Isolation and identification of lipopeptide antibiotics from Paenibacillus elgii B69 with inhibitory activity against methicillin-resistant Staphylococcus aureus

Ding, Rui; Wu, Ximei; Qian, Chao-Dong; Teng, Yuanwen; Ou, Li; Zhan, Zha‐Jun; Zhao, Yanfang

doi:10.1007/s12275-011-1153-7

Cited by 42 publications

(29 citation statements)

References 26 publications

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“…Polypeptins, octapeptins, pelgipeptins, gavaserin and saltavalin are LPs isolated from Paenibacillus sp. strains, reported before by other scientists, and should also be included in this collection [136, 152–155]. …”

Section: Resultsmentioning

confidence: 99%

Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species

2016

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BackgroundGram-positive bacteria of the Bacillales are important producers of antimicrobial compounds that might be utilized for medical, food or agricultural applications. Thanks to the wide availability of whole genome sequence data and the development of specific genome mining tools, novel antimicrobial compounds, either ribosomally- or non-ribosomally produced, of various Bacillales species can be predicted and classified. Here, we provide a classification scheme of known and putative antimicrobial compounds in the specific context of Bacillales species.ResultsWe identify and describe known and putative bacteriocins, non-ribosomally synthesized peptides (NRPs), polyketides (PKs) and other antimicrobials from 328 whole-genome sequenced strains of 57 species of Bacillales by using web based genome-mining prediction tools. We provide a classification scheme for these bacteriocins, update the findings of NRPs and PKs and investigate their characteristics and suitability for biocontrol by describing per class their genetic organization and structure. Moreover, we highlight the potential of several known and novel antimicrobials from various species of Bacillales.ConclusionsOur extended classification of antimicrobial compounds demonstrates that Bacillales provide a rich source of novel antimicrobials that can now readily be tapped experimentally, since many new gene clusters are identified.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3224-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species

2016

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“…[144]. Similarly, a new cyclic lipopeptide antibiotic from Pa. tianmuensis Battacin (Octapeptin B5) was reported active against multidrug-resistant Gram-negative bacteria [145].…”

Section: Lipopeptide Surfactants As Antibacterial Agentsmentioning

confidence: 98%

Lipopeptide surfactants: Production, recovery and pore forming capacity

2015

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“…The most frequent products are lipopeptides [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16], peptide/polyketide hybrids [17,18], as well as lantibiotics [19,20]. Such products qualify Paenibacillus strains for applications in agriculture, medicine, and biotechnology.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry

Vater

Niu

Dietel³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Paenibacillus polymyxa-M1 is a potent producer of bioactive compounds, such as lipopeptides, polyketides, and lantibiotics of biotechnological and medical interest. Genome sequencing revealed nine gene clusters for nonribosomal biosynthesis of such agents. Here we report on the investigation of the fusaricidins, a complex of cyclic lipopeptides containing 15-guanidino-3-hydroxypentadecanoic acid (GHPD) as fatty acid component by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). More than 20 variants of these compounds were detected and characterized in detail. Mass spectrometric sequence analysis was performed by MALDI-LIFT-TOF/TOF fragment analysis. The obtained product ion spectra show a specific processing in the fatty acid part. GHPD is cleaved between the α-and ß-position yielding two fragments a and b, one bearing the end-standing guanidine group and another one comprising the residual two C-atoms of GHPD with the attached peptide moiety. The complete sequence of all fusaricidins was derived from sets of b n -and y n -ions. The fusaricidin complex can be divided into four lipopeptide families, three of them showing variations of the amino acid in position 3, Val or Ile for the first and Tyr or Phe for families 2 and 3, respectively. A collection of novel fusaricidins was detected differing from those of families 1-3 by an additional residue of 71 Da (family 4). LIFT-TOF/TOF fragment spectra of these species imply that in their peptide moiety, an Ala-residue is attached by an ester bond to the free hydroxyl group of Thr 4 . More than 10 novel fusaricidins were characterized mass spectrometrically.

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Isolation and identification of lipopeptide antibiotics from Paenibacillus elgii B69 with inhibitory activity against methicillin-resistant Staphylococcus aureus

Cited by 42 publications

References 26 publications

Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species

Identification and classification of known and putative antimicrobial compounds produced by a wide variety of Bacillales species

Lipopeptide surfactants: Production, recovery and pore forming capacity

Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry

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