Isolation and Identification of miRNAs in exosomes derived from serum of colon cancer patients

Zhao, Lei; Yu, Jing; Wang, Jing; Li, Huihui; Che, Juanjuan; Cao, Bangwei

doi:10.7150/jca.18026

Cited by 71 publications

(45 citation statements)

References 35 publications

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“…Recently, exosomes, and in particular neuronally derived exosomes, have been studied as potential biomarkers of NDs [ 99 – 101 ]. The promise of exosomes as biomarkers of pathology is due largely to their protein content and membrane composition because there is a high degree of variability in miRNA distribution across exosome populations, and contradictory data exist on the fraction of miRNAs circulating in exosomes as compared with other forms of cell-free miRNAs in the bloodstream [ 47 , 102 , 103 ].…”

Section: Discussionmentioning

confidence: 99%

Circulating brain-enriched microRNAs as novel biomarkers for detection and differentiation of neurodegenerative diseases

Sheinerman¹,

Toledo

Tsivinsky³

et al. 2017

Alz Res Therapy

149

166

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BackgroundMinimally invasive specific biomarkers of neurodegenerative diseases (NDs) would facilitate patient selection and disease progression monitoring. We describe the assessment of circulating brain-enriched microRNAs as potential biomarkers for Alzheimer’s disease (AD), frontotemporal dementia (FTD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS).MethodsIn this case-control study, the plasma samples were collected from 250 research participants with a clinical diagnosis of AD, FTD, PD, and ALS, as well as from age- and sex-matched control subjects (n = 50 for each group), recruited from 2003 to 2015 at the University of Pennsylvania Health System, including the Alzheimer’s Disease Center, the Parkinson’s Disease and Movement Disorders Center, the Frontotemporal Degeneration Center, and the Amyotrophic Lateral Sclerosis Clinic. Each group was randomly divided into training and confirmation sets of equal size. To evaluate the potential of circulating microRNAs enriched in specific brain regions affected by NDs and present in synapses as biomarkers of NDs, the levels of 37 brain-enriched and inflammation-associated microRNAs in the plasma of all participants were measured using individual qRT-PCR. A “microRNA pair” approach was used for data normalization.ResultsMicroRNA pairs and their combinations (classifiers) capable of differentiating NDs from control and from each other were defined using independently and jointly analyzed training and confirmation datasets. AD, PD, FTD, and ALS are differentiated from control with accuracy of 0.89, 0.90, 0.88, and 0.83 (AUCs, 0.96, 0.96, 0.94, and 0.93), respectively; NDs are differentiated from each other with accuracy ranging from 0.77 (AUC, 0.87) for AD vs. FTD to 0.93 (AUC, 0.98) for AD vs. ALS. The data further indicate sex dependence of some microRNA markers. The average increase in accuracy in distinguishing ND from control for all and male/female groups is 0.06; the largest increase is for ALS, from 0.83 for all participants to 0.92/0.98 for male/female participants.ConclusionsThe work presented here suggests the possibility of developing microRNA-based diagnostics for detection and differentiation of NDs. Larger multicenter clinical studies are needed to further evaluate circulating brain-enriched microRNAs as biomarkers for NDs and to investigate their association with other ND biomarkers in clinical trial settings.Electronic supplementary materialThe online version of this article (doi:10.1186/s13195-017-0316-0) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Circulating brain-enriched microRNAs as novel biomarkers for detection and differentiation of neurodegenerative diseases

Sheinerman¹,

Toledo

Tsivinsky³

et al. 2017

Alz Res Therapy

149

166

View full text Add to dashboard Cite

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“…The engineered CV‐1 cell membrane was derived with combined mechanical disruption and differential centrifugation . Then, a previously reported MB was synthesized to detect a model exosomal miRNA, miR‐21, which is a universal biomarker of many cancers including breast, lung, colon, and brain cancers . The MBs were mixed with the cell membrane and coextruded through porous polycarbonate membranes to generate the Vir‐FVs.…”

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Exosomal MicroRNAs Using Virus‐Mimicking Fusogenic Vesicles

et al. 2019

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Exosomal microRNAs (miRNAs) are important biomarkers for clinical diagnosis and disease treatment monitoring.However,m ost approaches for exosomal miRNA detection are time-consuming,l aborious,a nd expensive. Herein, we report av irus-mimicking fusogenic vesicle (Vir-FV) that enables rapid, efficient, and high-throughput detection of exosomal miRNAs within 2h.F usogenic proteins on Vir-FVs can specifically target the sialic-acid-containing receptors on exosomes,i nducing efficient fusion of Vir-FVs and exosomes.Upon vesicle content mixing, the molecular beacons encapsulated in Vir-FVs specifically hybridizew ith the target miRNAs in the exosomes,g enerating fluorescence.C ombined with flowcytometry,the Vir-FVs can not only detect exosomal miRNAs but also distinguish tumor exosomes from normal exosomes by sensing the tumor-related miRNAs,p aving the way towards the rapid and efficient detection of exosomal miRNAs for diagnosis and prognosis prediction of diseases.

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“…64 Furthermore, it was demonstrated that miR-21 is significantly overexpressed (five folds) in exosomes derived from patients with colon cancer, particularly in patients at the first diagnosis or before any treatments. 113 Generally, a unique miRNA like miR-21 is not an exact diagnostic marker in CRC; it requires to be accompanied with other miRNAs for improved specificity. 60 Another study in this area showed that exosomal miR-193a may be used as a biomarker for predicting the progression of colon cancer.…”

Section: Of Crcmentioning

confidence: 99%

Exosomes and their importance in metastasis, diagnosis, and therapy of colorectal cancer

Cheshomi

Matin

2018

J of Cellular Biochemistry

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Extracellular vesicles are known as actual intermediaries of intercellular communications, such as biological signals and cargo transfer between different cells. A variety of cells release the exosomes as nanovesicular bodies. Exosomes contain different compounds such as several types of nucleic acids and proteins. In this study, we focused on exosomes in colorectal cancer as good tools that can be involved in various cancer‐related processes. Furthermore, we summarize the advantages and disadvantages of exosome extraction methods and review related studies on the role of exosomes in colorectal cancer. Finally, we focus on reports available on relations between mesenchymal stem cell–derived exosomes and colorectal cancer. Several cancer‐related processes such as cancer progression, metastasis, and drug resistance of colorectal cancer are related to the cargoes of exosomes. A variety of molecules, especially proteins, microRNAs, and long noncoding RNAs, play important roles in these processes. The microenvironment features, such as hypoxia, also have very important effects on the properties of the origin cell–derived exosomes. On the other hand, exosomes derived from colorectal cancer cells also interfere with cancer chemoresistance. Furthermore, today it is known that exosomes and their contents can likely be very effective in noninvasive colorectal cancer diagnosis and therapy. Thus, exosomes, and especially their cargoes, play different key roles in various aspects of basic and clinical research related to both progression and therapy of colorectal cancer.

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Isolation and Identification of miRNAs in exosomes derived from serum of colon cancer patients

Cited by 71 publications

References 35 publications

Circulating brain-enriched microRNAs as novel biomarkers for detection and differentiation of neurodegenerative diseases

Circulating brain-enriched microRNAs as novel biomarkers for detection and differentiation of neurodegenerative diseases

Rapid Detection of Exosomal MicroRNAs Using Virus‐Mimicking Fusogenic Vesicles

Exosomes and their importance in metastasis, diagnosis, and therapy of colorectal cancer

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