Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh

Hoque, Ashfaqul; Ahmed, Tahmeed; Shahidullah, Mohammad; Hossain, Ahmed; Mannan, Abdul; Noor, Khaled; Nahar, Kamrun; Ilias, Mohammad; Ahmed, Dilruba

doi:10.1016/j.ijfoodmicro.2010.07.019

Cited by 25 publications

(11 citation statements)

References 26 publications

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“…The isolated Cronobacter spp. from commercial PIF samples in Argentina and Bangladesh, respectively, were susceptible to all the tested antibiotics (Terragno et al, 2009;Hoque et al, 2010). In the combined results of molecular typing experiments of the current study, although there was only a minimal difference between the banding patterns of Pattern10 and Pattern09 in the PFGE assay, the Pattern10 strains were resistant to ceftazidime and ampicillin/sulbactam, whereas the Pattern09 strains were susceptible to both antibiotics.…”

Section: Discussionmentioning

confidence: 41%

Isolation and Molecular Typing ofCronobacterspp. in Commercial Powdered Infant Formula and Follow-Up Formula

Pan

Cui²,

Lyu³

et al. 2014

Foodborne Pathogens and Disease

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Cronobacter spp. (Enterobacter sakazakii) are important foodborne pathogens. Infections with this pathogen can lead to neonatal meningitis, necrotizing enterocolitis, and bacteremia. This study examined Cronobacter spp. contamination in commercial powdered infant formulas (PIFs) and follow-up formulas (FUFs) in China. Forty-nine of 399 samples were contaminated with Cronobacter spp. and 10.2% of the isolates were resistant to cefotaxime; in contrast, all of the tested isolates were susceptible to amikacin, amoxicillin/clavulanic acid, cefepime, ciprofloxacin, imipenem, and meropenem. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analyses produced a total of 16 PFGE banding patterns and 11 sequence types (STs), including 7 novel STs. In summary, the rates at which Cronobacter spp. were isolated from commercial PIF and FUF samples in China were relatively high, and the isolated strains exhibited high susceptibility in vitro to most antibiotics. The PFGE method exhibited higher typing capability than the MLST method, and molecular typing results revealed that the contamination of PIF and FUF with Cronobacter spp. in China may be mainly due to the addition of contaminated materials. Thus, the development of more effective control strategies during the manufacturing process is needed.

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Section: Discussionmentioning

confidence: 41%

Isolation and Molecular Typing ofCronobacterspp. in Commercial Powdered Infant Formula and Follow-Up Formula

Pan

Cui²,

Lyu³

et al. 2014

Foodborne Pathogens and Disease

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“…Since Cronobacter spp. give rise to serious health issues and may even cause high mortality in susceptible individuals like newborns and children, they have been extensively investigated in food products appealing to this age group, like milk powder and infant foods (Gökmen et al, 2010;Yao et al, 2016;Hoque et al, 2010). However, no comprehensive studies are available with respect to the occurrence and prevalence of these bacteria in meat and meat products, fruits and vegetables and ready-to-eat foods in Turkey.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Cronobacter spp. in various foodstuffs and identification by multiplex PCR

Aksu

Altunatmaz

Issa³

et al. 2019

Food Sci. Technol

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“…Thus, the International Commis-sion for Microbiological Specifications for Foods (IC-MSF) ranked Cronobacter spp. as a "severe hazard for restricted populations, life threatening or substantial chronic sequelae or long duration" (Hoque et al, 2010). Cronobacter spp., mostly C. sakazakii, can be isolated from a wide range of food sources (Friedemann, 2007;Joseph and Forsythe, 2012); however, many infections caused by this opportunistic pathogen have been epidemiologically linked to contaminated powdered infant formula (PIF; Muytjens et al, 1983;Arseni et al, 1987;Simmons et al, 1989;Ray et al, 2007;Li et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Mining for sensitive and reliable species-specific primers for PCR for detection of Cronobacter sakazakii by a bioinformatics approach

Chen

Tao

Bie

et al. 2015

Journal of Dairy Science

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Although several studies have reported PCR assays for distinguishing Cronobacter sakazakii from other species in the genus, reports regarding assay sensitivity and specificity, as well as applications for food testing, are lacking. Hence, the objective of this study was to develop a sensitive and reliable PCR-based method for detection of C. sakazakii by screening for specific target genes. The genome sequence of C. sakazakii in the GenBank database was compared with that of other organisms using BLAST. Thirty-eight DNA fragments unique to C. sakazakii were identified, and primers targeting these sequences were designed. Finally, 3 primer sets (CS14, CS21, and CS38) were found to be specific for C. sakazakii by PCR verification. The detection limit of PCR assays using the 3 pairs of primers was 1.35 pg/μL, 135 fg/μL, and 135 fg/μL, respectively, for genomic DNA, and 5.5×10(5), 5.5×10(3), 5.5×10(3) cfu/mL, respectively, using pure cultures of the bacteria, compared with 13.5 pg/μLand 5.5×10(5) cfu/mLfor primer set SpeCronsaka, which has been previously described. Cronobacter sakazakii were detected in artificially contaminated powdered infant formula (PIF) by PCR using primer sets CS21 and CS38 after 8h of enrichment. The detection limit was 5.5×10(-1) cfu/10g of PIF. Thus, the PCR assay can be used for rapid and sensitive detection of C. sakazakii in PIF.

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Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh

Cited by 25 publications

References 26 publications

Isolation and Molecular Typing ofCronobacterspp. in Commercial Powdered Infant Formula and Follow-Up Formula

Isolation and Molecular Typing ofCronobacterspp. in Commercial Powdered Infant Formula and Follow-Up Formula

Prevalence of Cronobacter spp. in various foodstuffs and identification by multiplex PCR

Mining for sensitive and reliable species-specific primers for PCR for detection of Cronobacter sakazakii by a bioinformatics approach

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