Isolation and purification of lactoferrin from colostrum of Malabari goats

Abstract

Lactoferrin, an iron-binding glycoprotein, primarily present in milk, has been shown to be involved in various physiological and protective function including roles in iron homeostasis, cell proliferation, antibacterial, antifungal, antiviral, antioxidant, immunomodulatory and anticancer activities. The present study focused on the isolation and purification of lactoferrin of Malabari goats, an indigenous goat b reed of Kerala. Lactoferrin is isolated from colostrum samples by cation exchange chromatography using CM Saphadex C-50 column. The isolated protein was identified and confirmed by SDS-PAGE in terms of its molecular weight. A single 80 kDa Coomassie Brilliant Blue-stained band observed in the electrophoretic profile ascertained the purity of the protein isolated. The results of the study point to a simple one step method to obtain pure lactoferrin from goats.

Polyfunctional properties of goat colostrum proteins and their use

“…The main antimicrobial protein in goat colostrum is lactoferrin. The molecular weight of lactoferrin is approximately 80 kDa [15].…”

Effect of Malabari goat lactoferrin on tumour growth kinetics in experimentally induced tumours in mice

“…The antimicrobial potential of Lf of native goat breeds of Kerala viz. Attappady Black and Malabari was found to be significantly superior to that of crossbred goats (Vijayan et al, 2017). This study aims to furnish insights to the anticancer activity of Malabari goat Lf (MgLf) with respect to tumour growth kinetics.…”