Isolation and Identification of Ornithobacterium rhinotracheale from Commercial Broiler Flocks on the Delmarva Peninsula

Odor, E. M.; Salem, M.; Pope, Conrad R.; Sample, Brenda F.; Primm, M; Vance, Kristen; Murphy, Molly D.

doi:10.2307/1592468

Cited by 34 publications

(21 citation statements)

References 2 publications

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“…At the beginning of the disease (in younger chickens) the exudate was foamy, whitish-yellow, similar to yogurt. Similar finding was described characteristic for O. rhinotracheale infection by some authors (Odor et al, 1997;van den Bosch, 2001;Gornatti Churria et al, 2011). In the air sacs of infected broilers can be seen a foamy, white, yogurt-like exudate, commonly accompanied with unilateral pneumonia.…”

Section: Patomorphological Analysis For Control Flockssupporting

confidence: 87%

Examination of Ornithobacterium rhinotracheale presence and pathomorphological changes in broiler respiratory organs in intensive broiler production

Gavrilović¹,

Jovanović²,

Živulj³

2012

ACTA VET-BEOGRAD

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Three flocks of broilers from the epizootiological region of South Banat, Serbia were chosen for examination, one flock with manifestation of respiratory disorders and two control flocks without respiratory disorders. In the flock with manifested respiratory disorders which was marked as flock number one (flock 1), high seroprevalence of specific antibodies for O. rhinotracheale (46%) was found in 30-dayold broiler chickens. The symptoms presented were: depression, reduction in feed consumption and water intake, sneezing, mucosal nasal discharge and facial edema. Production results were below technological standards due to increased mortality, decreased growth range and increased number of stunted chickens. Autopsy found frequent changes in the trachea, air sacs and lungs, which were manifested as: tracheitis catarrhalis, aerosacculitis fibrinosa and pneumonia fibrinosa. In control flocks marked as flock number two (flock 2) and flock number three (flock 3) where 4 % and 8 % of seropositive chickens were found, there were no clinical signs which would indicate illness, and production results were much closer to technological standards as compared with flock 1. Autopsy found lower number of chickens with changed respiratory organs as compared with flock 1; in addition intensity of changes was lower than in flock 1. No case of airsacculitis was found in flocks 2 and 3, which was a frequent finding in flock 1

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Section: Patomorphological Analysis For Control Flockssupporting

confidence: 87%

Examination of Ornithobacterium rhinotracheale presence and pathomorphological changes in broiler respiratory organs in intensive broiler production

Gavrilović¹,

Jovanović²,

Živulj³

2012

ACTA VET-BEOGRAD

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“…Cultivation and morphological characteristics of O. rhinotracheale WT isolates and SCVs. The best results for isolation of O. rhinotracheale were achieved by picking pinpoint colonies on SBA (with or without gentamicin) after incubation for 24 h at 37°C under microaerobic conditions (39). Furthermore, unique characteristics of the O. rhinotracheale colonies were their paraffinic structure and their poor adherence to agar (40).…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

et al. 2013

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bOrnithobacterium rhinotracheale is a Gram-negative bacterium associated with respiratory diseases in many avian species, with worldwide distribution, and it causes significant economic loss to the poultry industry. In this study, the isolation and characterization of O. rhinotracheale small-colony variants (SCVs) are described for the first time. O. rhinotracheale isolates (n ‫؍‬ 27) were recovered from tracheal samples (n ‫؍‬ 321) collected from different avian species with clinical signs of respiratory disease. Of the 27 O. rhinotracheale isolates, 21 (77.8%) showed SCVs in their primary cultures. Five O. rhinotracheale SCV isolates showed high levels of stability and were chosen for further characterization with their wildtype (WT) isolates. Stable O. rhinotracheale SCVs were oxidase negative, while their WT isolates were positive. Growth curves for stable O. rhinotracheale SCVs indicated lower growth rates and longer lag phases than for their WT isolates. Furthermore, it was possible to increase the efficacy of the broth medium in supporting the growth of O. rhinotracheale WT isolates by supplementing it with 5% fetal bovine serum (FBS) and 2% IsoVitaleX Enrichment. Antibiotic susceptibility tests showed that O. rhinotracheale SCVs had higher MIC values than their WT isolates. This study suggests that successful antibiotic treatment of respiratory diseases associated with O. rhinotracheale must take into consideration the resistance patterns of O. rhinotracheale SCVs. Intracellular persistence in murine RAW 264.7 macrophages revealed that O. rhinotracheale SCV28 had higher survival rates than its WT isolate. Finally, small-colony variants may be important contributors to the pathogenesis of O. rhinotracheale.

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“…The plates were incubated at 37°C under microaerobic conditions (5-10% CO 2 ) as well as at 37°C under aerobic conditions for 48 hours. The biochemical identification was carried out as described previously (Odor et al 1997;van Empel et al 1997) using API 20NE kit (Bio Merieux, Marcy, France).…”

Section: Bacteriological Examinationsmentioning

confidence: 99%

Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran

Ghanbarpour

Salehi

2009

Trop Anim Health Prod

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Ornithobacterium rhinotracheale is a gram negative bacterial pathogen causing respiratory tract infections in poultry. Tracheal, lung and serum samples were obtained from 21 broiler flocks of 8 farms from a slaughterhouse located in south-eastern of Iran. Among 630 tracheal and lung samples from samples resulting from 315 chickens, 11 (3.5%) ORT isolates were identified using biochemical tests. The isolates originated from 9 (42.9%) flocks out of 4 farms. All of the isolates were recovered from tracheal swabs and showed an API 20NE identification biocode 0-2-2-0-0-0-4. Of the 420 serum samples examined by ELISA, 134 (31.9%) sera from 17 (81.0%) flocks were positive for ORT antibodies. These results indicate that ORT is present in most broiler flocks with respiratory disorders in southeast Iran.

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Isolation and Identification of Ornithobacterium rhinotracheale from Commercial Broiler Flocks on the Delmarva Peninsula

Cited by 34 publications

References 2 publications

Examination of Ornithobacterium rhinotracheale presence and pathomorphological changes in broiler respiratory organs in intensive broiler production

Examination of Ornithobacterium rhinotracheale presence and pathomorphological changes in broiler respiratory organs in intensive broiler production

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

Sero-prevalence and identification of Ornithobacterium rhinotracheale in broiler flocks in south-eastern Iran

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