Isolation and identification of the antibacterial compounds in Coptis chinensis for the preservation of wood

Abstract

Wood is a biomass material that is easily eroded by wood-rotting fungi. Coptis chinensis is a natural green plant, which has an inhibitory effect on most microorganisms. Based on the highly toxic effects of the currently used wood chemical preservatives on humans, animals, and the environment, Coptis chinensis was selected to perform decay resistance experiments of wood in this paper. The active ingredients with bacteriostatic properties in Coptis chinensis were separated and screened via chemical treatment, and their structure was identified via nuclear magnetic resonance spectroscopy. The primary bacteriostatic components in Coptis chinensis were berberine hydrochloride, palmatine, and jatrorrhizine. The bacteriostatic zone experiment with a single component and different compounds for white-rot and brown-rot fungus were tested by the disc agar diffusion method. The bacteriostatic effect of berberine hydrochloride in a single active fraction was better. The three-fraction compound had the best bacteriostatic effect and was equivalent to alkaline copper quaternary. The natural active bacteriostatic fractions in Coptis chinensis had noticeable inhibitory effects on white-rot fungus (Trametes versicolor (L.) Lloyd) and brown-rot fungus (Gloeophyllum trabeum (Pers.) Murrill). The minimum bacteriostatic concentration was 0.01 g/mL. The results showed that Coptis extracts had potential as a wood protectant.

Antifungal Potential of Some Herb Decoctions and Essential Oils on Candida Species

“…Antibacterial compounds of C. chinensis have been isolated, identified and tested for the preservation of wood, showing favorable results with extracts in various solvents with better outcomes when isolated compounds acted in synergy [ 29 ], which can help to understand the identical results achieved by both C. chinensis decoction and EO in our study. Moreover, the results obtained in our study go hand in hand with those of Seneviratne et al [ 8 ] when it comes to the antifungal action of rhizome C. chinensis in C. krusei, C. glabrata , and C. tropicalis , and diverge for C. albicans and C. parapsilosis , where the authors reported no antifungal activity.…”

“…Looking closer at the similarities between their susceptibility testing results, it is possible to deduce that compounds responsible for the antifungal activity are extracted in an identical way involving temperature rising, either by the simmering in the decoction or by the steam distillation of the EO. According to previous studies, several bioactive components, including isoquinoline alkaloids such as berberine hydrochloride ( Figure 1 ) and palmatine hydrochloride ( Figure 2 )—both with methoxy groups in their chemical structure—have already been identified in the C. chinensis rhizome [ 29 ] and proved positive antimicrobial activity [ 30 ].…”

Oxyepiberberine from the Active Site of Coptidis Rhizoma Inhibits Lung Adenocarcinoma by Regulating SRC-related Pathways in vivo

“…Berberine found in HL has the capability to attain an anti-tumor effect through regulating autophagy, eliminating tumor cells, enhancing sensitivity and efficiency, reversing drug resistance, blocking tumor cell invasion and metastasis, impeding tumor angiogenesis, regulating cell cycle, inducing cell death, scavenging and inhibiting free radicals, as well as other mechanisms (Liu et al, 2019; Su et al, 2017; Wang et al, 2015). Furthermore, HL is recognized as a “Traditional Chinese Medicine (TCM) antibiotic”, attributed to its anti-bacterial and anti-inflammatory properties (Hao et al, 2020; Wang et al, 2021).…”