Isolation and pathogenic variability of Colletotrichum falcatum causing red rot in sugarcane

Patel, Prittesh; Amaresan, N.; Rushabh, S.; Krishnamurthy, R.; Bhasker, V. V.

doi:10.1007/s41348-016-0044-x

Cited by 12 publications

(10 citation statements)

References 7 publications

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“…Further, there would be possibility of a variety of microorganisms associated with roots of different cultivars. In view of this, in the present study different sugarcane varieties were selected in the range of resistance to highly susceptible categories for the isolation of rhizosphere bacteria which have the ability to control highly virulent red rot strains cfCHA, cfNAV, and cf8436 [19].…”

Section: Discussionmentioning

confidence: 99%

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Molecular identification and biocontrol activity of sugarcane rhizosphere bacteria against red rot pathogen Colletotrichum falcatum

Patel

Shah

Joshi

et al. 2019

Biotechnology Reports

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Highlights A total of 226 isolates were screened against three strains (cfNAV, cfCHA and cf8436) of C. falcatum by dual culture technique. Selected Twenty-Six bacteria characterized of morphology, biochemical activity, PGP activity, antifungal potential and 16S rRNA gene sequence. These isolates belonged to proteobacteria (13), firmicutes (10) and bacterioidetes (03) respectively. Ochrobactrum intermedium (TRD14), Acinetobacter sp (PK9), Bacillus sp (RSC29 and KR91) and Escherichia sp (VRE34) selected for green house study. The most promising results in suppression of the disease as well as plant growth were observed in treatment withVRE34. The plant height and stem diameter were increased from 13.27 ± 0.67 inches to 24.03 ± 1.40 inches and from 6.07 ± 0.45 mm to 9.87 ± 0.93 mm. Isolates identified in this study could be used as an alternative to chemical fungicides to control red rot pathogen of sugarcane plants. However, detailed investigations on their inoculations in the field to confirm its growth promotion potency and biocontrol efficacy under natural environmental and soil conditions shall make these strains as important bioinoculants for integrated disease management of red rot disease in sugarcane.

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Section: Discussionmentioning

confidence: 99%

“…C. falcatum strains cfNAV, cfCHA, and cf8436 used in this study were maintained on potato dextrose agar (PDA) slants at 4 °C. Strains cfNAV and cfCHA were chosen according to their higher virulence shown in our previous study [19]. Cf8436 was used as a reference strain obtained from Sugarcane Breeding Institute (Coimbatore, Tamil Nadu, India).…”

Section: Methodsmentioning

confidence: 99%

Molecular identification and biocontrol activity of sugarcane rhizosphere bacteria against red rot pathogen Colletotrichum falcatum

Patel

Shah

Joshi

et al. 2019

Biotechnology Reports

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“…Studies at the molecular level are essential to understand genetic diversity and relationships among accessions. In our previous study we have characterized these South Gujarat C. falcatum accessions on the basis of morphology and virulence pattern [16]. Since, the virulence assays are subject to ecological variations and sometimes leading to disease escape and misinterpretation.…”

Section: Discussionmentioning

confidence: 99%

“…Ten accessions of C. falcatum were isolated from red rot infected stems of nine sugarcane cultivars used in present study [16]. Accessions one to nine were collected different region of South Gujarat and Cf8436 was obtained from Sugarcane Breeding Institute, Coimbatore (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Assessment of genetic diversity in Colletotrichum falcatum Went accessions based on RAPD and ISSR markers

