Isolation and Partial Characterization of Bioactive Fucoxanthin fromHimanthalia elongataBrown Seaweed: A TLC-Based Approach

Abstract

Seaweeds are important sources of carotenoids, and numerous studies have shown the beneficial effects of these pigments on human health. In the present study, Himanthalia elongata brown seaweed was extracted with a mixture of low polarity solvents, and the crude extract was separated using analytical thin-layer chromatography (TLC). The separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against Listeria monocytogenes bacteria using TLC bioautography approach. For bio-autography, the coloured band on TLC chromatogram was visualized after spraying with DPPH and triphenyl-tetrazolium chloride reagents which screen antioxidant and antimicrobial compounds, respectively, and only one active compound was screened on the TLC plate. Preliminary identification of this active compound was done by comparing its colour and R f (retention factor) value with the authentic fucoxanthin standard. Further, the active compound was purified using preparative TLC. This purified compound showed a strong antioxidant (EC50: 14.8 ± 1.27 µg/mL) and antimicrobial (inhibition zone: 10.27 mm, 25 µg compound/disc) activities, which were examined by DPPH scavenging and agar disc-diffusion bioassay, respectively. The bioactivity shown by the purified compound was almost similar to the fucoxanthin standard. The characteristic UV-visible and FT-IR spectra of the purified active compound completely matched with the standard. Hence, the main active compound in H. elongata was identified as fucoxanthin.

Seasonal variation of total lipid, fatty acids, fucoxanthin content, and antioxidant properties of two tropical brown algae (Nizamuddinia zanardinii and Cystoseira indica) from Iran

“…Purified fucoxanthin was determined by Fourier transform infrared spectroscopy (FT-IR). The characteristic wave number of specific functional group identified in purified sample was: OH group (3400 cm −1 ), C-H stretch (3005-2850 cm −1 ), allene (1924 cm −1 ), C═O acetate (1732 cm −1 ), conjugated C═O (1645 cm −1 ), CH 2 stretch (1605-1455 cm −1 ), geminal methyl (1380-1360 cm − 1 ), C-O acetate (1338, 1258, and 1249 cm −1 ), and trans-distributed -C═C-(1210-963 cm −1 ) which agreed with the previous reported data (Haugan et al 1992;Rajauria and Abu-Ghannam 2013).…”

Evaluation of antibacterial and antioxidant activity of extracts of endophytic fungi isolated from Indonesian Zingiberaceous plants

“…This method was a quick-approach (Rajauria and Abu-Ghannam 2013), fast, cheap and better bioassay-directed fractionation of bioactive compounds (Hamburger and Cordell 1987) and enabled rapid progress for quick detection of new antimicrobial compounds (Suleimana et al 2010). The growth inhibition of bacteria tested was performed by the presence of clear zone around the extract against the purple background on TLC plate.…”

Antifungal Potential in Crude Extracts of Five Selected Brown Seaweeds Collected from the Western Libya Coast

“…In Brazil, Peres, et al (2013) reported that ten seaweed extracts significantly inhibited the Colletotrichum lagenarium growth, but not inhibited significantly the Aspergillus flavus growth [13]. In Ireland, Rajauria and Abu-Ghannam (2013) screened antimicrobial activity and bioactive compounds of Himanthalia elongate (Brown Seaweed) [14]. In India, Indira, et al (2013) displayed the antifungal property of seaweed Halimeda tuna against nine fungal strains (Aspergillus niger, Aspergillus flavus, Alternaria alternaria, Candida albicans, Epidermophyton floccossum,Trichophyton mentagrophytes, Trichophyton rubrum, Pencillium sp.…”