Isolation and Identification of Staphylococcus aureus from Dairy Farms in Bishoftu Town, Ethiopia

Duguma, Ararsa

doi:10.19080/jojph.2018.03.555604

Cited by 2 publications

(2 citation statements)

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“…Several studies showed prevalence of Staphylococcus aureus among dairy cows with SCM at variable levels, viz. 42.85% (Ararsa et al 2018), 36.9% (Wubish et al 2013), 31.19% (Tadesse and Chanie 2012) and 21% (Barrett et al 2005).…”

Section: Resultsmentioning

confidence: 98%

“…Of the several microbial mastitogens, Staphylococcus aureus appears to be the most prevalent organism of public health significance worldwide (Kubota et al 2007). It is associated with contagious mastitis in bovines and is shed in milk thereby acting as source of infection to other mates in the shed (Radostits et al 2007, Ararsa 2018. Several studies showed prevalence of Staphylococcus aureus among dairy cows with SCM at variable levels, viz.…”

Section: Resultsmentioning

confidence: 99%

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Field level interventions on subclinical mastitis and detection of Staphylococcus in crossbred dairy cows

Karabasanavar¹,

Radder²,

Sivaraman

2019

Indian J of Anim Sci

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Mastitis has emerged as one of the major managemental diseases of economic importance of high yielding dairy cows. A cross sectional study was carried out on 116 cross-bred dairy cows of small scale dairy farms; subclinical mastitis (SCM) was detected in 22 animals (cow-wise prevalence, 18.96%). Of the 88 individual quarterâ€™s milk tested using California mastitis test (CMT) and somatic cell count (SCC) tests, 51 quarters showed SCM (quarterwise prevalence, 57.9%). Quarter-wise prevalence of SCM was 29.4, 31.4, 23.5 and 15.7% in right-fore (RF), right-hind (RH), left-fore (LF) and left-hind (LH) quarters, respectively. Fore-(52.9%) and right-(60.8%) quarters showed higher prevalence of SCM than hind-(47.1%) and left-(39.2%) quarters. All 4 quarters were found affected with SCM in 47% cows followed by 3-(23.5%), 1-(17.6%) and 2-(11.8%) quarters. Staphylococci were isolated and confirmed using 16S rRNA gene based genus-specific PCR in 39.2% of SCM affected quarters. Virulence associated nuc gene was detected in 75% of Staphylococcus isolates indicating their potential pathogenicity. Antibiogram showed multiple drug resistance (â‰¥3 antimicrobial category) in 63.6% of Staphylococci. Multiple antimicrobial resistance (MAR) was recorded in 31.8% isolates. However, none of the isolate carried mecA gene. Interventions, viz. clean milk production practices, antimicrobial therapy and non-specific supportive treatments resulted in 77.7, 50 and 38.8% reductions in SCM compared to the untreated control (37.5%). This study accentuated higher prevalence of SCM among dairy cattle and predominance of Staphylococcus as the major mastitogen. Early detection and management of SCM among dairy cattle is recommended so as to prevent its progression to clinical illness and curtail potential economic loss to farmers.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Field level interventions on subclinical mastitis and detection of Staphylococcus in crossbred dairy cows

Karabasanavar¹,

Radder²,

Sivaraman

2019

Indian J of Anim Sci

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Prevalence and Phage-Based Biocontrol of Methicillin-Resistant Staphylococcus aureus Isolated from Raw Milk of Cows with Subclinical Mastitis in Vietnam

Son,

Duc

2024

Antibiotics

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S. aureus, particularly methicillin-resistant S. aureus, has been recognized as a main cause of bovine mastitis and food poisoning. This study investigated the prevalence, antibiotic resistance, and phage-based biocontrol of S. aureus and methicillin-resistant S. aureus isolated from raw milk of cows with subclinical mastitis. The results showed that the prevalence of S. aureus and methicillin-resistant S. aureus was 12% (48/400) and 1.5% (6/400), respectively. The S. aureus isolates were highly resistant to penicillin (72.92%), erythromycin (43.75%), and tetracycline (39.58%). Out of 48 S. aureus isolates, 6 were identified as methicillin-resistant strains. Among them, one isolate was found to harbor the sea gene. A total of 5 phages were recovered from 50 pork and 50 chicken meat samples, 1 from pork and 4 from chicken meat samples. Phage PSA2 capable of lysing all 6 methicillin-resistant isolates was selected for characterization. The use of phage PSA2 completely inactivated methicillin-resistant S. aureus SA33 in raw milk at both 24 °C and 4 °C, indicating its potential as a promising antibacterial agent in controlling methicillin-resistant S. aureus in raw milk and treating bovine mastitis.

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Isolation and Identification of Staphylococcus aureus from Dairy Farms in Bishoftu Town, Ethiopia

Cited by 2 publications

References 6 publications

Field level interventions on subclinical mastitis and detection of Staphylococcus in crossbred dairy cows

Field level interventions on subclinical mastitis and detection of Staphylococcus in crossbred dairy cows

Prevalence and Phage-Based Biocontrol of Methicillin-Resistant Staphylococcus aureus Isolated from Raw Milk of Cows with Subclinical Mastitis in Vietnam

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