 publications

References 2 publications

Changes in the elemental composition of winter wheat plants caused by the action of Megafol and retardants

Changes in the elemental composition of winter wheat plants caused by the action of Megafol and retardants

Molecular characteristics of a novel recombinant of porcine epidemic diarrhea virus

The characteristics, emergent properties and manner of spread in Ukraine of the Porcine Epidemic Diarrhea Virus

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