Isolation and Identification of Salmonella Spp. From Poultry Farms by Using Different Techniques and Evaluation of Their Antimicrobial Susceptibilities

Abstract

This study aimed to isolate and identify Salmonella spp. from various sources of poultry farms by using four different techniques (conventional biochemical tests, API 20E system, serology and polymerase chain reaction) the total number of isolates was 44(9%). The Salmonellae including 4 species, S. gallinarum 9(1.85%), S. typhimurium 7(1.44%), S. newport 21(4.3%) and S. ohio 1(0.21%). The highest isolation rate was in first week of chicks life 18(25.7%), however, the highest isolation rate of salmonella was from liver 13(28.8%). There are similarity in identification rate of Salmonella spp. between API 20 E system and PCR assay using flic gene. In this study using PCR amplification of rfbsg and rfbsp genes in differentiation of Salmonella serovar gallinarum into S. gallinarum and S. pullorum biovars very useful. Results of antimicrobial susceptibility revealed high resistance of isolates against seven antibiotics arranged in descending from high to low resistance (Azithromycin, Florfenicol, Trimethoprime-sulphamethaxezole, Tetracycline, Ciprofloxacin, Ampicillin and Gentamycin).

Molecular Detection of some Antimicrobial Resistance Genes in Salmonella Species Isolated from Commercial Layers in Egypt

“…The prevalence of the isolated bacteria from different internal organs of the examined layers in this study showed that the highest percentage of Salmonella was recorded in liver (37.5%) followed by cecum (33,3%), ovary (20.8%) and heart (8.3%). These results are in agreement with Dhahar et al [22] and AL-Iedani [23]. AL-Abadi and Al-Mayah [20] recorded that the highest percentage of Salmonella isolation was from cecum which is the primary sites of colonization of Salmonella due to the anatomical and structural location that allows the cecum to act as a blind sac with low content flow rate.…”

Isolation and characterization of outer membrane proteins (OMPs) from Salmonella Gallinarum in chicken and antibiogram of the isolates

“…Also a same study was carried out by Desai et al (2005) for the serotype-specific detection of Pullorum and its differentiation from the closely related Gallinarum using a rapid allele-specific polymerase chain reaction (PCR) method based on the nucleotide polymorphism in rfbS gene sequence. This observation also confirms the preliminary findings made by Park et al (2001) in Korea for differential diagnosis of S. Gallinarum and S. Pullorum using PCR-RFLP and AL-Iedani et al (2014) using rfbsg and rfbsp genes amplification of S. gallinarum and S. pullorum . Salmonella possess surface structures that can induce protective humoral and cellular immune responses following experimental infection in poultry (Liu et al, 2001;Timms et al, 1994).…”

Effect of Age and Season on Salmonella Infection in Broiler Chickens with Special Reference to Virulence and Antibiotic Resistance Genes

“…They are inexpensive antibiotics, which have been used extensively in the prophylaxis and therapy of human and animal infections and also at subtherapeutic levels in animal feed as growth promoters. [42], in the present study chicken showing high resistance to be recorded against Tetracycline, [43,44]. Higher rates of resistance were observed to extend spectrum penicillin, these antimicrobial resistances of Salmonella spp.…”