Lactoferrin, an iron-binding glycoprotein, primarily present in milk, has been shown to be involved in various physiological and protective function including roles in iron homeostasis, cell proliferation, antibacterial, antifungal, antiviral, antioxidant, immunomodulatory and anticancer activities. The present study focused on the isolation and purification of lactoferrin of Malabari goats, an indigenous goat b reed of Kerala. Lactoferrin is isolated from colostrum samples by cation exchange chromatography using CM Saphadex C-50 column. The isolated protein was identified and confirmed by SDS-PAGE in terms of its molecular weight. A single 80 kDa Coomassie Brilliant Blue-stained band observed in the electrophoretic profile ascertained the purity of the protein isolated. The results of the study point to a simple one step method to obtain pure lactoferrin from goats.

Isolation and in vitro propagation of Infectious Hypodermal Hematopoietic Necrosis Virus (IHHNV/ PstDVI) in PmLyO-Sf9 could be successfully performed. After few hours of post inoculation with the virus, cytopathic changes such as (a) clustering (b) enlargement (c) syncytium formation (d) granulation (e) vacuole formation (f) tapering (g) irregular plasma membrane with extended tails (h) detachment (i) cell death and cellular debris formation were observed. Expression of viral genes, presence of virions and cytological changes demonstrated through TEM suggested replication of the virus in the shrimp -insect hybrid cell line. The virus could be puri ed by ultracentrifugation, negatively stained, and demonstrated under electron microscope. The same was found to be infective both in vitro and in vivo. This development opens avenues for the study of basic molecular mechanism of IHHNV infection, pathogenesis and replication kinetics much required for developing antiviral strategy in aquaculture.

Isolation and in vitro propagation of Infectious Hypodermal Hematopoietic Necrosis Virus (IHHNV/ PstDVI) in PmLyO-Sf9 could be successfully performed. After few hours of post inoculation with the virus, cytopathic changes such as (a) clustering (b) enlargement (c) syncytium formation (d) granulation (e) vacuole formation (f) tapering (g) irregular plasma membrane with extended tails (h) detachment (i) cell death and cellular debris formation were observed. Expression of viral genes, presence of virions and cytological changes demonstrated through TEM suggested replication of the virus in the shrimp - insect hybrid cell line. The virus could be purified by ultracentrifugation, negatively stained, and demonstrated under electron microscope. The same was found to be infective both in vitro and in vivo. This development opens avenues for the study of basic molecular mechanism of IHHNV infection, pathogenesis and replication kinetics much required for developing antiviral strategy in aquaculture.