Isolation and partial characterization of a novel and uncommon two‐chain 64‐kDa ribosome‐inactivating protein from the bark of elder (<i>Sambucus nigra</i> L.)

Benito, F M de; Citores, Lucı́a; Iglesias, Rosario; Ferreras, José M.; Camafeita, Emilio; Méndez, Enrique; Girbés, Tomás

doi:10.1016/s0014-5793(97)00882-x

Cited by 20 publications

(25 citation statements)

References 28 publications

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“…The activity of nigritins on DNA was measured essentially as described elsewhere [16], but using 1 μg of pBlueScript SK + DNA as substrate. Incubation was for 1 h at 30°C.…”

Section: Methodsmentioning

confidence: 99%

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Constitutive and inducible type 1 ribosome‐inactivating proteins (RIPs) in elderberry (Sambucus nigra L.)

et al. 1998

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Two novel highly basic type 1 (single chain) ribosomeinactivating proteins (RIPs) with N-glycosidase activity have been found in elderberries (the fruits of Sambucus nigra L.). Mass spectrometry of these RIPs, which we named nigritins f1 and f2, gave M r values of 24 095 and 23 565, respectively. Both proteins strongly inhibited protein synthesis in rabbit reticulocyte lysates but were inactive against plant ribosomes. Both nigritins have a similar topological activity on pBlueScript SK + DNA as that displayed by dianthin 30. Nigritin f1 is a constitutive RIP since it is present in both green and mature intact elderberries at nearly the same proportion with respect to total fruit protein. By contrast, nigritin f2 is inducible and only appeared in mature intact elderberries. Elderberries also contain two isoforms of a basic nigrin equivalent to the recently found basic nigrin b in elder bark (De Benito et al., FEBS Letters 413 (1997) 85^91).Our results indicate that probably not all plant RIPs exert the same biological function and that this may be determined by the physiological state of the tissue.z 1998 Federation of European Biochemical Societies.

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“…The activity of nigritins on DNA was measured essentially as described elsewhere [16], but using 1 μg of pBlueScript SK + DNA as substrate. Incubation was for 1 h at 30°C.…”

Section: Methodsmentioning

confidence: 99%

“…With the exception of basic nigrin b [16], all type 2 RIPs isolated from Sambucus species to date bind d ‐galactose and derivatives. We have found that upon removal of d ‐galactose‐binding proteins from a saline extract of elderberries by affinity chromatography the unretained fraction strongly inhibits protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Constitutive and inducible type 1 ribosome‐inactivating proteins (RIPs) in elderberry (Sambucus nigra L.)

et al. 1998

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“…S. ebulus contains heterodimeric type 2 RIPs in the leaves (ebulin l) [25], rhizome [29], and fruits [30]. S. nigra contains heterodimeric type 2 RIPs in all parts of the plant studied: Bark (e.g., nigrin b) [24,31,35,36], leaves [37], fruits [26,28] and seeds [27]. The bark of S. sieboldiana and S. racemosa also contain heterodimeric type 2 RIPs [32,39].…”

Section: Ribosome Inactivating Proteins From Sambucusmentioning

confidence: 99%

“…The B chain allows rapid internalization of the type 2 RIP into the eukaryotic cell, translocation of the A chain into the cytosol, and inactivation of the ribosomes, and for this reason current type 2 RIPs are very toxic proteins. However, a number of nontoxic type 2 RIPs were found in some species from the genus Sambucus [24,25,26,27,28,29,30,31,32]. …”

Section: Introductionmentioning

confidence: 99%

Use of Ribosome-Inactivating Proteins from Sambucus for the Construction of Immunotoxins and Conjugates for Cancer Therapy

Ferreras

Citores

Iglesias

et al. 2011

Toxins

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The type 2 ribosome-inactivating proteins (RIPs) isolated from some species belonging to the Sambucus genus, have the characteristic that although being even more active than ricin inhibiting protein synthesis in cell-free extracts, they lack the high toxicity of ricin and related type 2 RIPs to intact cells and animals. This is due to the fact that after internalization, they follow a different intracellular pathway that does not allow them to reach the cytosolic ribosomes. The lack of toxicity of type 2 RIPs from Sambucus make them good candidates as toxic moieties in the construction of immunotoxins and conjugates directed against specific targets. Up to now they have been conjugated with either transferrin or anti-CD105 to target either transferrin receptor- or endoglin-overexpressing cells, respectively.

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“…Therefore, due to B-chain, type-2 RIPs are among the most potent cytotoxic agents known [3]. In fact, type-2 RIPs (nigrin bb, SNLRP) which lack sugar binding activity are non-toxic [4,5]. Further, type-1 RIPs, lacking a B-chain, enter into cells with difficulty and, consequently, have a relatively low toxicity.…”

Section: Introductionmentioning

confidence: 99%

A cytotoxic type-2 ribosome inactivating protein (from leafless mistletoe) lacking sugar binding activity

Das

Sharma

Mishra

2011

International Journal of Biological Macromolecules

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Isolation and partial characterization of a novel and uncommon two‐chain 64‐kDa ribosome‐inactivating protein from the bark of elder (Sambucus nigra L.)

Cited by 20 publications

References 28 publications

Constitutive and inducible type 1 ribosome‐inactivating proteins (RIPs) in elderberry (Sambucus nigra L.)

Constitutive and inducible type 1 ribosome‐inactivating proteins (RIPs) in elderberry (Sambucus nigra L.)

Use of Ribosome-Inactivating Proteins from Sambucus for the Construction of Immunotoxins and Conjugates for Cancer Therapy

A cytotoxic type-2 ribosome inactivating protein (from leafless mistletoe) lacking sugar binding activity

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