Isolation and identification of fibrinolytic protease-producing microorganisms from Red Oncom and Gembus, Indonesian fermented soybean cakes

Abstract

Aims: This study was to isolate, screen, and identify microorganisms from fermented food Red Oncom and Gembus that can produce fibrinolytic proteases. Methodology and results: Forthy-three isolates showed proteolytic activities on skim milk agar, while thirthy-eight of them showed fibrinolytic activities both on a fibrin plate and a fibrinogen zymography. The isolates that showed activity in fibrin plate and fibrinogen zymography with lower molecular weight and considered as safe were chosen and identified as Bacillus licheniformis and Bacillus pumilus by using API CHB kit and 16S rRNA. The novel fibrinolytic microorganisms were referred to as B. licheniformis RO3 and B. pumilus 2.g. Conclusion, significance and impact of study: Red Oncom and Gembus are potential sources of microbial fibrinolytic protease. This is the first time that Red Oncom and Gembus as Indonesian fermented foods based on soybean cake were shown having fibrinolytic microorganism. Microbial fibrinolytic enzymes from these fermented foods can be used for functional food formulation to prevent thrombosis and other related diseases.

The Effect of Tempeh gembus on Malondialdehyde and Superoxide Dismutase Enzyme Levels in Rats with Diet-Induced Metabolic Syndrome

“…These kinds of food have been studied to explore source of functional food like red oncom that can be used as potential media for fibrinogenolytic protease production. 2 Like red oncom, tempeh gembus is an Indonesian fermented food derived as a by-product of tofu manufacture from soybeans. As a by-product of tofu manufactures, tempeh gembus is inexpensive, but it contains several nutrients including the isoflavones genistein (57.1 µg/g) and daidzein (33.1 µg/g) which are antioxidants.…”

“…27,14,31 Another benefit of the consumption of tempeh gembus may be related to the occurrence of a fibrinolytic enzyme and this thrombolytic agent could prevent the development of cardiovascular disease. 2,3 Further study of tempeh gembus showed that there are five new protein fractions that may have benefit to health and its hydrolyzate can be used as antimicrobial and functional food to prevent the cardiovascular disease. 5,33,6…”