Tuberculosis is a chronic infectious disease caused by the Mycobacterium tuberculosis complex (CMT), members including M. bovis. The sanitary inspection in slaughterhouses has a great importance on public health for the removal of contaminated meat with pathological lesions. Cattle slaughtered in the abattoir of Garanhuns were subjected to macroscopic, histological, bacteriological, and molecular analyses. Lung inspection revealed gross lesions suggestive of tuberculosis. The characterization of tuberculous granulomas was performed by histopathology and hematoxylin and eosin (HE) staining. The identification of acid-fast bacilli (AFB) was performed on smears with fresh material and histological staining using Ziehl-Neelsen stain. Bacteriological diagnosis was carried out using Stonebrink and Lowenstein-Jensen media, and polymerase chain reaction (PCR) was performed from the DNA extracted from colonies for the identification of Mycobacterium spp. We found that 1% (32/3,.180) of the collected lungs showed lesions suggestive of tuberculosis. Histopathological analysis revealed that 65.62% (21/32) samples had granulomatous pneumonia. AFB was detected in 46.88% (15/32) smears and 4.16% (1/24) histological sections, as analyzed by Ziehl-Neelsen staining. Moreover, 68.75% (22/32) samples showed bacterial growth and molecular identification 90% (18/20) of the samples were positive for M. bovis and 10% (2/20) for Mycobacterium sp. Thus, microbiological culture and PCR analyses revealed the high rate of bovine tuberculosis in this region, indicative of the need to disclose these results in the interest of public health. Consequently, we emphasize the need for an accurate macroscopic evaluation of lesions suggestive of bovine tuberculosis. Key words: Granuloma. Pneumonia. Public health. ResumoA tuberculose é uma doença infecciosa crônica causada por membros do complexo Mycobacterium tuberculosis (CMT), incluindo M. bovis. A inspeção sanitária em matadouros frigoríficos possui grande importância para a saúde pública pela remoção de carne contaminada com lesões patológicas. Os bovinos abatidos no matadouro de Garanhuns-PE foram submetidos a análises macroscópicas, histológicas, bacteriológicas e moleculares. A inspeção pulmonar revelou lesões sugestivas de tuberculose.A caracterização de granulomas tuberculoides foi realizada através da histopatologia e coloração de hematoxilina e eosina (HE). A identificação de bacilo álcool -ácido resistente (BAAR) foi realizado em esfregaços com material fresco e em cortes histológicos sob a coloração de Zihel-Neelsen. O diagnóstico bacteriológico foi realizado em meio de cultivo Stonebrink e Lowenstein-Jensen e a reação em cadeia da polimerase (PCR) sucedeu a partir de DNA extraído de colônias para identificação do Mycobacterium sp... Verificou-se que1% (32/3.180) dos pulmões coletados apresentaram lesões sugestivas de tuberculose. Na análise histopatológica 65.62% (21/32) amostras tinham pneumonia granulomatosa. BAAR foram detectados em 46,88% (15/32) esfregaços e 4,16% (1/24) ...

