Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis

Fujishiro, Kinya; Ota, Toshio; Hasegawa, Mamoru; Yamaguchi, Kazuo; Mizukami, Toru; Uwajima, Takayuki

doi:10.1016/0006-291x(90)90734-5

Cited by 28 publications

(19 citation statements)

References 8 publications

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“…8) DNA sequence analysis confirmed that the 2.4-kb MluI fragment of pSca8 contained the entire structural Mlul Sphl I J gene of choB together with the upstream 616-bp of the 5'-flanking sequence. 9 ) Then, we examined the possibility of the expression of choB by external transcription read through from the lac promoter.…”

Section: Expression Of Chob By the Lac Promoter In E Colimentioning

confidence: 76%

“…Construction of pSca8 containing choB was previously described. 8 ) Generation of the deletion fragments. A 2.4-kb MluI fragment of pSca8, which contained choB with 616-bp of the 5'-flanking region, was blunt-ended by T4 DNA polymerase and introduced into the SmaI site of pUCl19 (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The assay conditions of the enzyme activities were previously described. 8 ) Purification of the extracellular cholesterol oxidase from S. lividans TK23(pCHS31). S. lividans TK23(pCHS31) was grown at 30°C for 150h in SK No.…”

Section: Construction Of P Lasm Ids For the Expression Of Chob In Smentioning

confidence: 99%

“…Brevibacterium sterolicum A TCC 21387 produces cholesterol oxidase in the culture filtrate. 2) We previously cloned the gene (choB) 8) and analyzed the nucleotide (nt) sequence.…”

mentioning

confidence: 99%

“…8) One purpose of this study was to improve the yield of the cholesterol oxidase by recombinant DNA techniques. In this study, we examined the overexpression of choB in E. coli and S. lividans host-vector systems, and succeeded in producing the active enzyme in large amounts by S. lividans.…”

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confidence: 99%

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Hyperexpression and Analysis ofchoBEncoding Cholesterol Oxidase ofBrevibacterium sterolicuminEscherichia coliandStreptomyces lividans.

Ohta

Fujishiro

Yamaguchi

et al. 1992

Bioscience, Biotechnology, and Biochemistry

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Section: Expression Of Chob By the Lac Promoter In E Colimentioning

confidence: 76%

Section: Methodsmentioning

confidence: 99%

Section: Construction Of P Lasm Ids For the Expression Of Chob In Smentioning

confidence: 99%

“…Brevibacterium sterolicum A TCC 21387 produces cholesterol oxidase in the culture filtrate. 2) We previously cloned the gene (choB) 8) and analyzed the nucleotide (nt) sequence.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Hyperexpression and Analysis ofchoBEncoding Cholesterol Oxidase ofBrevibacterium sterolicuminEscherichia coliandStreptomyces lividans.

Ohta

Fujishiro

Yamaguchi

et al. 1992

Bioscience, Biotechnology, and Biochemistry

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Cloning, expression, and in silico structural modeling of cholesterol oxidase of Acinetobacter sp. strain RAMD in E. coli

2021

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Rhodococcus

Jones

Goodfellow

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Rho.do.coc'cus. Gr. n. rhodon the rose; N.L. masc. n. coccus (from Gr. masc. n. kokkos grain, seed) coccus; N.L. masc. n. Rhodococcus a red coccus. Actinobacteria / Actinobacteria / Corynebacteriales / Nocardiaceae / Rhodococcus Aerobic, Gram‐stain‐positive to Gram‐stain‐variable, nonmotile actinomycetes that are usually partially acid–alcohol‐fast at some stage of the growth cycle. Rods to extensively branched substrate mycelium may be formed . In all strains the growth cycle begins with the coccus or short rod stage, with different organisms showing a succession of more or less complex morphological stages by which the completion of the cycle is achieved. Cocci may germinate into short rods, form filaments with side projections, show elementary branching, or, in the most differentiated forms, produce extensively branched hyphae. The next generation of cocci and short rods are formed by fragmentation of the rods, filaments, and hyphae. Some strains form sparse microscopically visible aerial hyphae, which may be branched, or aerial synnemata consisting of unbranched filaments that coalesce and project upwards. Colonies may be rough, smooth, or mucoid and pigmented buff, cream, orange, red, or yellow, although colorless variants occur. Chemo‐organotrophic with an oxidative type of metabolism. Catalase‐positive, arylsulfatase‐negative, and sensitive to lysozyme. Most strains grow well on standard media between 15 and 40°C, and use a wide range of organic compounds as sole sources of carbon for energy and growth. DNA G + C content ( mol %): 63–73 (HPLC, T m ). Type species : Rhodococcus rhodochrous (Zopf 1889) Tsukamura 1974, 43 VP .

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Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis

Cited by 28 publications

References 8 publications

Hyperexpression and Analysis ofchoBEncoding Cholesterol Oxidase ofBrevibacterium sterolicuminEscherichia coliandStreptomyces lividans.

Hyperexpression and Analysis ofchoBEncoding Cholesterol Oxidase ofBrevibacterium sterolicuminEscherichia coliandStreptomyces lividans.

Cloning, expression, and in silico structural modeling of cholesterol oxidase of Acinetobacter sp. strain RAMD in E. coli

Rhodococcus

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