Isolation and molecular properties of creatine kinase from carp white muscle

Subunit-selective chemical modifications of creatine kinase. Evidence for asymmetrical association of the subunits

“…complex. coupled with quantitative end group analyses (Yue et al, 1967) as well as peptide mapping in a variety of different systems (Dance & Watts, 1962;Thomson et al, 1968;Gosselin-Rey & Gerady, 1970;Kumudavalli et al, 1970; Atherton & Thomson, 1969;Focant, 1970), point heavily to an identity of the two polypeptide chains. In particular, the two reactive thiol groups were shown to be identical, in that they both formed part of a unique 25-residue tryptic peptide sequence (Thomson et al, 1964;Atherton et al, 1970;Mahowald, 1965).…”