ISOLATION AND PURIFICATION OF ANTICANCER PROTEIN EXOTXIN A FROM Pseudomonas aeruginosa

Abstract

Toxins produced from bacteria constitute promising antitumor agents in treating different cancer types. Exotoxin A from Pseudomonas aeruginosa is the highly toxic virulence component that bind to specific cell receptors. This study aimed to purify Exotoxin A from clinically isolated Pseudomonas aeruginosa. A total of 150 bacterial isolates were taken from clinical samples (burn and wounds) and were screened on selective media and identified as P. aeruginosa using biochemical analysis and molecular test by PCR amplification technique utilizing specific primer 16srRNA. Exotoxin A produced from P. aeruginosa were screened and purified by two steps that include gel filtration and ion-exchange chromatography. In this study, 68 isolates were characterized as P. aeruginosa, furthermore, real-time PCR proved that these isolates revealed 100% specificity, sensitivity and had positive amplified bands with a size of 249bp. The concentration of Exotoxin A extracted from P. aeruginosa using Trypticase soy broth was 18 Mg/ml. The percent of Purification and recovery for Exotoxin A was 21.4 %, 40% respectively. Clinical Isolates of Pseudomonas aeruginosa have the potential to produce Exotoxin A that is responsible for pathogenicity.

Cytotoxic Effect of Asparaginase Produce From E.COLI and Conjugated With Zno Nanoparticles on Cancer Cell Lines

“…All samples were identified by morphology, biochemical analysis, and the VITEK-2 System (15). Qualitative screening for asparaginase production: Escherichia coli were examined for L-asparaginase production.…”

Effect of pigments of Pseudomonas aeruginosa on adhering and cytotoxicity of A549 cell line

“…P. aeruginosa bacteria cause cystic fibrosis patients, wounds, burns, immunodeficiency patients, chronic obstructive pulmonary disorder and cancer (Qin et al, 2022) and cause serious infections involving mainly all human organs, including lung infection, pneumonia and urinary tract infection, soft tissue infections in burn and open wound, diabetic foot ulcer and keratitis (Morin et al, 2021), they are associated with serious diseases and infections such as pneumonia (Mohammed and Zgair, 2022). P. aeruginosa bacteria have many virulence factors such as protease, elastase and pyocyanin that cause tissue damage in the bloodstream (Jawad and Rasheed, 2022). as well as having pilli, which has a role in adhering to epithelium and the exoenzyme (S) that promotes adhesion to epithelium cell (Moissenet and Khedher, 2011) and exotoxin A, which is responsible for tissue necrosis (Al-Shwaikh and Alornaaouti, 2018), and has the ability to form a biofilm that has a role in the adhesion of P. aeruginosa bacteria to the living and nonliving surface (Vetrivel et al, 2021).…”