Isolation and Identification of Salmonella enteritidis using bacteriological and molecular technique from calves with diarrhea in Diwanihyia city

Abstract

Salmonella enteritidis is an important foodborne pathogen that affects the human health by conception animal products and it was considered as a worldwide public health hazard. This study was conduct to investigated the prevalence of S. enteritidis, in calves with diarrhea in farm of AL- Diwanihyia city basis of molecular properties of this bacterium . A total of 91 samples were collected from December 2013 to May 2014, in different farms in the al Diwanihyia city . the pathogen was isolated by using microbiological and biochemical tests. the DNA extraction was done by genomic DNA kit according to the manufacturer’s instruction (USA) and PCR was performed via the specific primers of SefA- -F and SeA-R of the SefA gene. Amplified fragments of the 210 bp were observed in 12 of the total 91 stool samples isolates. This study recommends that the identification of these pathogens by PCR technique can be replaced with traditional bacteriological tools. The PCR method is a rapid approach for recognizing and identifying the S. enteritidis infections in diarrheic calves.