Isolation and Identification of Low‐Molecular‐Weight Peptides from Emmentaler Cheese

Combes, Christèle; Paterson, Eva; Amadò, Renato

doi:10.1111/j.1365-2621.2002.tb10637.x

Cited by 14 publications

(8 citation statements)

References 32 publications

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“…Peptidomics is used not only to study the digestion of proteins-it has many applications in dairy science. Dairy scientists employ peptidomics to identify peptides in dairy products, including cheeses [ 161 , 162 , 163 , 164 ], yoghurts [ 165 ], and kefir [ 150 ]. These peptidomics studies revealed that differences in starting materials, production techniques, and storage time resulted in major variation in the peptide profile.…”

Section: Advanced Analytical Techniques Of Milk and Dairy Productsmentioning

confidence: 99%

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

et al. 2020

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Food-based components represent major sources of functional bioactive compounds. Milk is a rich source of multiple bioactive peptides that not only help to fulfill consumers ‘nutritional requirements but also play a significant role in preventing several health disorders. Understanding the chemical composition of milk and its products is critical for producing consistent and high-quality dairy products and functional dairy ingredients. Over the last two decades, peptides have gained significant attention by scientific evidence for its beneficial health impacts besides their established nutrient value. Increasing awareness of essential milk proteins has facilitated the development of novel milk protein products that are progressively required for nutritional benefits. The need to better understand the beneficial effects of milk-protein derived peptides has, therefore, led to the development of analytical approaches for the isolation, separation and identification of bioactive peptides in complex dairy products. Continuous emphasis is on the biological function and nutritional characteristics of milk constituents using several powerful techniques, namely omics, model cell lines, gut microbiome analysis and imaging techniques. This review briefly describes the state-of-the-art approach of peptidomics and lipidomics profiling approaches for the identification and detection of milk-derived bioactive peptides while taking into account recent progress in their analysis and emphasizing the difficulty of analysis of these functional and endogenous peptides.

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Section: Advanced Analytical Techniques Of Milk and Dairy Productsmentioning

confidence: 99%

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

et al. 2020

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“…Peptidomic technologies have already been applied to identify hundreds of peptides from several cheeses, including Parmigiano–Reggiano , Emmental , Gouda , and cheddar . Peptidomics reveals that differences in cheese production starting materials, coagulation, and ripening result in major differences in peptides released across cheeses.…”

Section: Applications Of Peptidomicsmentioning

confidence: 99%

Current peptidomics: Applications, purification, identification, quantification, and functional analysis

et al. 2015

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Peptidomics is an emerging field branching from proteomics that targets endogenously produced protein fragments. Endogenous peptides are often functional within the body—and can be both beneficial and detrimental. This review covers the use of peptidomics in understanding digestion, and identifying functional peptides and biomarkers. Various techniques for peptide and glycopeptide extraction, both at analytical and preparative scales, and available options for peptide detection with MS are discussed. Current algorithms for peptide sequence determination, and both analytical and computational techniques for quantification are compared. Techniques for statistical analysis, sequence mapping, enzyme prediction, and peptide function, and structure prediction are explored.

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“…CZE was used for the determination of structural deviants of recombinant hirudin [208], for purity control of synthetic derivative of [8-D-Arg]-deamino vasopressin with methylated peptide bond between the second (Tyr) and third (Phe) amino acid residues [209], semisynthetic desoctapeptide insulin derivatives [210], for the analysis of four peptides, derived from the N-terminal fragment of a s1 -casein, isolated from a low-molecular-mass extract of a commercial Emmentaler cheese [211], for the purity tests of antioxidative deca-and pentadecapeptides separated from protein hydrolysate of lecithin-free egg yolk [212], and for the analysis of oligopeptides isolated from white wine [213]. For the full characterization of peptide preparations, especially pharmaceuticals and peptides used in biological tests, it is necessary to know also the content of low-molecular-mass ionic admixtures.…”

Section: Quality Control and Determination Of Puritymentioning

confidence: 99%

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Kasicka

2003

Electrophoresis

106

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An overview of the recent developments in the applications of high-performance capillary electromigration methods, namely zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography, to analysis, preparation, and physicochemical characterization of peptides is presented. New approaches to the theoretical description and experimental verification of the electromigration behavior of peptides and the methodological aspects of capillary electroseparations of peptides, such as rational selection of separation conditions, sample treatment, and suppression of adsorption, are discussed, and new developments in individual separation modes and new designs of detection systems applied to peptide separations are shown. Several types of applications of capillary electromigration methods to peptide analysis are presented: quality control and purity tests, determination in biomatrices, monitoring of physical and chemical changes and enzymatic conversions, amino acid and sequence analysis, and peptide mapping. The examples of micropreparative peptide separations are given and capabilities of capillary electromigration techniques to provide important physicochemical characteristics of peptides are demonstrated.

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Isolation and Identification of Low‐Molecular‐Weight Peptides from Emmentaler Cheese

Cited by 14 publications

References 32 publications

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Current peptidomics: Applications, purification, identification, quantification, and functional analysis

Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

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