Isolation and identification of lactic acid bacteria from the intestinal tracts of honey bees, Apis mellifera L., in Egypt

Lactic acid bacteria isolated from the Stingless bee Scaptotrigona mexicana and partial characterization of their probiotic activity

“…morphologies of the strains found in this study correspond partially to the biota reported honey from Apis honey bees (Gilliam, 1997), which could come from the nectar collected (Gilliam, 1997) and/or could be of gastrointestinal origin (Elzeini et al, 2020). The presumed strains of LAB and probiotic potentials do not possess the enzymes catalase and oxidase, give violet coloration in the Gram stain, and grow in anaerobiosis (Kandler & Weiss, 1986).…”

“…The numbers of strains isolated were similar to those reported in the Scaptotrigona mexicana. Las morfologías macroscópicas de las cepas halladas en este estudio corresponden parcialmente con la biota reportada para miel de abejas melíferas (Gilliam, 1997), las cuales podrían provenir del néctar recolectado (Gilliam, 1997) y/o podrían ser de origen gastrointestinal de las abejas melíferas (Elzeini et al, 2020). Las presuntas cepas de LAB y potenciales probióticos no poseen las enzimas catalasa y oxidasa, dan coloración violeta en la tinción de Gram y crecen en anaerobiosis (Kandler & Weiss, 1986); criterios usados para seleccionar las 10 LAB.…”

Impact of Fermentation Processes on the Bioactive Profile and Health-Promoting Properties of Bee Bread, Mead and Honey Vinegar

“…Elzeini et al (2020) [8] isolated from the gut of A. mellifera L the following LAB: Lactobacillus brevis MH191230, Lactobacillus casei KT273339, and Enterococcus faecalis MG890204, KX073783, and EU594564, and evaluated their antibacterial activity against different types of bacteria with positive effects. The mechanisms of action by all LABs are elucidated by the production of several active compounds such as organic acids (acetic, lactic, oxalic, and glutaric acids).…”

“…), and Gram-negative bacteria (Pseudomonas spp., Achromobacter spp., Citrobacter spp., Enterobacter spp., Escherichia coli, Flavobacterium spp., and Klebsiella spp.) [8]. They play a key role in honey [9] and BB production [10,11], as well as in long-term stored food for larvae and adult honeybees.…”

Enterococcus faecium Regulates Honey Bee Developmental Genes

“…Among the probiotic bacteria used in honey bees, E. faecium is a generally commensal organism that is commonly found in animal gastrointestinal tracts, fermented food, dairy products, and in various environments including soil and water [13]. Enterococcus is common in honey bee colonies [14][15][16] and was first isolated from A. mellifera adults in 1972 [17]. E. faecium has been found in the gastrointestinal tract of the giant honey bee, A. dorsata [18] and in A. mellifera [19], confirming the ability of Enterococcus strains to persist in the intestinal tracts of other Apis genera.…”