Isolation and preliminary characterization of a novel Helicobacter species from swine

Krakowka, Steven; Ringler, Susan S.; Flores, Joel; Kearns, Robert J.; Eaton, K. A.; Ellis, John

doi:10.2460/ajvr.2005.66.938

Cited by 21 publications

(41 citation statements)

References 56 publications

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“…and once or twice curved Helicobacter pylori-like bacteria) can colonize the stomach of pigs [5,7,13]. Findings from this study of the prevalence of Helicobacter spp.…”

Section: Discussionmentioning

confidence: 95%

“…Gastritis is the result of a natural infection with H. pylori in humans [26] and conventional pigs [13] or an experimental induced infection in gnotobiotic piglets [3,14,15], miniature pigs [12] and conventional piglets [22]. In both, humans and pigs, the presence of H. pylori correlates with an inflammatory response, but there are differences in inflammatory cell population.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteria that colonize the stomach, including curved or S-shaped Helicobacter-like organisms (HLO) and spiral-shaped Gastrospirillum-like organisms (GLO) are isolated from humans [11,26] and several non-human species, including pigs [1,13,23]. Infection with H. pylori is correlated with histological characteristics of active and chronic gastritis.…”

Section: Introductionmentioning

confidence: 99%

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Correlation between Different Helicobacter Morphotypes and Histological Changes in Pig Gastric Mucosa

Pejčinovska

Lalošević

et al. 2018

Acta Scientiae. Vet.

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Background: Two distinct morphologic types of bacteria which belong to the Helicobacter species, have been described in pigs: once or twice curved Helicobacter pylori-like bacteria (HLO) and the multicoiled, Gastrospirillum-like bacteria (GLO). The objective of this study was to determine the incidence of Helicobacter spp. using modified Giemsa stain and to define the relationship between presence of Helicobacter spp. and histopathological changes of gastric mucosa in pigs. Materials, Methods & Results: A total of 120 pig stomachs (60 from intensive and 60 from extensive breeding) were enrolled in this study and 240 fragments of fundic and pyloric mucosa were taken for histopathological examination. By modified Giemsa staining of gastric mucosa, Helicobacter-like organisms were confirmed in 4/60 (6.67%) of pigs in intensive and 5/60 (8.33%) of pigs in extensive breeding. The incidence of tightly spiral shaped Gastrospirillum-like organisms in pigs of intensive and extensive breeding were 5/60 (8.33%) and 9/60 (15%), respectively. The severity of gastritis was scored to the Sydney System with some modifications. There was no significant difference between HLO-positive and HLO-negative fundic mucosa in pigs of both breeding systems. In contrary, there was significant difference between HLO-positive and HLO-negative pyloric mucosa of pigs in intensive (P < 0.001) and in extensive breeding (P < 0.05). In intensive breeding, there was significant difference between GLO-positive and GLO-negative fundic mucosa (P < 0.05), while there was no significant difference between GLO-positive and GLO-negative pyloric mucosa. Discussion: The prevalence of Helicobacter spp. bacteria were in coherence with literature data. The higher prevalence of both morfological type of bacteria, were considered in pigs in extensive breeding. the hygienic conditions and managment factors in pigs farm are the possible impact for higher bacterial transmission. The association of high prevalence of H. pylori and poor hygienic condition was shown by epidemiological studies conducted on humans. In both, humans and pigs, the presence of H. pylori correlates with an inflammatory response, but there are differences in inflammatory cell population. In H. pylori infected humans, neutrophils composed the bulk of cellular infiltrate, while in pigs, the primary inflammatory cell was the lymphocyte, which is in accordance to results published by others autors. Thus it indicates that different hosts exhibit a different pathohistological response to the Helicobacter spp. infection. In human as well as in veterinary pathology, the fact of the different pathogenicity of various Helicobacter species is well known. In all HLO-positive pyloric mucosa, moderate to severe focal or diffuse infiltration of mononuclear cells and lymphoid follicles with germinal centers, were observed. A similar conclusion was drawn from results of an experimental infection study in pigs. There was significance between HLO-positive and HLO-negative pyloric mucosa in both, intensi...

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“…and once or twice curved Helicobacter pylori-like bacteria) can colonize the stomach of pigs [5,7,13]. Findings from this study of the prevalence of Helicobacter spp.…”

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Correlation between Different Helicobacter Morphotypes and Histological Changes in Pig Gastric Mucosa

Pejčinovska

Lalošević

et al. 2018

Acta Scientiae. Vet.

