Isolation and purification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C) in Brazilian propolis by supercritical fluid extractions

Lee, Ying-Nong; Chen, Chao-Rui; Yang, Hsin‐Ling; Lin, Chu‐Chieh; Chang, Chieh-Ming J.

doi:10.1016/j.seppur.2006.08.028

Cited by 33 publications

(24 citation statements)

References 20 publications

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“…12 (Brasil, 2001) approves the Technical Regulation on microbiological standards for food and states that the tolerance in fresh pork sausage for coagulase-positive Staphylococcus is 3×10 3 CFU/g; consequently, all the treatments in the present study remained within the legally allowed limit during storage. According to Lee et al (2007) the ethanol extract of propolis has antimicrobial activity in relation to Staphylococcus aureus. …”

Section: Resultsmentioning

confidence: 99%

Preparation and microbiological analysis of Tuscan sausage with added propolis extract

et al. 2016

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The aim of this study was to obtain hydroethanolic extract of propolis by extraction, assisted by focused microwave, and to apply it in Tuscan-style sausage. The extract was used at concentrations of 0.5%, 1.0% and 2.0% (w/v) in the manufacture of the sausage, which was then analyzed in cold storage at 4 °C for 56 days. The following analyses were performed: mesophilic and psychotrophic organisms; coliforms at 35 and 45 °C; positive and negative-coagulase Staphylococcus, sulfite-reducing Clostridium, and Salmonella spp. The results were below the limits established by the Brazilian legislation, with some changes at the end of the study. Consequently, propolis extract prolonged the shelf life of the Tuscan-style sausage for 56 days and it is therefore an ingredient that can be potentially used in the preparation of this product.Keywords: sausage; propolis; extraction.Practical Application: The propolis extract provided microbiological stability to the sausage.

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Section: Resultsmentioning

confidence: 99%

Preparation and microbiological analysis of Tuscan sausage with added propolis extract

et al. 2016

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“…Solid-liquid column purification, a form of partition extraction, has been used in the isolation of bioactive compounds from extracted oil [25][26][27]. In this study, a total 15 g of SC-CO 2 -extracted oil was obtained and diluted with N-hexane in a batch.…”

Section: Column Partition Fractionation Of γ-Oryzanols Oilmentioning

confidence: 99%

Supercritical carbon dioxide extraction of rice bran oil and column partition fractionation of γ-oryzanols

Chen

Wang

et al. 2008

Separation and Purification Technology

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“…Catchpole et al (2004) used sc-CO 2 both as an antisolvent to precipitate high molecular mass components, and also as a solvent to extract the ethanol and the soluble components of the EEP (nondried). Lee et al (2007) extracted DHCA from Brazilian propolis using sc-CO 2 modified with cosolvent, followed by column chromatography, to obtain very pure DHCA. Chen et al (2009), using a sc-CO 2 extract containing 41.2% (wt) DHCA, successfully suppressed growths of human colo-205 cancer cells, although the total yield of the sc-CO 2 extract was relatively low when compared with the extract obtained with ethyl acetate in a Soxhlet apparatus.…”

Section: Introductionmentioning

confidence: 99%

Supercritical CO2 extraction of raw propolis and its dry ethanolic extract

Paviani

Saito

Dariva

et al. 2012

Braz. J. Chem. Eng.

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-Three types of propolis extract were prepared and analyzed with respect to their global extraction yields and with respect to the concentration of the following markers: 3,5-diprenyl-4-hydroxycinnamic acid; 3-prenyl-4-hydroxycinnamic acid; 4-hydroxycinnamic acid and 4-methoxy-3,5,7-trihydroxyflavone. The extract EEP (ethanolic extract of propolis) was obtained by the conventional method from raw propolis using ethanol as solvent. The extracts (SFE) were obtained by supercritical solvent extraction from the raw propolis using supercritical carbon dioxide (sc-CO 2 ), with and without the addition of ethanol as a co-solvent. The fractionated supercritical extracts (FSCE) were obtained by fractionation (extract and raffinate) of the dry EEP with sc-CO 2 . EEP yields of 39.5% were obtained and maximum global extraction yields were 7.3% for SFE with no co-solvent, 51% for SFE with 15% ethanol and 18% for the FSCE extract fraction. The concentrations of the markers in the different extracts differed as a function of the operational parameters, indicating that the addition of co-solvent and the selectivity of sc-CO 2 could be manipulated so as to obtain extracts with the yields and concentrations of interest.

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Isolation and purification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C) in Brazilian propolis by supercritical fluid extractions

Cited by 33 publications

References 20 publications

Preparation and microbiological analysis of Tuscan sausage with added propolis extract

Preparation and microbiological analysis of Tuscan sausage with added propolis extract

Supercritical carbon dioxide extraction of rice bran oil and column partition fractionation of γ-oryzanols

Supercritical CO2 extraction of raw propolis and its dry ethanolic extract

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