The present study was done to investigate the presence of keratinophilic fungi in soil, sixty soil samples were collected randomly from bovine farms in Khartoum state. Hair baiting technique was used for isolating the fungi. Plates which contain soil and children hair were then incubated at 28ºC for one month. Ten genera and fifteen species were isolated from cattle house soils. Aspergillus species represent the highest occurrence of isolates.