Isolation and molecular characterization of Salmonella enterica serovar Enteritidis from poultry house and clinical samples during 2010

Abstract

b s t r a c tA total of 60 Salmonella enterica serovar (ser.) Enteritidis isolates, 28 from poultry houses and 32 from clinical samples, were isolated during 2010. These isolates were subjected to testing and analyzed for antibiotic resistance, virulence genes, plasmids and plasmid replicon types. To assess genetic diversity, pulsed-field gel electrophoresis (PFGE) fingerprinting, using the XbaI restriction enzyme, Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) and plasmid profiles were performed. All isolates from poultry, and 10 out of 32 clinical isolates were sensitive to ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, sulfisoxazole, streptomycin, and tetracycline. Twenty-one of thirty-two clinical isolates were resistant to ampicillin and tetracycline, and one isolate was resistant to nalidixic acid. PFGE typing of sixty ser. Enteritidis isolates by XbaI resulted in 10e12 bands and grouped into six clusters each with similarity from 95% to 81%. The MLVA analysis of sixty isolates gave 18 allele profiles with the majority of isolates displayed in three groups, and two clinical isolates found to be new in the PulseNet national MLVA database. All isolates were positive for 12 or more of the 17 virulence genes mostly found in S. enterica (spvB, spiA, pagC, msgA, invA, sipB, prgH, spaN, orgA, tolC, iroN, sitC, IpfC, sifA, sopB, and pefA) and negative for one gene (cdtB). All isolates carried a typical 58 kb plasmid, type Inc/FIIA. Three poultry isolates and one clinical isolate carried small plasmids with 3.8, 6, 7.6 and 11.5 kb. Ten of the clinical isolates carried plasmids, with sizes 36 and 38 kb, types IncL/M and IncN, and one isolate carried an 81 kb plasmid, type IncI. Southern hybridization of a plasmid with an Inc/FIIA gene probe hybridized one large 58 kb plasmid in all isolates. Several large and small plasmids from poultry isolates were not typed by our PCR-based method. These results confirmed that PFGE fingerprinting has limited discriminatory power for ser. Enteritidis in both poultry and clinical sources. However, the plasmid and MLVA allele profiles were a useful and important epidemiology tool to discriminate outbreak strains of ser. Enteritidis from poultry and clinical samples.Published by Elsevier Ltd.

Molecular Detection, Virulence Genes, Biofilm Formation, and Antibiotic Resistance of Salmonella enterica Serotype enteritidis Isolated from Poultry and Clinical Samples

“…In line with the current study findings, a study conducted by Mezal et al, showed that all isolates were positive for most of the virulence genes found in S. enterica (3).…”

The Simultaneous Administration of a Probiotic or Prebiotic with Live Salmonella Vaccine Improves Growth Performance and Reduces Fecal Shedding of the Bacterium in Salmonella-Challenged Broilers

“…Salmonella infection is one of the most critical bacterial diseases affecting poultry, resulting in a high mortality rate and production losses. Moreover, it is one of the most common foodborne bacterial diseases for humans worldwide, especially Salmonella enteritidis and Salmonella typhimurium [1], including more than 2600 serovars belonging to S. enterica which are Gram-negative and facultative anaerobes belonging to the family Enterobacteriaceae [2]. Cha et al [3] reported that Salmonella infection is widely distributed in poultry in developing countries.…”

Detection of virulence genes and plasmid replicons in Salmonella enterica subsp. enterica, which were allocated during 2014–2017 on the territory of Ukraine

“…Послідовність праймерів Розмір фрагменту Автори invA 5'-TCATCGCACCGTCAAAGGAACC-3' 5'-GTGAAATTATCGCCACGTTCGGGCAA-3' 285 (Borges et al, 2013) agfB 5'-TGATGTTGACAATACTGGGTGCG-3' 5'-CGATATACTGGCATCGTTGGCAT-3' 293 Дане дослідження sefA 5'-GCCGTACACGAGCTTATAGA-3' 5'-ACCTACAGGGGCACAATAAC-3' 310 (Amini et al, 2010) Prt 5'-ATGGGAGCGTTTGGGTTC-3' 5'-CGCCTCTCCACTACCAACTTC-3' 624 (Hong et al, 2008) pFIA 5'-CCATGCTGGTTCTAGAGAAGGTG-3' 5'-GTATATCCTTACTGGCTTCCGCAG-3' 462 (Mezal et al, 2014) pN 5'-GTCTAACGAGCTTACCGAAG-3' 5'-GTTTCAACTCTGCCAAGTTC-3' 559 (Ahmed et al, 2006) pFIIA 5'-CTGTCGTAAGCTGATGGC-3' 5'-CTCTGCCACAAACTTCAGC-3' 270 (Mezal et al, 2014) tetG 5'-CAGCTTTCGGATTCTTACGG-3' 5'-GATTGGTGAGGCTCGTTAGC -3' 844 (Ahmed et al, 2006) Sul1 5'-GTGACGGTGTTCGGCATTCT -3' 5'-TGAGTGCATAACCACCAGCC -3' 378 (Ahmed et al, 2006) 5'-3'CS 5'-GGCATCCAAGCAGCAAGC-3' 5'-AAGCAGACTTGACCTGAT-3' 1009 (Ahmed et al, 2006) Результати та їх обговорення…”