Isolation and Purification of Kudinosides from Kuding Tea by Semi-Preparative HPLC Combined with MCI-GEL Resin

Abstract

Background: Kuding tea, a Traditional Chinese drink, has a history of thousands of years in China. Triterpenoid saponins in Kuding tea are regarded as one of the major functional ingredients. Objective: The aim of this paper is to establish a separation progress for isolation and purification of five triterpenoid saponins (kudinoside A, C, D, F, G) from Kuding tea. Method: Nine type of resins including seven macroporous resins and two MCI-GEL resins were firstly used for purifying triterpenoid saponins by the adsorption and desorption tests. Further dynamic adsorption/desorption experiments were carried out to obtain the optimal parameters for the five targeted saponins. Then the purification of five triterpenoid saponins (kudinoside A, C, D, F, G) was completed by semi-preparative high performance liquid chromatograph (semi-pHPLC). Results: As of optimized results, the HP20SS MCI-GEL was selected as the optimal one. The data also showed that 65.24 mg of refined extract including 7.04 mg kudinoside A, 3.52 mg kudinoside C, 4.04 mg kudinoside D, 4.13 mg kudinoside F, 34.45 mg kudinoside G, could be isolated and purified from 645.90 mg of crude extract in which the content of five saponins was 81.51% and the average recovery reached 69.76%. The final contents of five saponins increased 6.91-fold compared to the crude extract. Conclusion: The established separation progress was highly efficient, making it a potential approach for the large-scale production in the laboratory and providing several markers of triterpenoid saponins for quality control of Kuding tea or its processing products.

In‐depth analysis of the xanthine oxidase inhibitors of Cicer arietinum L.‐based receptor–ligand affinity coupled with complex chromatography

“…Compared with traditional separation methods (macroporous resin adsorption and precipitation methods), 23 CCC is a stable, repeatable, solid‐free, liquid–liquid partition chromatography technique and is extensively used in the separation and purification of complex compounds 24–26 . Semi‐preparative high‐performance liquid chromatography (semi‐preparative HPLC), as a classical chromatography method is also widely applied in the separation and purification of natural products 27 . Moreover, the combination of CCC of liquid–liquid system and semi‐preparative HPLC of liquid–solid system has complementary advantages for separating bioactive compounds from crude extracts of C. arietinum L., 28–30 demonstrating its promising potential for industrial applications 31 …”

“…[24][25][26] Semi-preparative highperformance liquid chromatography (semi-preparative HPLC), as a classical chromatography method is also widely applied in the separation and purification of natural products. 27 Moreover, the combination of CCC of liquid-liquid system and semi-preparative HPLC of liquidsolid system has complementary advantages for separating bioactive compounds from crude extracts of C. arietinum L., [28][29][30] demonstrating its promising potential for industrial applications. 31 Herein, we screened target compounds by employing the UF assay based on receptor-ligand affinity, which was expected to screen potential XOD inhibitors out.…”