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“…Others have described this pathogenesis in gastric ulcers lesions (Embaye et al 1990, Krakowka et al 1995, Argenzio & Eisemann 1996, Friendship 2006). In addition, Krakowka et al (2005) suggested that the porcine gastric ulcer disease complex may involve gastric colonization with Helicobacter pylori-like bacteria, but additional studies would help to clarify the association of the bacteria with the gastric ulcers development.…”

Section: Introductionmentioning

confidence: 99%

“…PCV2 is the essential infectious component of postweaning multisystemic wasting syndrome (PMWS) , Meehan et al 2001, Krakowka et al 2005, which is an economically important disease of piglets characterized by wasting and multisystemic lesions, including granulomatous lymphadenitis with lymphoid depletion, hepatitis, pneumonia, and myocarditis , Chae 2004). The demonstration of high or moderate amounts of PCV2 antigen or nucleic acids in close association with characteristic microscopic lesions confirms the diagnosis of PMWS (Sorden 2000).…”

Section: Introductionmentioning

confidence: 99%

Gastric ulcers in pigs affected with postweaning multisystemic wasting syndrome

et al. 2008

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601Pesq. Vet. Bras. 28(12):601-605, dezembro 2008 RESUMO.-[Úlceras gástricas em suínos afetados pela síndrome multissistêmica do definhamento.] Amostras de linfonodos gástricos e os estômagos de 24 leitões selecionados de rebanhos afetados pela síndrome multissistê-mica do definhamento suíno e mortes súbitas por úlceras gástricas foram estudados. Os animais foram selecionados por baixa performance, baixo consumo de alimento e desnutrição. Os estômagos foram abertos, invertidos e classificados, conforme a severidade das lesões presentes na pars oesophagica (porção não-glandular da mucosa gás-trica). Amostras selecionadas foram processadas por mé-todo histológico convencional para coloração de hematoxilina-eosina. Testes imuno-histoquímicos utilizando anticorpos anti-PCV2 (circovírus suíno tipo 2), anti-Helicobacter pylori e anticitoqueratina de largo espectro foram feitos. As alterações macroscópicas na pars oesophagica foram classificadas como de escore 3, 2 e 1 respectivamente em 8, 6 e 5 estômagos. Microscopicamente, foram notados folículos linfóides hiperplásicos, infiltrados linfohistiocitários e focos de necrose na mucosa gástrica. Grandes quanti- Samples of gastric lymph nodes and the stomachs from 24 pigs selected from herds affected by postweaning multisystemic wasting syndrome (PMWS) and sudden death associated with gastric ulcers were studied. Pigs were selected on the basis of unthriftiness, decreased feed intake, and wasting. The stomachs were opened, inverted, and classified into 0-3 score according the severity of the gross lesions present in pars oesophagica (non-glandulargastric mucosa). Selected samples were processed for paraffin embedding and stained with hematoxylin and eosin. Immunohistochemistry using anti-PCV2 (porcine circovírus type 2) antibody, anti-Helicobacter pylori antibody and a wide-spectrum anti-cytokeratin antibody was performed. Gross changes in pars oesophagea were classified according to the severity of lesions as score 3, 2, and 1 in 8, 6, 5 stomachs respectivelly. Microscopically, hyperplastic lymphoid follicles, lymphohistiocytic inflammatory infiltrates and focci of necrosis in the gastric mucosa were common findings. Large amounts of PCV2 antigen were observed in the cytoplasm and nuclei from intralesional cells and debris from the gastric glandular mucosal zone; however, in the fundus, anti-PCV2 immunostaining was restricted to the surface mucosal cells and foveolar compartment. All gastric lymph nodes were positive for PCV2 antigen. Anti-H. pylori immunostaining was seen in eleven cases, mainly in the antrum, on the mucosal surface and foveolar compartment. The association of the anti-PCV2 immunostaining with the glandular mucus-producing cells suggests a role for PCV2 as an additional factor for the swine ulcer development. dades de antígeno do PCV2 foram observadas no citoplasma, núcleo e restos necróticos de células intralesionais das glândulas gástricas nas regiões do antro e cárdia; entretanto, na região do fundo, a marcação anti-PCV2 foi restrita às células da...

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The role of infectious agents in the development of porcine gastric ulceration

Witte

Ducatelle

Haesebrouck

2018

The Veterinary Journal

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Isolation and preliminary characterization of a novel Helicobacter species from swine

Abstract: Findings of this study indicate that development of gastric mucosal ulcers and ulceration of the nonglandular pars esophagea in pigs may be associated with gastric colonization by swine-origin Helicobacter spp, which are similar to H pylori isolated from humans.

Cited by 21 publications

References 56 publications

Correlation between Different Helicobacter Morphotypes and Histological Changes in Pig Gastric Mucosa

Correlation between Different Helicobacter Morphotypes and Histological Changes in Pig Gastric Mucosa

Gastric ulcers in pigs affected with postweaning multisystemic wasting syndrome

The role of infectious agents in the development of porcine gastric ulceration

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