No abstract

In the cultivation of red-colored yeast, Sporobolmny ces adorns AHU 3246, the growth of the yeast was stimulated when the synthetic medium was autoclaved for moderately long period. The analogous observa tion was also found between the autoclaved medium and the aseptically filtered medium. Such a phenomenon was generally found during the incubation of various microorganisms.1) Stimulation of initial growth of microorganisms was thought to be caused by the variation of components in the autoclaved culture solution. In connection with this heat activation effect many effective substances were identified to date, for example, the degradation products of sugars,2•`4) ascorbic acid5) glucosylglycine6) etc. The authors are interested in the formation of new nitrogen con taining compounds, especially ninhydrin positive sub stances in the autoclaved culture medium and this paper deals with isolation and identification of these compounds.