Isolation and Identification the Fungus Trichophyton violaceum from Human Skin Specimens in Iraq and Study Efficiency Antibacterial and some Plant Essential Oils

Abstract

This study was aimed to isolate and identify the fungus Trichophyton violaceum from human skin specimens and evaluate the activity of antibacterial and plant essential oils. The results showed of direct microscopy by using KOH examination and morphological identification, nine samples from skin were positive in KOH test. Microscopical examination appeared that colonies of T. violaceum were coarse with abundant aerial mycelium on SDA, growth rates 6-8cm/14 days. The color changes with age from white in the middle to brownish yellow at the edges which was more intense on the reverse side. Susceptibility test to antibacterial showed that 100% of T.Violaceum T2 isolate was resistant to TE (Tetracycline), AK (Amikacin), CTX (Cefotaxime), CFM (Cefixime), CIP (Ciprofloxacin), ERY (Erythromycin) and STR (Streptomycin). Also results showed that all plant essential oils at different concentrations significantly inhibit growth of T. violaceum T2. However, the peppermint oil, Myrrh oil, Cardamom oil, Chamomile oil and Castor oil at highest concentration (25mg / ml) caused highest reduction of mycelia growth (100%) followed by Olive oil (67.3%) and Clove oil (64%) at the same concentration, compared with the control treatment.
 

Investigating <em>Trichophyton violaceum</em> Metabolites and their Effects <em>In Vivo</em>

“…There are no previous detailed studies about examining the composition and toxicity of secondary metabolites isolated from T. violaceum, as well as, three unique pigments distinguish trichophyton species; these pigments were found in just a handful of other fungus species outside the Arthrodermataceae family. In this regard, (Risan, 2016) research revealed that Trychophyton has three primary pigments that have been separated and crystallized; these pigments are derived from the mycelium and referred to as Red Needles, Orange Plates, and Purple Needles. After that, more substantial quantities of these chemical components were separated, and the structures of the compounds were analyzed.…”