Isolation and Identification of l-Deoxy-l-(L-asparagino)-D-fructose Formed in the Autoclaved Culture Medium

Synthesis of 6-phosphofructose aspartic acid and some related Amadori compounds

“…[The ethylene glycol can be removed either by more thorough washing with water on the Dowex column or by extraction of the crude material with acetonitrile in a Soxhlet apparatus]. Compare the literature values of −40 7 and −60 8 , pH values unknown. Exact mass: calcd.…”

Fructose-Asparagine Is a Primary Nutrient during Growth of Salmonella in the Inflamed Intestine

“…Arginine is an intermediate case. Previous syntheses of F-Asn include those of Stadler et al [46], Wang et al [72], and Miura et al [73]. The procedure of Stadler et al [46] uses alkaline conditions which we thought could bring about isomerization of the sugar and racemization of the amino acid.…”

“…The 1 H-NMR spectrum is complex due to the equilibrating mixture of alpha- and beta- pyranose and furanose forms [55], but integration of the upfield resonances due to asparagine and the downfield resonances due to the sugar are in the proper ratio. The material was also characterized by its specific rotation and infrared (IR) spectrum: [α] 23 D −48° (c = 0.1, water) (reference [73] −40°, c = 1, water); IR (Nujol): 3350,3155, 1668, 1633, 1455, 1408, 1080 cm −1 . Compare our preparations to results in [71], [73].…”

“…The material was also characterized by its specific rotation and infrared (IR) spectrum: [α] 23 D −48° (c = 0.1, water) (reference [73] −40°, c = 1, water); IR (Nujol): 3350,3155, 1668, 1633, 1455, 1408, 1080 cm −1 . Compare our preparations to results in [71], [73].…”