Isolation and identification of Mycobacterium bovis in cattle slaughtered from an abattoir in Garanhuns, Pernambuco

Silva, Stephanie Caroline Gueiros; Alves, Amanda de Deus Ferreira; Almeida, Tássio José de Oliveira; Sá, Pamella Christhine Lins de; Filho, Antônio Francisco de Souza; Cárdenas, Nicolás Céspedes; Neto, José Soares Ferreira; Heinemann, Marcos Bryan; Azevêdo, Sérgio Santos de; Torres, Márcia Bersane Araújo de Medeiros

doi:10.5433/1679-0359.2018v39n1p157

Cited by 5 publications

(10 citation statements)

References 18 publications

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“…Bovine tuberculosis predominantly affects cattle and buffaloes, but may occasionally infect other mammalian species, including humans (Etchechoury et al, 2010;Silva et al, 2018). It may spread through direct contact with infected animals, causing the spread of disease among herds or herds to wild animals and vice versa (Corner, Murphy, & Gormley, 2011;Reis, 2015), or being transmitted through indirect contact with contaminated equipment, water and food (Palmer, 2013;Smith, Tauer, Schukken, Lu, & Grohn, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Carneiro

Pasquatti

Takatani³

et al. 2019

Veterinary Medicine & Sci

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The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Carneiro

Pasquatti

Takatani³

et al. 2019

Veterinary Medicine & Sci

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“…Different organs including lungs, lymph nodes, intestines, liver, spleen and heart have been used in previous abattoir surveys for BTB (Müller et al, 2008;Cardoso et al, 2009;Proano-Perez et al, 2011;Gizaw et al, 2017;Yahyaoui-Azami et al, 2017;Silva et al, 2018b). Among these organs, the highest number of BTB positive cases were associated with lung and lymph node lesions (Sa'idu et al, 2015b;Silva et al, 2018b). The presence of lung lesions had the strongest association with the detection of M. bovis infection by PCR (Sa'idu et al, 2015b;Silva et al, 2018b).…”

Section: Resultsmentioning

confidence: 99%

“…Among these organs, the highest number of BTB positive cases were associated with lung and lymph node lesions (Sa'idu et al, 2015b;Silva et al, 2018b). The presence of lung lesions had the strongest association with the detection of M. bovis infection by PCR (Sa'idu et al, 2015b;Silva et al, 2018b). Cranial lung lobes are commonly associated with pulmonary lesions of BTB (Menin et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Even though the carcasses from healthy cattle without visible tuberculous lesions can be certified as fit for human consumption, M. bovis DNA can be detected in infected animal tissue well before the appearance of gross lesions (Swift et al, 2016). Therefore, inclusion of molecular detection methods may increase the sensitivity of abattoir monitoring for BTB (Proano-Perez et al, 2011;Sa'idu et al, 2015b;Silva et al, 2018b).…”

Section: March 2021mentioning

confidence: 99%

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Detection of Mycobacterium bovis in cattle lungs from two abattoirs in Western and North Central provinces of Sri Lanka

Jayasumana

Dunuwila

Palkumbura

et al. 2021

J. Natn. Sci. Foundation Sri Lanka

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Mycobacterium bovis causes bovine tuberculosis (BTB) primarily in cattle. All mammals are susceptible to this zoonotic disease and it had been reported from the Central and North Western provinces of Sri Lanka. Abattoir monitoring is usually used for BTB surveillance in endemic countries. However, proper ante-mortem inspection of cattle and proper meat inspection is practiced only at a few abattoirs in Sri Lanka. The objective of this study was to conduct a preliminary assessment of BTB incidence among cattle used for beef production at two abattoirs in Western (WP) and North Central (NCP) provinces in Sri Lanka. Randomly collected 115 lung samples (WP = 45; NCP = 70) were tested using direct acid-fast staining, culture on Lowenstein Jensen medium, histopathology and PCR. Mycobacterium tuberculosis complex was detected in 7.0 % of the samples by PCR conducted using DNA extracted directly from samples. Only 5.2 % of the samples (4.45 % from WP and 5.7 % from NCP) were positive for M. bovis specific PCR. Only one (0.87 %) PCR positive sample from NCP had a granuloma on histopathological observation, suggesting relatively low incidence of BTB among the cattle processed at these abattoirs. Mycobacterium bovis isolates were not recovered and acid-fast bacilli were not observed in direct smears. Initiating proper meat inspection at all abattoirs in the country along with increased BTB surveillance capacity of the national veterinary service are required to mitigate this risk. Further studies are essential to determine the exact prevalence of BTB in Sri Lanka and to identify any wildlife reservoirs of BTB in the country.

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“…A reduced number of M. bovis isolates have also been observed in studies that bacteriologically evaluated both lesions suggestive of BTB and nonspecific lymphadenitis during postmortem examination (Furlanetto et al 2012, Alzamora Filho et al 2014a. Considered the gold standard for BTB diagnosis, higher isolation rates are expected in bacteriological culture, with mycobacterial growth rates that can vary from 60.0 (Nassar et al 2007) to 68.75% (Silva et al 2018) of the suggestive lesions. In contrast, despite the low frequency of positive samples verified in the present study, ZN staining revealed AARB + in 100% of the isolates, confirming the high identification specificity of the mycobacterial method (Corner 1994).…”

Section: Discussionmentioning

confidence: 99%

Isolation and genotyping of Mycobacterium bovis in suggestive lesions of tuberculosis in cattle slaughtered in the state of Ceará, Brazil

et al. 2020

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Bovine tuberculosis (BTB) is a zoonosis caused by the bacterium Mycobacterium bovis, which induces the development of nodular and granulomatous lesions in various animal tissues. The recognition of these suggestive gross lesions during postmortem sanitary inspection in slaughterhouses provides a presumptive diagnosis, which requires the use of complementary tests to confirm the disease. This study aimed to verify the occurrence of BTB in cattle slaughtered in slaughterhouses in the state of Ceará, Brazil, using bacteriological and molecular methods. To this end, suggestive lesions were analyzed on carcasses condemned by the “Serviço de Inspeção Estadual” (SIE). The samples were submitted to microbiological analysis using culture media and specific staining followed by spoligotyping molecular technique for identification and genotyping of the mycobacteria. Occurrence of lesions suggestive of BTB was verified in bovine carcasses (0.071%) from different municipalities of the state. These lesions were located mainly in the lung (95.12%), lymph nodes (58.53%), and liver (36.58%). Microbiological culture showed bacterial isolation (17.94%), with the growth of colonies showing morphological and tannic characteristics belonging to genus Mycobacterium spp. Genetic polymorphism analysis identified M. bovis in all isolates, which were discriminated into six spoligotypes (SB0121, SB0295, SB1064, SB0120, SB0870, and SB0852). These profiles have been described in Brazil and several areas of the world, except for profiles SB1064 and SB0852, which were described in the country for the first time. The results show that the association of the diagnostic methods used was the basis for the first study on identification of mycobacteria found in the state, which may provide a database for the epidemiological study of BTB in the state of Ceará.

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Isolation and identification of Mycobacterium bovis in cattle slaughtered from an abattoir in Garanhuns, Pernambuco

Cited by 5 publications

References 18 publications

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Detection of Mycobacterium bovis in cattle lungs from two abattoirs in Western and North Central provinces of Sri Lanka

Isolation and genotyping of Mycobacterium bovis in suggestive lesions of tuberculosis in cattle slaughtered in the state of Ceará, Brazil

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