Isolation and identification of high-yielding alkaline phosphatase strain: a novel mutagenesis technique and optimization of fermentation conditions

Bo, Le; Kang, Xin; Chen, Zuohui; Zhao, Yue; Wu, Si; Li, Jie; Bao, Shijun

doi:10.1080/10826068.2023.2188412

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Furthermore, ARTP has been utilized to generate mutant strains of alkaline phosphatase-producing bacteria, such as Bacillus amyloliquefaciens S-52 [ 33 ]. The alkaline phosphatase activity of the mutant strain S-52 was determined to be 12,110.6 U/L, which is 4.67 times greater than that of the original strain.…”

Section: Application Of Artp To Bacteriamentioning

confidence: 99%

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Zhang

Miao

Feng

et al. 2023

CIMB

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Atmospheric and room-temperature plasma (ARTP) is an efficient microbial mutagenesis method with broad application prospects. Compared to traditional methods, ARTP technology can more effectively induce DNA damage and generate stable mutant strains. It is characterized by its simplicity, cost-effectiveness, and avoidance of hazardous chemicals, presenting a vast potential for application. The ARTP technology is widely used in bacterial, fungal, and microalgal mutagenesis for increasing productivity and improving characteristics. In conclusion, ARTP technology holds significant promise in the field of microbial breeding. Through ARTP technology, we can create mutant strains with specific genetic traits and improved performance, thereby increasing yield, improving quality, and meeting market demands. The field of microbial breeding will witness further innovation and progress with continuous refinement and optimization of ARTP technology.

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Section: Application Of Artp To Bacteriamentioning

confidence: 99%

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Zhang

Miao

Feng

et al. 2023

CIMB

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Isolation, Identification, and Fermentation Optimization of Phytase-Producing Bacteria and Their Effects on Soybean Seedlings

Zhang,

Song,

Guo

et al. 2025

Appl Biochem Biotechnol

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Engineering Bacterial Chitinases for Industrial Application: From Protein Engineering to Bacterial Strains Mutation! A Comprehensive Review of Physical, Molecular, and Computational Approaches

Han,

Ullah,

Andoh

et al. 2024

J. Agric. Food Chem.

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Bacterial chitinases are integral in breaking down chitin, the natural polymer in crustacean and insect exoskeletons. Their increasing utilization across various sectors such as agriculture, waste management, biotechnology, food processing, and pharmaceutical industries highlights their significance as biocatalysts. The current review investigates various scientific strategies to maximize the efficiency and production of bacterial chitinases for industrial use. Our goal is to optimize the heterologous production process using physical, molecular, and computational tools. Physical methods focus on isolating, purifying, and characterizing chitinases from various sources to ensure optimal conditions for maximum enzyme activity. Molecular techniques involve gene cloning, site-directed mutation, and CRISPR-Cas9 gene editing as an approach for creating chitinases with improved catalytic activity, substrate specificity, and stability. Computational approaches use molecular modeling, docking, and simulation techniques to accurately predict enzyme–substrate interactions and enhance chitinase variants’ design. Integrating multidisciplinary strategies enables the development of highly efficient chitinases tailored for specific industrial applications. This review summarizes current knowledge and advances in chitinase engineering to serve as an indispensable guideline for researchers and industrialists seeking to optimize chitinase production for various uses.

show abstract

Isolation and identification of high-yielding alkaline phosphatase strain: a novel mutagenesis technique and optimization of fermentation conditions

Cited by 3 publications

References 45 publications

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Application of Atmospheric and Room-Temperature Plasma (ARTP) to Microbial Breeding

Isolation, Identification, and Fermentation Optimization of Phytase-Producing Bacteria and Their Effects on Soybean Seedlings

Engineering Bacterial Chitinases for Industrial Application: From Protein Engineering to Bacterial Strains Mutation! A Comprehensive Review of Physical, Molecular, and Computational Approaches

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