RESUMO: O Carcinoma de Células Escamosas é um tumor maligno dos queratinócitos.No equino representa de 6-10% de todas as neoplasias que afetam a genitália externa. Os sinais geralmente incluem placas despigmentadas, irregularidades da superfície do pênis ou prepúcio, erosões incuráveis com ou sem tecido de granulação acompanhado e casos de metástase são observados em aproximadamente 18% das ocorrências. Microscopicamente observa-se formação de pérolas de queratina, mitoses e atipia celular. O diagnóstico é feito a partir dos sinais clínicos, achados histopatológicos, ultrassonografia e punção aspirativa. Relata-se um caso de um equino, macho, castrado, 13 anos, SRD, pelagem alazão, que foi atendido no Hospital Veterinário do Campus Palotina da Universidade Federal do Paraná. Devido ao mau estado corporal o animal foi submetido à eutanásia, sendo realizada a necropsia e exame histopatológico dos órgãos que foram corados pela coloração de Hematoxilina e Eosina. Na necropsia foi observada uma massa irregular ulcerada, firme, branco-amarelada e com superfície de aspecto granular no pênis e prepúcio. Os pulmões indicaram presença de metástase. No histopatológico confirmou-se que as lesões eram metástases do carcinoma de células escamosas. O objetivo deste trabalho é relatar um caso de carcinoma de células escamosas em pênis e prepúcio de equino com metástase pulmonar. Palavras-chave: histopatológico, neoplasia, pênisABSTRACT: Squamous Cell Carcinoma is a malignant tumor of keratinocytes. In the horse represents 6-10% of all cancers affecting the external genitalia. The signals typically include unpigmented plaques, surface irregularities of the penis or the prepuce, incurable with or without erosions accompanied by granulation tissue and cases of metastasis are observed in approximately 18% of cases. Microscopically observed formation of keratin, mitoses and cellular atypia. Diagnosis is made from clinical signs, histopathological findings, ultrasound and needle aspiration. We report a case of a horse, male, neutered, 13 years, SRD, sorrel coat, which was the Veterinary Hospital of Palotina Campus of Federal University of Paraná. Due to poor body condition the animal was euthanized, necropsy and histopathological examination of organs which were stained with hematoxylin and eosin staining being held. At necropsy an ulcerated, firm, white-yellowish and granular appearance on the surface of the penis and foreskin irregular mass was observed. The lungs showed metastasis. In the histopathological it was confirmed that the lesions were metastases, squamous cell carcinoma. The objective of this study is to report a case of squamous cell carcinoma of the penis and foreskin in equine pulmonary metastasis.

The aim of this study was to evaluate the main nonconformities that result in cytopathological samples inappropriate for diagnosis in veterinary medicine. All cytopathological samples, obtained from different canine and feline tissues/lesions, included in the study were received and classified as inconclusive by a single public laboratory of veterinary pathology, located in Pernambuco State, Brazil, between 2012 and 2016. Nonconformities regarding the smear quality, cellularity, presence or absence of hemorrhage, cellular overlapping, desiccation, and presence or absence of necrotic debris and/or artifacts were evaluated. Data were tabulated using Microsoft Excel 2007; absolute and relative frequencies were calculated using EPIINFO 3.5.2. From the 3268 cases received between 2012 and 2016, 190 cases were selected and comprised 514 inconclusive slides. The most frequent nonconformities detected were insufficient/absence of cellularity in 100% (514/514), inadequate submacroscopic presentation in 87% (446/514), and hemorrhage in 69% (356/514) of samples. Other features identified were cellular overlapping in 34% (175/514), inadequate staining in 31% (175/514), artifacts in 30% (154/514), desiccation in 28% (145/514), and necrotic debris in 26% (133/514) of samples. The implementation of laboratory standard operational procedures aimed at maintaining quality is essential. It is necessary to initially identify the main errors occurring in the processing stages as a way to guide and design strategies to avoid them.

Os ependimomas são neoplasias benignas ou malignas de células ependimárias que revestem o plexo coroide, o assoalho dos ventrículos cerebrais e o canal medular. São raros em animais domésticos, mas há relatos em cães e gatos. Neste relato descreve-se um carcinoma metastático de células ependimárias diagnosticado em equino Mangalarga Marchador, com morte decorrente de choque circulatório. Nódulos de superfície levemente rugosa, firme e coloração parda presentes no plexo coroide do IV ventrículo, rim e pulmão foram coletados, fixados em solução de formalina tamponada a 10%. Os fragmentos foram processados com inclusão em parafina e coloração em Hematoxilina/Eosina (HE) e imunomarcados para detecção de GFAP, vimentina e AE1/AE3. Os nódulos do IV ventrículo continham células ependimárias com anisocitose, anisocariose e figuras de mitose. Este padrão celular foi observado no rim e pulmão. Na imunohistoquímica, o nódulo do IV ventrículo foi positivo para GFAP, negativo para vimentina e fortemente positivo para AE1/AE3. No rim e pulmão os fragmentos foram positivos apenas para AE1/AE3. A descrição histopatológica e imunohistoquímica confirmaram diagnóstico do tumor de células ependimárias no IV ventrículo encefálico com metástase renal e pulmonar. Este tumor é considerado raro na espécie equina.