ISOLATION AND PRELIMINARY CHARACTERIZATION OF BACTERIOPHAGES FOR
            <i>BACILLUS SUBTILIS</i>

Romig, W. R.; Brodetsky, Anna M.

doi:10.1128/jb.82.1.135-141.1961

Cited by 84 publications

(34 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Southern analysis of the genome of this phage revealed that it shares significant homology with the virulent bacteriophage E79 . These data suggest that although UTl is apparently virulent on well-fed hosts, starvation conditions found in the aquatic habitat favour the establishment of a pseudolysogenic relationship between the bacteriophage and its host bacterium, much like the one described by Romig & Brodetsky (1961) in soil bacilli. In some aquatic environments as many as 45% of the presumptive P.…”

Section: Introductionmentioning

confidence: 65%

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

1994

View full text Add to dashboard Cite

A pseudolysogenic, generalized transducing bacteriophage, UT1, isolated from a natural freshwater habitat, is capable of mediating the transfer of both chromosomal and plasmid DNA between strains of Pseudomonas aeruginosa. Several chromosomal alleles from three different P. aeruginosa strains were found to transduce at frequencies from 10(-8) to 10(-10) transductants per PFU at multiplicities of infection (MOI) between 0.1 and 1. Transduction frequencies of certain alleles increased up to 1000-fold as MOIs were decreased to 0.01. UT1 is also capable of transducing plasmid DNA to indigenous populations of microorganisms in natural lake-water environments. Data obtained in this study suggest that environmentally endemic bacteriophages such as UT1 are formidable transducers of naturally occurring microbial communities. It should be possible to develop model systems to test transduction in freshwater environments using components derived exclusively from these environments.

show abstract

Section: Introductionmentioning

confidence: 65%

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

1994

View full text Add to dashboard Cite

show abstract

“…Although many phages infecting Bacillus spp. have been isolated (Romig and Brodetsky 1961;Ackermann et al 1978;Strauch et al 1989), B. cereus phages have not received much attention. This may be because disease caused by B. cereus is considered not as serious as that caused by other foodborne pathogens and other Bacillus spp., such as B. anthracis.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of phages infecting Bacillus cereus

Lee

Billington

Hudson

et al. 2011

Letters in Applied Microbiology

View full text Add to dashboard Cite

Aim: To isolate and characterize bacteriophages (phages) that infect the foodborne pathogen Bacillus cereus. Methods and Results: Two phages were isolated from soil based on their ability to form plaques on four indicator hosts including Bacillus thuringiensis subsp. israelensis, and three isolates of B. cereus. The purified phages were characterized by morphology, host range, single‐step growth curves and restriction enzyme digestion profiles. The phages appeared to be of the Myoviridae family based on their structure in electron micrographs. The phages lysed bacteria of several species, produced average burst sizes of 322 and 300 phages per infected cell, and both had genomes over 90 kb. The phages were chloroform‐resistant and stable at 4°C. They reduced the concentration of B. cereus in mashed potatoes by >6 log10 CFU ml−1 within 24 h at room temperature, when applied at a high concentration. Conclusions: The relatively narrow host range within B. cereus might mean that these phages need to be used as part of a ‘cocktail’ of phages for biocontrol, but their efficacy for the control of their host in food was demonstrated. Significance and Impact of the Study: This is the first report of biocontrol by phages of B. cereus in food.

show abstract

“…Certain virulent phages can exist inside dormant hosts in an arrested stage of the lytic cycle, which can resume when the host recommences vegetative growth. This state is known as pseudolysogeny [13].…”

Section: Introductionmentioning

confidence: 99%

Phage-host interactions in soil

Marsh

Wellington

1994

FEMS Microbiology Ecology

101

View full text Add to dashboard Cite

Phages are abundant and ubiquitous in nature, and are therefore important components of microbial communities. They can impact on host populations in several ways, including predation and alteration of host phenotype by genetic interactions. The dynamic survival of phage populations in soil requires infective interactions with host populations which must be undergoing growth. Hence survival is limited by the activity of soil bacteria, and phage populations must adopt strategies to overcome periods of inactivity. One of the most effective strategies is the lysogenic cycle of temperate phages. It is argued here that lysogeny in soil has a distinct advantage over virulence for phage and host survival, as opposed to aquatic ecosystems where virulence seems a more successful strategy for phage populations.

show abstract

ISOLATION AND PRELIMINARY CHARACTERIZATION OF BACTERIOPHAGES FOR BACILLUS SUBTILIS

Cited by 84 publications

References 9 publications

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

Transduction of a freshwater microbial community by a new Pseudomonas aeruginosa generalized transducing phage, UT1

Isolation and characterization of phages infecting Bacillus cereus

Phage-host interactions in soil

Contact Info

Product

Resources

About