Patel

Rajkumar

Parmar

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Sugarcane is susceptible to red rot disease caused by phytopathogenic fungus Colletotrichum falcatum Went which ultimately affect the economy of farmers as well as sugar based industry. One of the various ways to control this devastating disease is to develop disease resistance sugarcane cultivar and this requires the complete understanding of genetic makeup of pathogen. Although South Gujarat is well known sugarcane cultivating area, less published data can be found about PCR-based genetic diversity in prevalent C. falcatum accessions. So, present investigation aims at finding molecular variation among the ten accessions of C. falcatum using RAPD and ISSR molecular markers. A total of 35 RAPD and 39 ISSR primers were screened across 10 C. falcatum accessions, of which 15 RAPD and 21 ISSR primers have showed consistent amplification. Statistics related to genetic variation were estimated using NTSYS-PC by means of Dice’s coefficient. The results revealed 80.6% and 68.07% polymorphism and similarity coefficient ranged from 0.43 to 0.91 and 0.73 to 0.93 in RPAD and ISSR analysis respectively. The dendrogram generated using RAPD, ISSR and combined RAPD-ISSR grouped accessions into different clusters which reveal considerable level molecular variation among the C. falcatum accessions. It is also evident from PCA plots that accessions are rather dispersed with tested marker systems indicating good genetic base. So, in nut shell, we found considerable genetic variation and relatedness within C. falcatum accessions collected from different areas of south Gujarat, India using RAPD and ISSR markers.

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“…Moreover, for isolate UEL27, relevant cultural differences were observed and it also proved to be genetically more distant from the others. Despite the low variation observed among the partial sequences of the genes studied in the 11 isolates, the observation of polymorphism through molecular markers evidence stout this studies are essential to understand the genetic relationships among isolates, as mentioned in the studies of Wijesekara, Aggarwal & Agarwal (2005) and Prittesh et al (2016), who reported high genetic diversity within the Colletotrichum species. A correct identification of the species, knowledge of pathogen variability and infection strategies are important steps to plan an efficient anthracnose control and management strategies for Capsicum spp.…”

Section: Discussionmentioning

confidence: 92%

Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil

Giacomin

Ruas

Baba

et al. 2021

PeerJ

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Anthracnose is a disease caused by Colletotrichum spp., one of the world’s most damaging sweet and chili pepper pathogens, especially in tropical and subtropical regions. In the state of Rio de Janeiro, anthracnose is one of the main obstacles for pepper crops. However, to date no research has focused on the identification and characterization of the pathogen, which is fundamental to understand the scope of the disease in the state. Thus, the correct identification of the fungal species and pathogenicity studies can provide important support for disease management and control, apart from identifying possible resistance sources for exploitation in peppers breeding programs. In this study, 11 Colletotrichum isolates were collected from peppers with typical symptoms in the Rio de Janeiro state. These isolates were characterized based on morpho-cultural characteristics and sequencing data from five regions (ITS, ACT, CAL, β-TUB and GAPDH), and the genetic variability was estimated by AFLP markers. Simultaneously, microscopy images of the colonization by the fungal species on unripe Capsicum annuum fruits were taken. Pathogenicity was tested and resistance sources were sought by means of infection of ripe and unripe fruits of 50 Capsicum baccatum accessions. The resulting data showed that all isolates belong to Colletotrichum scovillei specie. About the pathogenicity of Capsicum baccatum, differentiated, stage-specific responses, with higher resistance of ripe fruits were recorded. In addition, four possible sources of Colletotrichum scovillei resistance were detected among the tested accessions. The combination of these data can contribute to future studies on the interaction of Colletotrichum scovillei-Capsicum spp., a research line that is still unexploited in the main areas of this anthracnose fungus.

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Isolation and pathogenic variability of Colletotrichum falcatum causing red rot in sugarcane

Cited by 12 publications

References 7 publications

Molecular identification and biocontrol activity of sugarcane rhizosphere bacteria against red rot pathogen Colletotrichum falcatum

Molecular identification and biocontrol activity of sugarcane rhizosphere bacteria against red rot pathogen Colletotrichum falcatum

Assessment of genetic diversity in Colletotrichum falcatum Went accessions based on RAPD and ISSR markers

Phenotypic, molecular and pathogenic characterization of Colletotrichum scovillei infecting Capsicum species in Rio de Janeiro, Brazil

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